MSD2, an apoplastic Mn-SOD, contributes to root skotomorphogenic growth by modulating ROS distribution in Arabidopsis

Chen, Huize; Lee, Jinsu; Lee, Jungmin; Han, Minsoo; Emonet, Aurélia; Lee, Jiyoun; Jia, Xingtian; Lee, Yuree

doi:10.1016/j.plantsci.2022.111192

Cited by 24 publications

(28 citation statements)

References 56 publications

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“…The results might be attributed to the stimulation of antioxidant enzyme activity of K. paniculata within the threshold of Pb stress, but as the stress concentration increased, the enzyme activity system of K. paniculata was disrupted, and the enzyme activity decreased. Similar to previous reports [ 52 ], SOD activity maintained an upward trend to remove the harmful effects of reactive groups in the plant. In conclusion, K. paniculata reduces the toxicity of peroxides mainly by increasing leaf SOD activity and maintaining CAT and POD activity.…”

Section: Discussionsupporting

confidence: 90%

Lead Responses and Tolerance Mechanisms of Koelreuteria paniculata: A Newly Potential Plant for Sustainable Phytoremediation of Pb-Contaminated Soil

Xie

Yao

et al. 2022

IJERPH

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Phytoremediation could be an alternative strategy for lead (Pb) contamination. K. paniculata has been reported as a newly potential plant for sustainable phytoremediation of Pb-contaminated soil. Physiological indexes, enrichment accumulation characteristics, Pb subcellular distribution and microstructure of K. paniculata were carefully studied at different levels of Pb stress (0–1200 mg/L). The results showed that plant growth increased up to 123.8% and 112.7%, relative to the control group when Pb stress was 200 mg/L and 400 mg/L, respectively. However, the average height and biomass of K. paniculata decrease when the Pb stress continues to increase. In all treatment groups, the accumulation of Pb in plant organs showed a trend of root > stem > leaf, and Pb accumulation reached 81.31%~86.69% in the root. Chlorophyll content and chlorophyll a/b showed a rising trend and then fell with increasing Pb stress. Catalase (CAT) and peroxidase (POD) activity showed a positive trend followed by a negative decline, while superoxide dismutase (SOD) activity significantly increased with increasing levels of Pb exposure stress. Transmission electron microscopy (TEM) showed that Pb accumulates in the inactive metabolic regions (cell walls and vesicles) in roots and stems, which may be the main mechanism for plants to reduce Pb biotoxicity. Fourier transform infrared spectroscopy (FTIR) showed that Pb stress increased the content of intracellular -OH and -COOH functional groups. Through organic acids, polysaccharides, proteins and other compounds bound to Pb, the adaptation and tolerance of K. paniculata to Pb were enhanced. K. paniculata showed good phytoremediation potential and has broad application prospects for heavy metal-contaminated soil.

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Section: Discussionsupporting

confidence: 90%

Lead Responses and Tolerance Mechanisms of Koelreuteria paniculata: A Newly Potential Plant for Sustainable Phytoremediation of Pb-Contaminated Soil

Xie

Yao

et al. 2022

IJERPH

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“…The interference expression of AtFSD1 and AtFSD2 upregulated the AtCSD2 expression in Arabidopsis [ 23 ]. In this study, the interference expression of StMSD downregulated the expression levels of its own and most StSOD family members to a certain extent and inhibited the disproportionation of O 2 •− to H 2 O 2 during healing in tubers ( Figure 2 and Figure 3 ), which was similar to the result that the downregulated expression of AtMSD1 and AtMSD2 led to a decreased disproportionation ability of O 2 •− in Arabidopsis leaves and roots, respectively [ 24 , 25 ]. It has been reported that CCS is required for the activation of CSD with the participation of O 2 •− [ 26 ].…”

Section: Discussionsupporting

confidence: 76%

Interference Expression of StMSD Inhibited the Deposition of Suberin and Lignin at Wounds of Potato Tubers by Reducing the Production of H2O2

