MRSA diversity and the emergence of LA-MRSA in a large teaching hospital in Slovenia

Kevorkijan, Božena Kotnik; Petrovic, Z.; Kocuvan, Aleksander; Rupnik, Maja

doi:10.1556/030.65.2018.052

Cited by 9 publications

(11 citation statements)

References 34 publications

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“…The strains of CA-MRSA have been extensively studied and found in several animals with which humans have a direct contact, such as pets, cattle, pigs and horses, as well as in wild animals [62,63]. Since 2005, MRSA associated with farm animals started to emerge in the human population [64]. The first case of livestock-associated MRSA (LA-MRSA) was reported in early 1970, in Belgium, in bovine mastitis [65].…”

Section: Methicillin-resistant S Aureusmentioning

confidence: 99%

Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review

et al. 2020

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Staphylococcus aureus is an opportunist pathogen that is responsible for numerous types of infections. S. aureus is known for its ability to easily acquire antibiotic resistance determinants. Methicillin-resistant S. aureus (MRSA) is a leading cause of infections both in humans and animals and is usually associated with a multidrug-resistant profile. MRSA dissemination is increasing due to its capability of establishing new reservoirs and has been found in humans, animals and the environment. Despite the fact that the information on the incidence of MRSA in the environment and, in particular, in wild animals, is scarce, some studies have reported the presence of these strains among wildlife with no direct contact with antibiotics. This shows a possible transmission between species and, consequently, a public health concern. The aim of this review is to better understand the distribution, prevalence and molecular lineages of MRSA in European free-living animals.

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Section: Methicillin-resistant S Aureusmentioning

confidence: 99%

Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review

et al. 2020

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“…The strains were considered as S. aureus relying on mannitol salt agar fermentation, gram stain, morphology, catalase test and coagulase test. MRSA isolates were identified upon their resistance to an oxacillin 6 µl/ml using agar screening method (6). MRSA isolates were further identified based on the presence of the mecA gene using PCR-based screening method.…”

Section: Cultural Biochemical and Molecular Identification Of S Aurmentioning

confidence: 99%

“…The agar screening method was used to investigate the antibiotic susceptibility according to the Clinical Laboratory Standards Institute (CLSI) recommendations (6). The nine different antibiotics from different antimicrobial groups were used in this study that supplied by Bioanalyse (Turkey) ( Table 1).…”

Section: Antibiotic Sensitivity Testmentioning

confidence: 99%

Detection of methicillin-resistant Staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques

Assafi¹,

Hado²,

Abdulrahman³

2020

IJVS

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Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health concern. The purpose of this study was to investigate the prevalence rates of MRSA infection amongst broiler chickens and broilers farm workers. The total samples used in this study were 306. Cloacal swab samples from 231 broilers and nasal swab samples from 75 broilers farm workers were collected from five farms in Duhok city, Iraq. Isolation and identification of MRSA isolates were carried out and the antibiotic susceptibility were screened. Molecular characterization of all isolates was performed by using polymerase chain reaction (PCR) technique to detect the mecA gene. S. aureus was detected among 84% (63/75) of the farms workers samples and among 84.8% (196/231) of the broiler's samples. The S. aureus isolated from farm workers and broilers appeared resistant to oxacillin 28.6% (18/63), and 32.1% (63/196), respectively. MRSA colonization in farm workers and broilers was 24% (18/75) and 27.3% (63/231) respectively. The S. aureus isolates showed the most resistant to chloramphenicol and the least resistant to vancomycin. The results of the PCR assays revealed that 85.7% (12/14) of S. aureus isolates from farm workers and 44.4% (16/36) of S. aureus isolates from broilers were positive for the mecA gene. The direct handling of broilers by farm workers plays the important role for transport the MRSA isolates from broilers to broilers farm workers.

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“…It is also generally accepted that A. baumannii clones do not demonstrate such great and rapid variability as other microorganisms causing hospital epidemics (MRSA, VRE) [12,59,60]. Epidemic outbreaks caused by the same epidemic clone of A. baumannii, which occurred in long intervals (of over several months), had been described before [12,61].…”

Section: Discussionmentioning

confidence: 99%

Investigation of Acinetobacter baumannii Activity in Vascular Surgery Units through Epidemiological Management Based on the Analysis of Antimicrobial Resistance, Biofilm Formation and Genotyping

Szczypta

Talaga-Ćwiertnia

Kielar

et al. 2021

IJERPH

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Background/Objectives: The genus Acinetobacter demonstrates resistance to antibiotics and has been shown to spread in the hospital environment causing epidemic outbreaks among hospitalized patients. The objectives of the present study was to investigate the antibiotic resistance, biofilm formation, and clonality among Acinetobacter baumannii strains. Materials and Methods: The study involved 6 (I Outbreak) and 3 (II Outbreak) A. baumannii strains isolated from patients hospitalized in vascular surgery unit. Results: All tested A. baumannii strains were extensively drug resistant (XDR) and all the isolates were carbapenem-resistant and among them, all carried the blaOXA-51 gene, the blaOXA-24 gene, as well as the blaOXA-23 gene. All of the investigated strains had the ability to form a biofilm, but all of them produced less biofilm than the reference strain. Multi-locus sequence typing (MLST) showed that all strains belonged to the ST2 clone. Pulsed-field gel electrophoresis (PFGE) divided the tested outbreak strains into two clones (A and B). Conclusion: This study shows a nosocomial spread of XDR A. baumannii ST2 having the blaOXA-51 gene, the blaOXA-24 gene, as well as the blaOXA-23 gene, low biofilm formers, that was prevalent in the vascular surgery unit. To identify the current situation of vascular surgery departments targeted epidemiological investigation was needed. Effective implementation of infection control prevented the spread of the epidemic outbreaks.

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MRSA diversity and the emergence of LA-MRSA in a large teaching hospital in Slovenia

Cited by 9 publications

References 34 publications

Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review

Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review

Detection of methicillin-resistant Staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques

Investigation of Acinetobacter baumannii Activity in Vascular Surgery Units through Epidemiological Management Based on the Analysis of Antimicrobial Resistance, Biofilm Formation and Genotyping

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