MTT colorimetric assay for testing macrophage cytotoxic activity in vitro

Ferrari, Marisa Belicchi; Fornasiero, M. C.; Isetta, Anna Maria

doi:10.1016/0022-1759(90)90187-z

Cited by 499 publications

(287 citation statements)

References 9 publications

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“…MTT proliferation assay was carried out to determine the growth rate of cells. A linear relationship between the formazan generated and the number of viable cells was demonstrated, together with time-dependent growth characteristics for HeLa cells (Ferrari et al, 1990). GTN treatment on HeLa cells cell lines showed significant decrease in growth rate compared with control.…”

Section: Discussionmentioning

confidence: 80%

Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

Alabsi

Ali²,

Ali³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 80%

Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

Alabsi

Ali²,

Ali³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…The oil and pentamidine isethionate (reference drug) were added to the culture, and after 24 h the viability of the treated cells was compared to the control without drugs, through the MTT methodology. 17,18 cytotoxicity assay…”

Section: Cultures Of Leishmania Amazonensismentioning

confidence: 99%

“…The sample and pentamidine isethionate (reference drug) were added to the culture, and after 24 h the viability of treated cells was compared to the control without drugs, through the MTT methodology. 17,18 resUlts And discUssion chemical study Hydrodistillation of the leaves of A. foetida gave a yellowish crude essential oil, with a yield of 0.01% in relation to the dry weight of the plant material, and a density at 25 o C of 0.9055 g mL -1 . As shown in Table 1, 34 compounds of the essential oil were identified by GC/FID and GC/MS analysis, comprising 93.06% of the oil.…”

Section: Cultures Of Leishmania Amazonensismentioning

confidence: 99%

Antimicrobial and antileishmanial activity of essential oil from the leaves of Annona foetida (Annonaceae)

Costa¹,

Pinheiro²,

Silva³

et al. 2009

Quím. Nova

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Recebido em 2/1/08; aceito em 6/8/08; publicado na web em 15/12/08The essential oil from leaves of Annona foetida obtained by hydrodistillation was analyzed by GC/FID and GC/MS. The results showed that the major constituents were bicyclogermacrene (35.12%), (E)-caryophyllene (14.19%) and α-copaene (8.19%). The antimicrobial and antileishmanial activities were investigated. The oil showed potent antimicrobial activity against Candida albicans and Rhodococcus equi. The oil also showed significant antileishmanial activity, giving the best results against Leishmania guyanensis. A preliminary cytotoxicity assay for this oil was carried out on hamster and mice (Balb/c) peritoneal macrophages. The results obtained were similar to pentamidine and considered not to be cytotoxic to macrophages.

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“…The cytotoxic activity of Dox was then evaluated using a standard MTT assay. 13 The absorbency at λ = 450nm was determined using a microKinetics Reader BT 2000. Each concentration point was determined from samplings from eight separate wells.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Alteration of Genomic Responses to Doxorubicin and Prevention of MDR in Breast Cancer Cells by a Polymer Excipient: Pluronic P85

Batrakova

Kelly

et al. 2005

Mol. Pharmaceutics

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Polymer therapeutics has emerged as a new clinical option for the treatment of human diseases. However, little is known about pharmacogenetic responses to drugs formulated with polymers. In this study, we demonstrate that a formulation containing the block copolymer Pluronic P85 and antineoplastic drug, doxorubicin (Dox), prevents the development of multidrug resistance in the human breast carcinoma cell line, MCF7. Specifically, MCF7 cells cultured in the presence of Pluronic were unable to stably grow in concentrations of Dox that exceeded 10ng Dox/ml of culture media. In sharp contrast, MCF7 cells cultured in the absence of the block copolymer resulted in the selection and stable growth of cells that tolerated 1000 times higher concentration of the drug (10,000ng Dox/ml culture media). Detailed characterization of the isolated sublines demonstrated that those cells selected in the polymer-drug formulation did not show amplification of the MDR1 gene, likely resulting in their high sensitivity to the drug. Conversely, cells selected with Dox alone showed an elevated level in the expression of the MDR1 gene along with a corresponding increase in the expression level of the drug efflux transporter, Pgp, and likely contributing to the high resistance of the cells to Dox. Global analysis of the expression profiles of 20K genes by DNA microarray revealed that the use of Pluronic in combination with Dox drastically changed the direction and magnitude of the genetic response of the tumor cells to Dox and may potentially enhance therapeutic outcomes. Overall, this study reinforces the need for a thorough assessment of pharmacogenomic effects of polymer therapeutics.

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MTT colorimetric assay for testing macrophage cytotoxic activity in vitro

Cited by 499 publications

References 9 publications

Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

Antimicrobial and antileishmanial activity of essential oil from the leaves of Annona foetida (Annonaceae)

Alteration of Genomic Responses to Doxorubicin and Prevention of MDR in Breast Cancer Cells by a Polymer Excipient: Pluronic P85

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