Ren

Jiang

et al. 2022

Antioxidants

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Superoxide dismutase (SOD) actively participates in the wound stress of plants. However, whether StMSD mediates the generation of H2O2 and the deposition of suberin polyphenolic and lignin at potato tuber wounds is elusive. In this study, we developed the StMSD interference expression of potato plants and tubers by Agrobacterium tumefaciens-mediated transformation. The StSOD expression showed a marked downregulation in StMSD-interference tubers, especially StCSD2 and StCSD3. The content of O2•− exhibited a noticeable increase together with the inhibition in H2O2 accumulation. Moreover, the gene expression levels of StPAL (phenylalanine ammonia-lyase) and StC4H (cinnamate-4-hydroxylase) were downregulated in StMSD-interference tubers, and less suberin polyphenolic and lignin depositions at the wounds were observed. Taken together, the interference expression of StMSD can result in less suberin polyphenolic and lignin deposition by inhibiting the disproportionation of O2•− to H2O2 and restraining phenylpropanoid metabolism in tubers.

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“…To explore the molecular mechanism of ZmGnTL in regulating salt stress, WT and ZmGnTL -OX Arabidopsis plants were subjected to 24 h of salt stress to analyze the expression of genes associated with ion transport ( AKT1, NHX1, VP1 , and KUP7 ) ( Hirsch et al., 1998 ; Apse et al., 2003 ; Li et al., 2005 ; Han et al., 2016 ), antioxidation ( APX1 and Mn-SOD ) ( Li et al., 2019 ; Chen et al., 2022 ), and osmotic adjustment ( PDH, P5CS, CMO , and BADH ) ( Peng et al., 1996 ; Strizhov et al., 1997 ; Fitzgerald et al., 2009 ; Luo et al., 2012 ). The expression of AtNHX1 was significantly higher in ZmGnTL -OX lines than that in WT roots and leaves under salt stress, while the expression was similar under normal condition ( Figure 9 ).…”

Section: Resultsmentioning

confidence: 99%

Mining for salt-tolerant genes from halophyte Zoysia matrella using FOX system and functional analysis of ZmGnTL

Zheng

Zong

et al. 2022

Front. Plant Sci.

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Zoysia matrella is a salt-tolerant turfgrass grown in areas with high soil salinity irrigated with effluent water. Previous studies focused on explaining the regulatory mechanism of Z. matrella salt-tolerance at phenotypic and physiological levels. However, the molecular mechanism associated with salt tolerance of Z. matrella remained unclear. In this study, a high-efficient method named FOX (full-length cDNA overexpression) hunting system was used to search for salt-tolerant genes in Z. matrella. Eleven candidate genes, including several known or novel salt-tolerant genes involved in different metabolism pathways, were identified. These genes exhibited inducible expression under salt stress condition. Furthermore, a novel salt-inducible candidate gene ZmGnTL was transformed into Arabidopsis for functional analysis. ZmGnTL improved salt-tolerance through regulating ion homeostasis, reactive oxygen species scavenging, and osmotic adjustment. In summary, we demonstrated that FOX is a reliable system for discovering novel genes relevant to salt tolerance and several candidate genes were identified from Z. matrella that can assist molecular breeding for plant salt-tolerance improvement.

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MSD2, an apoplastic Mn-SOD, contributes to root skotomorphogenic growth by modulating ROS distribution in Arabidopsis

Cited by 24 publications

References 56 publications

Lead Responses and Tolerance Mechanisms of Koelreuteria paniculata: A Newly Potential Plant for Sustainable Phytoremediation of Pb-Contaminated Soil

Lead Responses and Tolerance Mechanisms of Koelreuteria paniculata: A Newly Potential Plant for Sustainable Phytoremediation of Pb-Contaminated Soil

Interference Expression of StMSD Inhibited the Deposition of Suberin and Lignin at Wounds of Potato Tubers by Reducing the Production of H2O2

Mining for salt-tolerant genes from halophyte Zoysia matrella using FOX system and functional analysis of ZmGnTL

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