MRI and X-ray scanning images of the brain of 3-, 6- and 9-month-old rats with bilateral neonatal ventral hippocampus lesions

Deformation-based brain morphometry in rats

“…In contrast to humans, continuous brain growth in rodents is facilitated by simultaneous expansion of the neurocranium (Sandner et al, 2010). Furthermore, we confirmed results of histological studies that showed rat brain asymmetry (Diamond et al, 1975;Galaburda et al, 1986;Kolb et al, 1982;Rosen et al, 1991Rosen et al, , 1993.…”

Histological correlates of N40 auditory evoked potentials in adult rats after neonatal ventral hippocampal lesion: animal model of schizophrenia

“…In histological studies, NVHL rats show a thin layer of calcium deposits close to the lesioned area, mainly within the auditory thalamus and the zona incerta, in addition to the ventral hippocampus. This abnormality affecting the auditory thalamic region had also been indicated by some other authors, described as a "mild gliosis" in lateral thalamic structures adjacent to the hippocampus (Chambers et al, 1996;Swerdlow et al, 2001;Macedo et al, 2010;Sandner et al, 2010). There are at least three possible causes for these histological findings.…”

Effects of caffeine or RX821002 in rats with a neonatal ventral hippocampal lesion

“…We used the same rats in the three tests. The validity of this method had been previously verified in experiments that we published in the past few years, finding no interference from one test to the subsequent one (Angst et al, 2007; Macedo et al, 2008, 2010, 2012; Sandner et al, 2010, 2011, 2012). …”

“…MRI was performed on a small-animal scanner operating at 4.7 T (TR/TE/TEeff: 3000/30 ms/60 ms). A series of 10 slices (256 × 256 pixels) was generated over a 1 cm long section of the brain, rostral to the cerebellum-cerebrum gap, as in our previous studies and those conducted by others (Angst et al, 2007; Macedo et al, 2008, 2010, 2012; Bertrand et al, 2010; Sandner et al, 2010, 2011, 2012), the purpose being to select triplets of lesioned rats (1 saline, 1 caffeine and 1 RX821002), where each member of the triplet had about the same MRI image in terms of the location and symmetry of the lesion (examples are shown in Figure 1). We obtained 9 triplets of lesions (27 lesioned rats), to which we added 27 sham-lesioned controls.…”

“…Their extension can be assessed before adulthood using magnetic resonance imaging (MRI), namely before experiments are conducted (Angst et al, 2007; Macedo et al, 2008, 2010, 2012; Bertrand et al, 2010; Sandner et al, 2010, 2011, 2012). Selecting rats on the basis of the MRI picture of their lesion provides optimal reproducibility of the experiments.…”