Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples

Abstract

Five cold-adapted bacteria belonging to the genus Mucilaginibacter were isolated from lichen and soil samples collected from Finnish Lapland and investigated in detail by phenotypic and phylogenetic analyses. Based on 16S rRNA gene phylogeny, the novel strains represent three new branches within the genus Mucilaginibacter. The strains were aerobic, chemo-organotrophic, non-motile rods and formed pigmented, smooth, mucoid colonies on solid media. The strains grew between 0 and 33 °C (optimum growth at 25 °C) and at pH 4.5–8.0 (optimum growth at pH 6.0). The main cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH) and iso-C17 : 0 3-OH and the major respiratory quinone was MK-7. The DNA G+C contents were 44.0–46.5 mol%. Based on phylogenetic, phenotypic and chemotaxonomic data, the strains represent three novel species of the genus Mucilaginibacter for which the names Mucilaginibacter frigoritolerans sp. nov. (type strain FT22T =ATCC BAA-1854T =LMG 25359T), Mucilaginibacter lappiensis sp. nov. (type strain ANJLI2T =ATCC BAA-1855T =LMG 25358T) and Mucilaginibacter mallensis sp. nov. (type strain MP1X4T =ATCC BAA-1856T =LMG 25360T) are proposed.

Endophytic bacterial communities in three arctic plants from low arctic fell tundra are cold-adapted and host-plant specific

“…For example, Mucilaginibacter sp. isolate M1Ju16 from J. trifidus was closely related to M. frigoritolerans FT22 that was isolated from Kilpisjärvi soil after experimental freeze‐thaw treatment (Männistö et al ., ). Mucilaginibacter and the closely related Pedobacter spp.…”

Microbial community structure associated with treatment of azo dye in a start-up anaerobic sequenced batch reactor

“…Band J showed 87% sequence similarity to Elusimicrobium, which could grow heterotrophically on sugars and ferment glucose into acetate, ethanol and hydrogen [40,41]. Band R showed a 72% sequence similarity to a Mucilaginibacter clone (within the Sphingobacteriaceae family) identified from soil and lichen samples [42]. However, the clones were not the main strains responsible for azo-reduction in the latter enrichment culture.…”

Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost

“…The 16S rRNA gene sequences of the strain TR6-03 T determined in this study were continuous stretches of 1480 bp Differentiating characteristics of M. composti TR6-03 T and the type strains of related Mucilaginibacter species Strains: 1, Mucilaginibacter composti TR6-03 T ; 2, Mucilaginibacter lappiensis ANJLI2 T ; 3, Mucilaginibacter rigui WPCB133 T ; 4, Mucilaginibacter dorajii FR-f4 T Data were from this study, Männistö et al (2010), Baik et al (2010), and Kim et al (2010). All strains were Gram-negative, non-motile, rod shaped, positive for oxidase activities.…”