mRNA Expression on Neuropeptide Y (NPY) to Exercise Intensity and Recovery Time

Abstract

Abstract.[Purpose] The purpose of this study was to analyze the effect of exercise intensity and recovery times on the extension of Neuropeptide Y in mice hypothalamus.[Subjects] The mice (ICR) were housed per cage in a temperature (20-23°C) and light controlled environment with a 12:12h light-dark cycle.[Methods] ICR were divided into ten groups, 8 mice each group: rest time group, low-intensity exercise, moderate-intensity and high-intensity. The exercise was carried out on a treadmill adapted in order to permit 2 animals to run at the same. Neuropeptide Y transcript contents were estimated by RT-PCR and Real Time PCR. DNA laddering was used to detect DNA fragmentation as an indicator of Neuropeptide Y.[Results] The Neuropeptide Y mRNA relationship to exercise intensity showed significant differences immediately after exercise. The Neuropeptide Y mRNA relationship to exercise intensity showed differences on low-intensity, moderate-intensity and high-intensity. Neuropeptide Y mRNA showed differences between exercise levels of low-intensity to moderate-intensity and low-intensity to highintensity. [Conclusion] It is noteworthy that the expression of NPY mRNA can be effected by stability time and the intensity of exercise. Neuropeptide Y mRNA expression is connected with recovery times at moderate-intensity and high-intensity. The present results suggest the possibility that exercise intensity and recovery times regulated of the desire for food.

Impact of different running distances on muscle and lymphocyte DNA damage in amateur marathon runners

“…However, Mastaloudis et al 8 ) reported that a high level of DNA damage caused by a 50 km ultramarathon race returned to the resting level 2 hours after the race. It has been reported that the extent and duration of DNA damage can vary according to the type, duration, running distance, and intensity of exercise, as well as training conditions and physical strength 29 , 30 , 31 , 32 , 33 ) . In the present study, the relatively fast recovery from DNA damage is presumed to be due to all subjects in the 3 groups, who were all amateur runners, getting regular aerobic exercise and being familiar with long-distance running, as they had participated in marathon races.…”

Association between gene polymorphisms and obesity and physical fitness in Korean children

“…Neuropeptide Y (NPY) regulates appetite, and thus influences the development of obesity. NPY was shown to be released after intense physical exercise [ 26 ], and neuropeptide Y mRNA expression was related to recovery times after moderate-intensity and high-intensity exercise [ 27 ]. Buemann et al [ 28 ] found that a polymorphism in the UCP2 gene was related to energy consumption-related exercise efficiency during acute exercise, and suggested that the UCP2 gene not only affects basal metabolic rate but also influence energy consumption during exercise.…”

Neuropeptide Y: Biomarker and intervention for surgical recovery

“…An NPY-based screening test to identify surgical resilience pre-operatively would have a large impact in elective surgery allowing for targeted prehabilitation interventions and possibly also in recovery strategies after emergency or trauma surgery. A number of studies have reported behavioural/psychological ( Morgan et al., 2000 ), physical ( Coiro et al., 2011 , Han et al., 2011 ), and dietary interventions ( Huang et al., 2011 ) that increase NPY levels. Beyond screening, interventions may be considered to enhance levels of NPY in a perioperative surgical context.…”