Msx1 homeodomain transcription factor and TATA-binding protein interact to repress the expression of the glycoprotein hormone α subunit gene

Park, Ki-Sun; Kim, Kee K.; Kim, Kyoon Eon

doi:10.1016/j.bbrc.2015.10.102

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…A decline in Msx1 expression in a temporal manner relieves this repression allowing production of the gonadotropic hormones (Xie, et al 2013). Another study validated the role of Msx1 as a transcription repressor of the Cga gene and demonstrated that the mechanism by which Msx1 regulates inhibition of Cga gene expression involves its interaction with the TATA-binding protein in thyrotrophs (Park, et al 2015). Other important factors of Cga gene regulation includes Sine oculis-related homeobox 3 (SIX3) homeodomain transcription factors that represses transcription of the alpha subunit in immature gonadotroph cell lines (Xie, et al 2015).…”

Section: Transcriptional Regulation Of α-Glycoprotein Hormone Subumentioning

confidence: 87%

Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

Das

Kumar

2018

Journal of Molecular Endocrinology

116

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Section: Transcriptional Regulation Of α-Glycoprotein Hormone Subumentioning

confidence: 87%

Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

Das

Kumar

2018

Journal of Molecular Endocrinology

116

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“…In contrast, in mouse C2C12 myoblast cells, the interaction of Msx1 with PIAS1 was required for transcriptional repression and inhibition of myoblast differentiation [ 41 ]. Other reported functions of Msx1 homeoprotein include repression of the glycoprotein hormone α subunit gene by interaction of the Msx1 homeodomain and TATA-binding protein [ 42 ]. In contrast, interaction of the C-terminal domain of Msx1 with a heat shock factor led to activation of Hspa1b promoter activity [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Homeoprotein Msx1-PIASy Interaction Inhibits Angiogenesis

Son

Rho

Kim

et al. 2020

Cells

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Previously, we demonstrated that the homeoprotein Msx1 interaction with p53 inhibited tumor growth by inducing apoptosis. However, Msx1 can exert its tumor suppressive effect through the inhibition of angiogenesis since growth of the tumor relies on sufficient blood supply from the existing vessels to provide oxygen and nutrients for tumor growth. We hypothesized that the inhibition of tumor growth by Msx1 might be due to the inhibition of angiogenesis. Here, we explored the role of Msx1 in angiogenesis. Overexpression of Msx1 in HUVECs inhibited angiogenesis, and silencing of Msx1 by siRNA abrogated its anti-angiogenic effects. Furthermore, forced expression of Msx1 in mouse muscle tissue inhibited vessel sprouting, and application of an Ad-Msx1-transfected conditioned medium onto the chicken chorioallantoic membrane (CAM) led to a significant inhibition of new vessel formation. To explore the underlying mechanism of Msx1-mediated angiogenesis, yeast two-hybrid screening was performed, and we identified PIASy (protein inhibitor of activated STAT Y) as a novel Msx1-interacting protein. We mapped the homeodomain of Msx1 and the C-terminal domain of PIASy as respective interacting domains. Consistent with its anti-angiogenic function, overexpression of Msx1 suppressed the reporter activity of VEGF. Interestingly, PIASy stabilized Msx1 protein, whereas deletion of the Msx1-interacting domain in PIASy abrogated the inhibition of tube formation and the stabilization of Msx1 protein. Our findings suggest the functional importance of PIASy-Msx1 interaction in Msx1-mediated angiogenesis inhibition.

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“…En condiciones normales, esta región tiene una alta importancia funcional, ya que se ha reportado su interacción con otras proteínas reguladoras de la transcripción. [10][11][12][13][14][15][16] El estudio de las interacciones proteicas de hTBP a través de su región rica en glutaminas es altamente relevante, ya que el incremento del número de glutaminas puede alterar la función de esta proteína y ocasionar una enfermedad neurodegenerativa llamada ataxia espinocerebelosa 17 (SCA17), caracterizada por síntomas como ataxia, demencia, movimientos coréicos y distonia, epilepsia, señales piramidales y extrapiramidales, discapacidad cognitiva e, incluso, trastornos psiquiátricos. 17 En nuestro laboratorio utilizamos el modelo de la mosca de la fruta para estudiar las interacciones de Antp con TBP en el control genético del desarrollo, por lo que en este trabajo determinamos la interacción entre la TBP humana (hTBP) con las homeoproteínas Sex combs reduced (Scr), Ultrabithorax (Ubx) y Abdominal B (AbdB), mediada por la región rica en glutaminas.…”

Section: Antecedentesunclassified

<i>Drosophila</i> en el estudio de las interacciones proteicas de hTBP en el desarrollo y modelaje de SCA17

Montalvo-Méndez,

Cárdenas-Tueme,

Reséndez-Pérez

2024

GMM

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Antecedentes: Las interacciones proteicas participan en una gran cantidad de mecanismos moleculares que rigen los procesos celulares. La proteína de unión a la caja TATA humana (hTBP) interacciona con Antennapedia (Antp) a través de su extremo N-terminal, específicamente a través de sus homopéptidos de glutaminas. Esta región PolyQ sirve como sitio de unión a factores de transcripción en condiciones normales, pero cuando se expande genera la ataxia espinal cerebelosa 17 (SCA17), cuyos agregados proteicos en el cerebro impiden su funcionamiento correcto. Objetivo: Determinar si la región rica en glutaminas de hTBP interviene en su interacción con homeoproteínas y el papel que tiene en la formación de agregados proteicos en SCA17. Material y métodos: Se caracterizó la interacción de hTBP con otras homeoproteínas usando BiFC y se modeló SCA17 en Drosophila melanogaster dirigiendo hTBPQ80 al cerebro de las moscas usando UAS/GAL4. Resultados: Existió interacción de hTBP con homeoproteínas a través de su región rica en glutaminas. Los agregados proteicos de hTBP con las glutaminas expandidas afectaron la capacidad locomotriz de las moscas. Conclusiones: El estudio de las interacciones de hTBP abre la posibilidad para la búsqueda de nuevas estrategias terapéuticas en patologías neurodegenerativas como SCA17.

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Msx1 homeodomain transcription factor and TATA-binding protein interact to repress the expression of the glycoprotein hormone α subunit gene

Cited by 9 publications

References 18 publications

Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

Molecular regulation of follicle-stimulating hormone synthesis, secretion and action

Homeoprotein Msx1-PIASy Interaction Inhibits Angiogenesis

<i>Drosophila</i> en el estudio de las interacciones proteicas de hTBP en el desarrollo y modelaje de SCA17

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