mRNA Relative Expression of Cancer Associated Fibroblasts Markers in Keloid Scars

Sari, Dewi Hambar; Ningsih, Sri; Antarianto, Radiana Dhewayani; Sadikin, Mohamad; Hardiany, Novi Silvia; Jusman, Sri Widia A.

doi:10.1166/asl.2017.9426

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been observed that prolonged treatment with CAP inhibits cell viability and collagen production of murine fibroblasts [93]. A reduction in collagen secretion and the migration behavior was also observed after CAP treatment in keloid fibroblasts, which, like tumor-associated fibroblasts, show an overproduction of collagen [94,95]. Moreover, in vitro studies have shown that CAP is able to destroy collagen [96].…”

Section: Cap Interaction With the Tumor Microenvironmentmentioning

confidence: 99%

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Semmler

Bekeschus

Schäfer

et al. 2020

Cancers

156

127

View full text Add to dashboard Cite

Recently, the potential use of cold atmospheric pressure plasma (CAP) in cancer treatment has gained increasing interest. Especially the enhanced selective killing of tumor cells compared to normal cells has prompted researchers to elucidate the molecular mechanisms for the efficacy of CAP in cancer treatment. This review summarizes the current understanding of how CAP triggers intracellular pathways that induce growth inhibition or cell death. We discuss what factors may contribute to the potential selectivity of CAP towards cancer cells compared to their non-malignant counterparts. Furthermore, the potential of CAP to trigger an immune response is briefly discussed. Finally, this overview demonstrates how these concepts bear first fruits in clinical applications applying CAP treatment in head and neck squamous cell cancer as well as actinic keratosis. Although significant progress towards understanding the underlying mechanisms regarding the efficacy of CAP in cancer treatment has been made, much still needs to be done with respect to different treatment conditions and comparison of malignant and non-malignant cells of the same cell type and same donor. Furthermore, clinical pilot studies and the assessment of systemic effects will be of tremendous importance towards bringing this innovative technology into clinical practice.

show abstract

Section: Cap Interaction With the Tumor Microenvironmentmentioning

confidence: 99%

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Semmler

Bekeschus

Schäfer

et al. 2020

Cancers

156

127

View full text Add to dashboard Cite

show abstract

“…This outcome may be related to the aforementioned effect of CAP on fibroblasts. Another study showed that CAP inhibited the excessive synthesis of collagen in keloid fibroblasts (KFs) (108), and the activated fibroblasts may have similar characteristics with CAFs (109). An in vitro study on the effect of CAP on dentin surfaces and extracted type I collagen fibrils showed that CAP can destroy the structure of collagen (110).…”

Section: Effects Of Cap On a Cellular Tme Componentsmentioning

confidence: 99%

Therapeutic Effects of Cold Atmospheric Plasma on Solid Tumor

et al. 2022

View full text Add to dashboard Cite

Cancer is a devastating disease, and there is no particularly effective treatment at present. Recently, a new treatment, cold atmospheric plasma (CAP), has been proposed. At present, CAP is confirmed to have selective killing effect on tumor by many studies in vitro and in vivo. A targeted literature search was carried out on the study of cold atmospheric plasma. Through analysis and screening, a narrative review approach was selected to describe therapeutic effects of cold atmospheric plasma on solid tumor. According to the recent studies on plasma, some hypothetical therapeutic schemes of CAP are proposed in this paper. The killing mechanism of CAP on solid tumor is expounded in terms of the selectivity of CAP to tumor, the effects of CAP on cells, tumor microenvironment (TME) and immune system. CAP has many effects on solid tumors, and these effects are dose-dependent. The effects of optimal doses of CAP on solid tumors include killing tumor cells, inhibiting non-malignant cells and ECM in TME, affecting the communication between tumor cells, and inducing immunogenic death of tumor cells. In addition, several promising research directions of CAP are proposed in this review, which provide guidance for future research.

show abstract

“…In vitro studies have shown that plasma can destroy dry and dissolved collagen I molecules due to the oxidation of amino acids and breakage of hydrogen bonds [153], which loosen the collagen structure ( Figure 3b) [154]. In addition, plasma decreased the collagen secretion and cell migration in keloid fibroblasts [155], which similarly to CAFs, overproduce collagen [156]. Using Matrigel (matrix extracted from the Engelbreth-Holm-Swarm mouse sarcoma rich in laminin, collagen IV, entactin, proteoglycans, and growth factors) as a surrogate model in vivo, it was shown that high doses of plasma-derived ROS hindered ECM-cell interactions and decreased bone formation, whereas lower ROS doses promoted chondrocyte differentiation, VEGF production and bone formation [157].…”

Section: Acellular Components Of the Tumour Microenvironmentmentioning

confidence: 99%

Modifying the Tumour Microenvironment: Challenges and Future Perspectives for Anticancer Plasma Treatments

Privat-Maldonado

Bengtson

Razzokov

et al. 2019

Cancers

View full text Add to dashboard Cite

Tumours are complex systems formed by cellular (malignant, immune, and endothelial cells, fibroblasts) and acellular components (extracellular matrix (ECM) constituents and secreted factors). A close interplay between these factors, collectively called the tumour microenvironment, is required to respond appropriately to external cues and to determine the treatment outcome. Cold plasma (here referred as ‘plasma’) is an emerging anticancer technology that generates a unique cocktail of reactive oxygen and nitrogen species to eliminate cancerous cells via multiple mechanisms of action. While plasma is currently regarded as a local therapy, it can also modulate the mechanisms of cell-to-cell and cell-to-ECM communication, which could facilitate the propagation of its effect in tissue and distant sites. However, it is still largely unknown how the physical interactions occurring between cells and/or the ECM in the tumour microenvironment affect the plasma therapy outcome. In this review, we discuss the effect of plasma on cell-to-cell and cell-to-ECM communication in the context of the tumour microenvironment and suggest new avenues of research to advance our knowledge in the field. Furthermore, we revise the relevant state-of-the-art in three-dimensional in vitro models that could be used to analyse cell-to-cell and cell-to-ECM communication and further strengthen our understanding of the effect of plasma in solid tumours.

show abstract

mRNA Relative Expression of Cancer Associated Fibroblasts Markers in Keloid Scars

Cited by 3 publications

References 0 publications

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Therapeutic Effects of Cold Atmospheric Plasma on Solid Tumor

Modifying the Tumour Microenvironment: Challenges and Future Perspectives for Anticancer Plasma Treatments

Contact Info

Product

Resources

About