MsmiR156 affects global gene expression and promotes root regenerative capacity and nitrogen fixation activity in alfalfa

Aung, Banyar; Gao, Ruimin; Gruber, Margaret Y.; Yuan, Zhongshun; Sumarah, Mark W.; Hannoufa, Abdelali

doi:10.1007/s11248-017-0024-3

Cited by 29 publications

(43 citation statements)

References 66 publications

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“…Notably, this phenomenon seems to be evolutionarily conserved. The same positive relationship between miR156 level and rooting capacity has been found in Malus xiaojinensis and Medicago sativa (Aung et al, 2017;Xu et al, 2017). However, the molecular mechanism by which miR156/SPL regulates root regenerative capacity is poorly understood.…”

Section: Introductionmentioning

confidence: 70%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

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Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by woundinduced auxin biosynthesis. As plants age, the root regenerative capacity gradually decreases. How wounding leads to the auxin burst and how age and wound signals collaboratively regulate root regenerative capacity are poorly understood. Here, we show that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis. Mechanistically, we find that a subset of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors including ABSCISIC ACID REPRESSOR1 and ERF109 is rapidly induced by wounding and serves as a proxy for wound signal to induce auxin biosynthesis. In older plants, SPL2/10/11 directly bind to the promoters of AP2/ERFs and attenuates their induction, thereby dampening auxin accumulation at the wound. Our results thus identify AP2/ERFs as a hub for integration of age and wound signal for root regeneration.

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Section: Introductionmentioning

confidence: 70%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

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“…Another study proposed a role of miR156OE and SPL13i in heat stress tolerance since plants carrying these constructs showed increased antioxidant levels (Matthews et al, 2019). As found by NGS, plants possessing miR156OE exhibited broad changes in gene expression, including genes involved in nodulation, root development and phytohormone biosynthesis (Aung et al, 2017). Taking together, miR156 can improve drought or heat stress tolerance in alfalfa, at least partially by silencing SPL13 (Feyissa et al, 2019;Matthews et al, 2019).…”

Section: Resistance To Abiotic Stressmentioning

confidence: 86%

Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa

et al. 2020

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For several decades, researchers are working to develop improved major crops with better adaptability and tolerance to environmental stresses. Forage legumes have been widely spread in the world due to their great ecological and economic values. Abiotic and biotic stresses are main factors limiting legume production, however, alfalfa (Medicago sativa L.) shows relatively high level of tolerance to drought and salt stress. Efforts focused on alfalfa improvements have led to the release of cultivars with new traits of agronomic importance such as high yield, better stress tolerance or forage quality. Alfalfa has very high nutritional value due to its efficient symbiotic association with nitrogenfixing bacteria, while deep root system can help to prevent soil water loss in dry lands. The use of modern biotechnology tools is challenging in alfalfa since full genome, unlike to its close relative barrel medic (Medicago truncatula Gaertn.), was not released yet. Identification, isolation, and improvement of genes involved in abiotic or biotic stress response significantly contributed to the progress of our understanding how crop plants cope with these environmental challenges. In this review, we provide an overview of the progress that has been made in high-throughput sequencing, characterization of genes for abiotic or biotic stress tolerance, gene editing, as well as proteomic and metabolomics techniques bearing biotechnological potential for alfalfa improvement.

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“…In this study, we found that the OsmiR156regulated OsSPL3 and OsSPL12 repress adventitious root development in rice ( Figures 6C and 11). miR156 was reported to affect rooting in several species (Chuck et al, 2007;Zhang et al, 2011;Feng et al, 2016;Aung et al, 2017;Massoumi et al, 2017;Xu et al, 2017). In maize (Zea mays), corngrass1 mutants have elevated miR156 levels and produce more crown roots (Chuck et al, 2007).…”

Section: The Mir156-spl Module Is Probably a Conserved Regulator Of Amentioning

confidence: 99%

OsSPL3, an SBP-Domain Protein, Regulates Crown Root Development in Rice

et al. 2019

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The major root system of cereals consists of crown roots (or adventitious roots), which are important for anchoring plants in the soil and for water and nutrient uptake. However, the molecular basis of crown root formation is largely unknown. Here, we isolated a rice (Oryza sativa) mutant with fewer crown roots, named lower crown root number1 (lcrn1). Map-based cloning revealed that lcrn1 is caused by a mutation of a putative transcription factor-coding gene, O. sativa SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (OsSPL3). We demonstrate that the point mutation in lcrn1 perturbs theO. sativa microRNA156 (OsmiR156)-directed cleavage of OsSPL3 transcripts, resulting in the mutant phenotype. Chromatin immunoprecipitation sequencing assays of OsSPL3 binding sites and RNA sequencing of differentially expressed transcripts in lcrn1 further identified potential direct targets of OsSPL3 in basal nodes, including a MADS-box transcription factor, OsMADS50. OsMADS50-overexpressing plants produced fewer crown roots, phenocopying lcrn1, while knocking out OsMADS50 in the lcrn1 background reversed this phenotype. We also show that OsSPL12, another OsmiR156 target gene, regulates OsMADS50 and crown root development. Taken together, our findings suggest a novel regulatory pathway in which the OsmiR156-OsSPL3/OsSPL12 module directly activates OsMADS50 in the node to regulate crown root development in rice.

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MsmiR156 affects global gene expression and promotes root regenerative capacity and nitrogen fixation activity in alfalfa

Cited by 29 publications

References 66 publications

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa

OsSPL3, an SBP-Domain Protein, Regulates Crown Root Development in Rice

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