MTUS1 tumor suppressor and its miRNA regulators in fibroadenoma and breast cancer

Kara, Murat; Kaplan, Mehmet; Bozgeyik, İbrahim; Özcan, Önder; Çelik, Özgür; Bozgeyik, Esra; Yumrutaş, Önder

doi:10.1016/j.gene.2016.05.006

Cited by 21 publications

(14 citation statements)

References 18 publications

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“…7 They play an important role as either tumor suppressor or oncogene by participating in epigenetic modification and regulation of the targeted proteins. 8,9 MiRNAs have the potential to be used as a biomarker in the clinical therapy of human cancers. 10 MiR-30 family are reported to be involved in the progression and invasion of various human cancers.…”

Section: Introductionmentioning

confidence: 99%

Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis

Zhang

Zhao

et al. 2017

Tumour Biol.

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MiR-30 family plays an important role in the tumorigenesis of human cancers. The aim of the study is to investigate the role of miR-30d in human colon cancer cell lines and explore the molecular mechanism in the proliferation of colon cancer cells. The expression of miR-30d was determined by real-time polymerase chain reaction assay in colon cancer cell lines (HCT15, HCT116, HT-29, DLD-1, and SW480) and the results demonstrated that miR-30d level was significantly decreased in human colon cancer cell lines, compared with normal colon epithelial cell line. Transfection with miR-30d mimics inhibited cell proliferation, and transfection with miR-30d inhibitors significantly promoted cell viability of colon cancer cells. Furthermore, TargetScan analysis predicted that miR-30d interacted with messenger RNA on its 3' untranslated region of ATG5, phosphoinositide 3-kinase, and Beclin1 to negatively regulate cell autophagy in colon cancer cells. Moreover, transfection with miR-30d induced cell arrest at G2/M phase of HT-29 cells. Overexpression of miR-30d mimics inhibited cell viability probably due to the inhibition of cell autophagy and promotion of cell apoptosis. Thus, MiR-30d inhibited cell autophagy by directly targeting messenger RNA of ATG5, phosphoinositide 3-kinase, and Beclin1 and promoted cell apoptosis of human colon cancer cells. It is helpful to clarify the function of miR-30d in tumorigenesis of human cancers.

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Section: Introductionmentioning

confidence: 99%

Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis

Zhang

Zhao

et al. 2017

Tumour Biol.

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“…14,15 It is well known that miRNAs play an important role in multiple biological processes, including growth, cell death, central nervous system function, and tumor formation. [16][17][18][19] A number of studies using microRNA microarray analyses in mouse and model cells have shown that expression of the miR-34 family increases with age. 20 Recent studies have shown that miR-34 overexpression accelerates the aging process of human diploid cells 21 and endothelial progenitor cells.…”

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confidence: 99%

MiR-34c Participates in Diabetic Corneal Neuropathy Via Regulation of Autophagy

Kan

2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To investigate the contribution and mechanism of miRNAs and autophagy in diabetic peripheral neuropathy. METHODS. In this study, we used streptozotocin (STZ)-induced type I diabetes C57 mice as animal models, and we detected the expression of miR-34c and autophagic intensity in trigeminal ganglion (TG) tissue. The bioinformatics software was used to predict and analyze the potential targets of miR-34c. Primary trigeminal ganglion neurons were cultured in vitro to investigate the effect of miR-34c on axon growth and survival of TG cells. A corneal epithelial damage-healing model was established on the diabetic mice, then miR-34c antagomir was injected subconjunctivally. The condition of corneal epithelial healing was observed through sodium fluorescein staining, and the peripheral nerve degeneration of the cornea was evaluated by b-tublin corneal nerve staining. RESULTS. The expression of miR-34c was significantly increased in TG tissue of type I diabetic mice by real-time PCR. Western blot showed that autophagy-related proteins Atg4B and LC3-II were significantly down-regulated in diabetes TG compared with normal control. Trigeminal neuron immunofluorescence showed that the length of the trigeminal ganglion cell synapses was significantly increased after miR-34c antagomir treatment compared with normal cultures. Subconjunctival injection of miR-34c antagomir can significantly promote corneal epithelium healing of diabetic mice and appreciably promote the regeneration of corneal nerve. At the same time, it can significantly increase the expression of autophagy in TG tissue of type I diabetic mice. CONCLUSIONS. In this study , miR-34c was found to affect the growth of trigeminal sensory neurons and the repair of diabetic corneal nerve endings by acting directly on Atg4B.

