MPG-based nanoparticle: An efficient delivery system for enhancing the potency of DNA vaccine expressing HPV16E7

Saleh, Tayebeh; Bolhassani, Azam; Shojaosadati, Seyed Abbas; Aghasadeghi, Mohammad Reza

doi:10.1016/j.vaccine.2015.05.015

Cited by 51 publications

(41 citation statements)

References 27 publications

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“…The formation of complexes between peptide and DNA (pcDNA‐Nef, pcDNA‐Hsp20‐Nef, pEGFP‐Nef, and pEGFP‐Hsp20‐Nef) was assessed by gel retardation assay. To evaluate the stability of four complexes against DNA nucleases, DNase I was added to the complexes (at certain ratios obtained from gel retardation assay: 0, 2, 5, and 10) with a final concentration of 1.37 U/ μg DNA and the mixtures were incubated at 37°C for 1 hr followed by the addition of stop solution (200 mM sodium chloride, 20 mM EDTA and 1% SDS; Sigma, Germany) . For assessment of the serum stability, the nanoparticles at the N/P ratios obtained from gel retardation assay (0, 2, 5, and 10) were exposed to 10% serum and incubated for 5 hr at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

et al. 2019

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Cell penetrating peptides (CPPs) can potently transport therapeutic molecules to target cells for treatment of a variety of diseases. Thus, their use is critical to improve therapeutic vaccines. Histidine‐rich nona‐arginine (HR9) and primary amphipathic peptide (MPG) showed the ability to transfer DNA into the cells. Moreover, the peptide derived from the C‐terminal of the tumor suppressor protein p14ARF (M918) and arginine‐rich peptide (penetratin) were utilized to deliver polypeptides and proteins into the living cells. In this study, the immunostimulatory properties of HIV‐1 Nef DNA and protein constructs were evaluated using small heat shock protein 20 (sHsp20) and Freund's emulsion as an adjuvant, and four CPPs (HR9, MPG, M918, and penetratin) as a gene or protein carrier in BALB/c mice. Our data indicated that the HR9/DNA, MPG/DNA, M918/protein, and penetratin/protein complexes formed the stable nanoparticles that were effectively delivered in HEK‐293T cell line at certain ratios. Moreover, a heterologous Hsp20‐Nef DNA + MPG prime/rHsp20‐Nef protein+M918 boost regimen significantly elicited higher levels of IgG2a, IgG2b, IFN‐gamma, and Granzyme B directed toward Th1 responses in a long period (3 months) after the last immunization compared to other groups. Furthermore, the effective role of Hsp20 was detected as a natural adjuvant in enhancing immune responses against HIV‐1 Nef antigen. These findings demonstrated that the simultaneous use of M918 and MPG CPPs as protein and gene carriers improves HIV‐1 Nef‐specific B‐ and T‐cell immune responses as a promising approach for development of HIV‐1 monovalent vaccine.

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Section: Methodsmentioning

confidence: 99%

Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

et al. 2019

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“…65 In our recent study, MPG peptide which is a short amphipathic peptide carrier was used for in vitro and in vivo delivery of HPV16 E7 DNA as a model antigen. 36 The results of this study demonstrated several properties of MPG that propose it as an ideal vector for use in DNA vaccine delivery. As it is shown in Figure 2, MPG was able to interact and form stable non-covalent NPs with DNA through electrostatic interactions, which take place between the negative charges of the nucleic acids (phosphate groups) and the positively charged moiety of MPG.…”

Section: Cellular and Nuclear Localization Of Dna Vaccinesmentioning

confidence: 58%

“…In fact, immunization with the 200 nm NPs favored Th1 type immune responses denoted by production of IFN-g, whereas immunization with the 700 nm particles induced a higher antibody titer. 36 Along with size, charge and the nanoparticle's surface properties are also important. In general, cationic particles are taken up into DCs and macrophages much more readily than those with an overall negative surface charge due to the ionic attraction between the positively charged particles and the negatively charged cell membrane initiates efficient binding and facilitate particle internalization.…”

Section: Influence Of Particle Size and Chargementioning

confidence: 99%

“…In addition, in vitro experiments indicated that internalization of MPG based NPs was carried out through non-endosomal pathway, which confirmed previous reports. 64 In our study, C57BL/6 mice were vaccinated twice (2 weeks interval) with the aforementioned NPs after TC-1 challenge, and the result of cytokine assay indicated that the immune response elicited by MPG based NPs was a dominant Th1 response denoted by the production of IFN-g. 36 …”

Section: Cellular and Nuclear Localization Of Dna Vaccinesmentioning

confidence: 99%

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Multifunctional nanoparticles for cancer immunotherapy

Saleh

Shojaosadati

2016

Human Vaccines & Immunotherapeutics

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During the last decades significant progress has been made in the field of cancer immunotherapy. However, cancer vaccines have not been successful in clinical trials due to poor immunogenicity of antigen, limitations of safety associated with traditional systemic delivery as well as the complex regulation of the immune system in tumor microenvironment. In recent years, nanotechnology-based delivery systems have attracted great interest in the field of immunotherapy since they provide new opportunities to fight the cancer. In particular, for delivery of cancer vaccines, multifunctional nanoparticles present many advantages such as targeted delivery to immune cells, co-delivery of therapeutic agents, reduced adverse outcomes, blocked immune checkpoint molecules, and amplify immune activation via the use of stimuli-responsive or immunostimulatory materials. In this review article, we highlight recent progress and future promise of multifunctional nanoparticles that have been applied to enhance the efficiency of cancer vaccines.

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“…[21] Our research group recently showed that MPG peptide is an efficient delivery system for expression of HPV16 E7 gene in vitro and enhancement of the potency of DNA vaccine expressing E7 in tumor mice model. [45] Generally, there are several peptidebased non-viral vectors known as cell penetrating peptides. Herein, two peptides of Poly-L-lysine (PLL) and GALA are briefly mentioned in below:…”

Section: Lipid-based Non-viral Vectorsmentioning

confidence: 99%

Different strategies of gene delivery for treatment of cancer and other disorders

Agi

Mosaferi

Khatamsaz

et al. 2016

JST

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Gene therapy is the gene transfer into host cells for treatment of acquired and genetic disorders. For this purpose, there are a wide variety of gene delivery methods with special properties including viral and non-viral vectors. The non-viral methods use physical forces or chemical compounds (natural or synthetic) to transfer DNA into a cell. The efficiency of the non-viral gene therapy depends on conquering four different intra-and extra-cellular barriers such as cellular uptake, endosomal escape, nuclear entry, and gene expression. Among various gene carriers, some viral vectors such as Adenovirus, Lentivirus, Vaccinia as well as gene gun and lipofection achieved to clinical trials. In this mini-review, we briefly describe different approaches for gene delivery and their applications in various phases of clinical trials.

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MPG-based nanoparticle: An efficient delivery system for enhancing the potency of DNA vaccine expressing HPV16E7

Cited by 51 publications

References 27 publications

Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

Multifunctional nanoparticles for cancer immunotherapy

Different strategies of gene delivery for treatment of cancer and other disorders

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