mRNA expression of fibroblast growth factors and hepatocyte growth factor in rat plantaris muscle following denervation and compensatory overload

Yamaguchi, Akihiko; Ishii, Hisayoshi; Morita, Isao; Oota, Isao; Takeda, Hirofumi

doi:10.1007/s00424-004-1282-5

Cited by 25 publications

(27 citation statements)

References 48 publications

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“…1,12,22 Yamaguchi et al 27 addressed the questions of whether hypertrophy induced by compensatory overload differs according to innervation status and how FGF mRNA is expressed using a rat model during overload or denervation. They found that the gene expressions of members in the FGF family differed significantly in response to different stimuli.…”

Section: Discussionmentioning

confidence: 99%

Fibroblast growth factor‐2 enhances functional recovery of reinnervated muscle

Iwata¹,

Ozaki²,

Hirata³

et al. 2006

Muscle and Nerve

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Long-term denervation of muscles results in fibrosis and fat replacement, which prevent muscles from regaining contractile function despite reinnervation. Therefore, prevention of muscle atrophy between nerve repair and muscle reinnervation may improve the functional outcome. A variety of growth factors play significant roles in muscle mass modulation and muscle regeneration. The purpose of the present study was to investigate the effect of fibroblast growth factor-2 (FGF2) and nerve growth factor (NGF) on muscle mass modulation after denervation and reinnervation using a nerve-to-muscle neurotization model. Growth factors were injected into the anterior tibial muscle after direct neurotization of muscles every 7 days up to 4 weeks after surgery. FGF2 significantly increased the amplitude of compound muscle action potentials (CMAPs), wet muscle weight, and the number of motor endplates, especially at higher concentration, compared to the vehicle. In contrast, NGF did not increase either the amplitude of CMAPs or muscle weight, although it significantly increased the number of motor endplates. These findings indicate that both growth factors enhance reinnervation of muscles; however, only FGF2 is involved in muscle mass modulation.

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Section: Discussionmentioning

confidence: 99%

Fibroblast growth factor‐2 enhances functional recovery of reinnervated muscle

Iwata¹,

Ozaki²,

Hirata³

et al. 2006

Muscle and Nerve

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show abstract

“…Previous studies have reported that HGF is present in the extracellular matrix s u r r o u n d i n g m u s c l e f i b e r s 1 6 , 4 8 ) , o r immunolocalized at regenerating skeletal muscle 49) . In situ hybridization has also revealed HGF mRNA in regenerating muscle fibers 15,31) . In the present study, HGF was clearly immunolocalized in the cytoplasm of satellite cells early in the recovery process, strongly suggesting that HGF is produced by local satellite cells in response to reloading stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…Although some studies have examined the role of HGF during maturation and regeneration of skeletal muscle, few attempts have been made to examine changes in HGF expression in hypertrophied and atrophied muscle in vivo. Only the content of HGF mRNA expression following denervation and compensatory overload 31) , and of HGF protein following skeletal muscle injury 32) , have been reported. These reports did not examine changes in HGF protein content following changes in muscle activities.…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

et al. 2006

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Abstract. Hepatocyte growth factor (HGF) has been suggested as a mitogen for skeletal muscle satellite cells and participates in skeletal muscle hypertrophy. The present study assessed HGF levels in mouse soleus and plantaris muscles during 14 days of tail suspension and 3 days of reloading using the enzymelinked immunosorbent assay. Immunohistochemical analyses were used to determine the locations of HGF, its receptor (c-Met) and proliferating cell nuclear antigen. In normal mice, HGF contents were 4.4 ± 0.5 ng/g tissue in the soleus muscle and 5.9 ± 1.2 ng/g tissue in the plantaris muscle, significantly higher than in the soleus muscle. HGF level in the soleus muscle was increased 314% from normal by reloading. HGF and c-Met were expressed in small cells contiguous to muscle fibers. Cells in similar positions displayed reactivity for PCNA, suggesting that these represent activated satellite cells. Thus, production of HGF protein appears to stimulated in satellite cells during recovery from disuse atrophy.

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“…Mitchell et al 11) noted that stretch-induced hypertrophy led to an increase in bFGF mRNA levels in avian muscles and that bFGF localized the perimysium and endomysium of the myofiber periphery. Yamaguchi et al 21) reported that the expression of bFGF mRNAs increased shortly after the initiation of compensatory overload and decreased consequent to denervation. Kästner et al 22) described the enhancement of satellite cell proliferation upon the introduction of bFGF to cultured rat myofibers.…”

Section: Discussionmentioning

confidence: 99%

Continuous Therapeutic Ultrasound Inhibits Progression of Disuse Atrophy in Rat Gastrocnemius Muscles

et al. 2012

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Abstract. [Purpose] This study examined the effects of therapeutic ultrasound on the inhibition of disuse atrophy progression in rat gastrocnemius muscles. [Subjects and Methods] Male Wistar rats were randomly divided into control, ultrasound (US) and sham treatment groups. In the US and sham group, bilateral ankle joints of each rat were fixed in full plantar flexion with a plaster cast over a 2-week period. Continuous ultrasonic energy (frequency, 1 MHz; intensity, 1.0 W/cm 2 ) was delivered to the gastrocnemius muscle of the US group over a 2-week immobilization period.[Results] In the US and sham groups, type IIa and IIb mean muscle fiber diameters decreased significantly relative to the control group. In the US group, muscle fiber diameters of all fiber types were markedly larger than those of the sham group. Heat shock protein 70 (HSP70) content of the US group increased significantly in comparison with the sham group. The levels of basic fibroblast growth factor were substantially higher in the US group relative to the control and sham groups. However, meaningful differences in insulin-like growth factor I level were not evident between the groups. [Conclusion] Continuous therapeutic ultrasound inhibits the progression of disuse muscle atrophy via elevation of HSP70 levels in skeletal muscle.

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mRNA expression of fibroblast growth factors and hepatocyte growth factor in rat plantaris muscle following denervation and compensatory overload

Cited by 25 publications

References 48 publications

Fibroblast growth factor‐2 enhances functional recovery of reinnervated muscle

Fibroblast growth factor‐2 enhances functional recovery of reinnervated muscle

Hepatocyte Growth Factor in Mouse Soleus Muscle Increases with Reloading after Unloading

Continuous Therapeutic Ultrasound Inhibits Progression of Disuse Atrophy in Rat Gastrocnemius Muscles

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