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“…Nevertheless, GBC remains aggressive, needing new biomarkers. MTUS1 is a tumor-suppressor gene as was shown in several studies (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Thus, the expression of MTUS1 protein and mRNA in tumor tissues was significantly lower than that in normal tissues of several human tumor types, including breast cancer, gastric carcinoma, head and neck squamous cell carcinoma, salivary adenoid cystic carcinoma, and urinary bladder cancer (8,11,13,16,17).…”

Section: Kaplan-meier Analysis For Cancer-specific (A) and Disease-mentioning

confidence: 87%

Loss of MTUS1 Expression Is Associated With Poor Prognosis in Patients With Gallbladder Carcinoma

Sim¹,

Kim²,

Kim³

et al. 2019

In Vivo

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Background/Aim: Microtubule-associated scaffold protein 1 (MTUS1) acts as tumor suppressor in several cancer types. This study assessed the relationship between clinicopathological characteristics and expression of microRNA candidates based on MTUS1 expression in gallbladder cancer (GBC). Materials and Methods: MTUS1 expression was evaluated by immunohistochemical staining of tissue microarrays from 109 cases of GBC. The association of MTUS1 expression with clinicopathological factors was explored. Two microRNA candidates (miR-19a-3p, and miR-19b-3p), which were identified by a literature review and computational analysis, were assessed in GBC tissue samples by quantitative real-time polymerase chain reaction. Results: Low MTUS1 expression in GBC was associated with high histological grade, perineural invasion, lymphovascular invasion, high T-stage, advanced TNM stage, poorer disease-free survival, and poorer cancer-specific survival. No statistical association between MTUS1 expression and expression of microRNA candidates was observed. Conclusion: MTUS1 may act as tumor suppressor and might be a potential biomarker for predicting prognosis in GBC. Although gallbladder cancer (GBC) is rare, it is the most common malignant tumor of the biliary tract (1-4). No specific symptoms or reliable diagnostic markers are established for GBC. In addition, GBC is diagnosed late; moreover, systemic chemotherapy and radiation therapy do not produce significant improvement in survival or quality of life for patients with GBC (1-3). Thus, patients with GBC do not have a favorable clinical outcome (1-3). Therefore, it is essential to evaluate new biomarkers for predicting the prognosis of patients with GBC. Microtubule-associated scaffold protein 1 (MTUS1), also known as angiotensin II receptor-interacting protein (ATIP), is located on chromosome 8p22 and contains 17 exons (5, 6). MTUS1 acts as a tumor-suppressor gene, exhibiting low expression in a variety of malignancies, including breast, colorectal, head and neck, ovary, stomach, salivary gland, and urinary bladder cancer (7-18). MicroRNAs (miRNAs) are short, noncoding RNAs that regulate gene expression by binding target mRNA at the post-transcriptional level. As miRNA pairing targets mRNAs in an imperfect manner, a single miRNA can target multiple genes; and one gene may be affected by multiple miRNAs (1, 19, 20). Recently, Ge et al. demonstrated that miR-19a and miR-19b co-regulate MTUS1 in lung cancer (14). Kara et al. reported decreased MTUS1 expression and elevated miR-183-5p expression in advanced stages of breast cancer (10). Additionally, several miRNAs including miR-135b-5p, miR-373-3p, miR-183-5p, miR-142-5p, miR-200c-3p, and miR-19a-3p, may play important roles in colorectal carcinoma through MTUS1 (21). The role of MTUS1 as a tumor suppressor has been reported, however, the expression of MTUS1 and its clinicopathological significance in GBC has not yet been evaluated. Thus, this study aimed to assess the clinicopathological parameters in association with MTUS1 expr...

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MTUS1 tumor suppressor and its miRNA regulators in fibroadenoma and breast cancer

Cited by 21 publications

References 18 publications

Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis

Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis

MiR-34c Participates in Diabetic Corneal Neuropathy Via Regulation of Autophagy

Loss of MTUS1 Expression Is Associated With Poor Prognosis in Patients With Gallbladder Carcinoma

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