MSCs transfected with hepatocyte growth factor or vascular endothelial growth factor improve cardiac function in the infarcted porcine heart by increasing angiogenesis and reducing fibrosis

Lu, Fanglin; Zhao, Xianxian; Wu, Jun; Cui, Yong; Mao, Yanjun; Chen, Kebiao; Yuan, Yuan; Gong, Daozhi; Zhao, Xu; Huang, Shengdong

doi:10.1016/j.ijcard.2012.06.052

Cited by 52 publications

(43 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HGF-MSCs exhibit anti-fibrotic properties in experimental models of lung, kidney, heart, skin, and liver fibrosis [40][41][42][43]. In our study, treatment with HGF-UCMSCs significantly reduced collagen deposition in the lamina propria based on M&T staining compared with other groups.…”

Section: Discussionsupporting

confidence: 50%

Hepatocyte Growth Factor Gene-Modified Mesenchymal Stem Cells Augment Sinonasal Wound Healing

Zheng

et al. 2015

Stem Cells and Development

View full text Add to dashboard Cite

This study was designed to investigate the effects of hepatocyte growth factor (HGF) transgenic mesenchymal stem cells (HGF-MSCs) on wound healing in the sinonasal mucosa and nasal epithelial cells (NECs). We also sought to determine whether HGF-MSCs and MSCs can migrate into the injured mucosa and differentiate into ciliated cells. Human HGF-overexpressing umbilical cord MSCs (hHGF-UCMSCs) were established, and upregulation of hHGF expression was confirmed by real-time PCR (RT-PCR) and enzyme-linked immunosorbant assay (ELISA). To investigate the paracrine effect of human MSCs (hMSCs) on nasal epithelial repair, hMSC-and HGF-MSC-conditioned media (CM) were used in NEC proliferation assays and in an in vitro scratch-wound repair model. The in vivo sinonasal wound-healing model was established, and all enrolled rabbits were randomly assigned to four groups: the GFP-MSC group, the HGF-MSC group, the Ad-HGF group, and the surgery control group. The average decreased diameter was recorded, and the medial wall of the maxillary sinus was removed for histological analysis and scanning electron microscopy. Collagen deposition in the wound tissue was detected via Masson trichrome (M&T) staining. The distribution of MSCs and HGFMSCs was observed by immunofluorescence. MSCs improved nasal wound healing both in vivo and in vitro. HGF overexpression in MSCs augmented the curative effects. Reduced collagen deposition and transforming growth factor beta1 (TGF-b1) expression were detected in the HGF-MSC group compared with the MSC-, Ad-HGF-, and phosphate-buffered saline-treated groups based on M&T staining and ELISA. The enhanced therapeutic effects of HGF-MSCs were accompanied by decreased level of the fibrogenic cytokine TGF-b1. In addition, both HGF-MSCs and MSCs can migrate to the injured mucosa and epithelial layer.

show abstract

Section: Discussionsupporting

confidence: 50%

Hepatocyte Growth Factor Gene-Modified Mesenchymal Stem Cells Augment Sinonasal Wound Healing

Zheng

et al. 2015

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…20 Several strategies have been developed to accelerate angiogenesis and thus overcome the strict diffusion limits imposed inside bioengineered tissues that are thicker than 500 mm. Such strategies include the provision of various proangiogenic factors, 21,22 genetic modification of cells to overproduce growth factors, 23,24 specific biomaterial design to mimic the extracellular matrix with high porosities, 25 and embedding ECs into engineered tissue constructs. 26,27 However, the time required for EC migration, angiogenic sprouting, and vasculogenesis is still too long to guarantee the viability of bioengineered tissue after implantation and for it to merge and fuse with host tissue.…”

Section: Discussionmentioning

confidence: 99%

Coculture of Stem Cells from Apical Papilla and Human Umbilical Vein Endothelial Cell Under Hypoxia Increases the Formation of Three-Dimensional Vessel-Like Structuresin Vitro

Yuan

Wang²,

Zhu

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

“…[35][36][37][38] Lees et al 35 conducted a meta-analysis of preclinical data for stem cell therapy for experimental stroke (119 studies, 2704 animals). In contrast to our analysis, differences in effect size between cell sources are observed.…”

Section: Noncardiac Meta-analyses Of Stem Cell Therapymentioning

confidence: 99%

Similar Effect of Autologous and Allogeneic Cell Therapy for Ischemic Heart Disease

Eding

Vesterinen

Spoel

et al. 2015

Circulation Research

View full text Add to dashboard Cite

Rationale:In regenerative therapy for ischemic heart disease, use of both autologous and allogeneic stem cells has been investigated. Autologous cell can be applied without immunosuppression, but availability is restricted, and cells have been exposed to risk factors and aging. Allogeneic cell therapy enables preoperative production of potent cell lines and immediate availability of cell products, allowing off-the-shelf therapy. It is unknown which cell source is preferred with regard to improving cardiac function.Objective: We performed a meta-analysis of preclinical data of cell therapy for ischemic heart disease. Methods and Results:We conducted a systematic literature search to identify publications describing controlled preclinical trials of unmodified stem cell therapy in large animal models of myocardial ischemia. Data from 82 studies involving 1415 animals showed a significant improvement in mean left ventricular ejection fraction in treated compared with control animals (8.3%, 95% confidence interval, 7.1-9.5; P<0.001). Meta-regression revealed a similar difference in left ventricular ejection fraction in autologous (8.8%, 95% confidence interval, 7.3-10.3; n=981) and allogeneic (7.3%, 95% confidence interval, 4.4-10.2, n=331; P=0.3) cell therapies. Conclusions: Autologous and allogeneic cell therapy for ischemic heart disease show a similar improvement in

show abstract

MSCs transfected with hepatocyte growth factor or vascular endothelial growth factor improve cardiac function in the infarcted porcine heart by increasing angiogenesis and reducing fibrosis

Cited by 52 publications

References 36 publications

Hepatocyte Growth Factor Gene-Modified Mesenchymal Stem Cells Augment Sinonasal Wound Healing

Hepatocyte Growth Factor Gene-Modified Mesenchymal Stem Cells Augment Sinonasal Wound Healing

Coculture of Stem Cells from Apical Papilla and Human Umbilical Vein Endothelial Cell Under Hypoxia Increases the Formation of Three-Dimensional Vessel-Like Structuresin Vitro

Similar Effect of Autologous and Allogeneic Cell Therapy for Ischemic Heart Disease

Contact Info

Product

Resources

About