Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis

Han, Song-Ih; Lee, Hyo Jin; Lee, Hae-Ran; Kim, Ki-Kwang; Whang, Kyung-Sook

doi:10.1099/ijs.0.029793-0

Cited by 42 publications

(27 citation statements)

References 23 publications

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“…; Han et al . ). Mucilaginibacter are also known to produce large amounts of extracellular polysaccharides containing the sugars glucose, galactose, mannose and rhamnose and may thus provide a carbon source for Saccharomyces (Urai et al .…”

Section: Discussionmentioning

confidence: 97%

“…Mucilaginibacter is a member in the family Sphingobacteriaceae, and different members of this genus have the ability to hydrolyse organic matter such as xylan, pectin and laminarin (Pankratov et al 2007;Madhaiyan et al 2010;Han et al 2012). Mucilaginibacter are also known to produce large amounts of extracellular polysaccharides containing the sugars glucose, galactose, mannose and rhamnose and may thus provide a carbon source for Saccharomyces (Urai et al 2008).…”

Section: Yeast Growth On Oak Barkmentioning

confidence: 99%

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The interaction of Saccharomyces paradoxus with its natural competitors on oak bark

2015

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The natural history of the model yeast Saccharomyces cerevisiae is poorly understood and confounded by domestication. In nature, S. cerevisiae and its undomesticated relative S. paradoxus are usually found on the bark of oak trees, a habitat very different from wine or other human fermentations. It is unclear whether the oak trees are really the primary habitat for wild yeast, or whether this apparent association is due to biased sampling. We use culturing and high-throughput environmental sequencing to show that S. paradoxus is a very rare member of the oak bark microbial community. We find that S. paradoxus can grow well on sterile medium made from oak bark, but that its growth is strongly suppressed when the other members of the community are present. We purified a set of twelve common fungal and bacterial species from the oak bark community and tested how each affected the growth of S. paradoxus in direct competition on oak bark medium at summer and winter temperatures, identifying both positive and negative interactions. One Pseudomonas species produces a diffusible toxin that suppresses S. paradoxus as effectively as either the whole set of twelve species together or the complete community present in nonsterilized oak medium. Conversely, one of the twelve species, Mucilaginibacter sp., had the opposite effect and promoted S. paradoxus growth at low temperatures. We conclude that, in its natural oak tree habitat, S. paradoxus is a rare species whose success depends on the much more abundant microbial species surrounding it.

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Section: Discussionmentioning

confidence: 97%

Section: Yeast Growth On Oak Barkmentioning

confidence: 99%

The interaction of Saccharomyces paradoxus with its natural competitors on oak bark

2015

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“…Psychrobacter ibarius has been found in Dendrobium o cinale. Some researchers have isolated this bacterium from the root plane of Angelica sinensis [60]. This is a fungus related to polysaccharide synthesis.…”

Section: Differences Of Bacterial Communities In Rhizosphere Soils Ofmentioning

confidence: 99%

Bacterial Composition and Diversity Associated with Rhizosphere of the Chinese Medicinal Herb Dendrobium

Yuan

Liu

et al. 2020

Preprint

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Background: Dendrobium is a precious herbal belongs to Orchid and widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most researches still focus on endophytes, and there is still large unknown in rhizosphere microorganisms. In order to investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during maturity stage of three kinds of Dendrobium species.Results: In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, Gemmatimonadetes. We analyzed the environmental factors of the growth of Dendrobium and found that the environmental factor that affects the rhizosphere soil microorganisms of Dendrobium is the soil factor. Among them, soil factors most closely related to the influence of Dendrobium rhizosphere soil microorganisms include total nitrogen, available phosphorus, ammonium nitrogen and pH value.Conclusions: We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, Bacteroidetes. Moreover, the smaller the level of bacterial, the greater the difference among Dendrobium species. Soil is the most important environmental factor affecting the bacterial communities in the rhizosphere soil of Dendrobium. These results fill the gap in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.

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“…The type species of the genus Mucilaginibacter is M. paludis (Pankratov et al , 2007). Strains of the genus Mucilaginibacter have been isolated from acidic peat [ M. gracilis and M. paludis (Pankratov et al , 2007)], compost [ M. composti (Cui et al , 2011)], freshwater [ M. rigui (Baik et al , 2010); M. myungsuensis (Joung & Joh, 2011); M. soyangensis (Joung et al , 2014a)], lichen [ M. lappiensis (Männistö et al , 2010)], marine sand [ M. litoreus (Yoon et al , 2012); M. sabulilitoris (Kang et al , 2013)], plant stem [ M. auburnensis (Kämpfer et al , 2014)], the plant rhizosphere or soil [ M. kameinonensis (Urai et al , 2008); M. ximonensis (Luo et al , 2009); M. oryzae (Jeon et al , 2009); M. dorajii (Kim et al , 2010); M gossypii and M. gossypiicola (Madhaiyan et al , 2010); M. frigoritolerans and M. mallensis (Männistö et al , 2010); M. boryungensis (Kang et al , 2011); M. angelicae (Kim et al , 2012a); M. polysacchareus (Han et al , 2012); …”

mentioning

confidence: 99%

Mucilaginibacter gotjawali sp. nov., isolated from soil of a lava forest

Lee

Kim

Eom

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, designated SA3-7 T , was isolated from soil of a lava forest located in Jeju, Republic of Korea. Cells of strain SA3-7 T were Gram-stain-negative, oxidase-and catalasepositive, non-motile rods and produced creamy white colonies on ten-fold-diluted R2A agar. The isolate contained menaquinone-7 (MK-7) as the predominant isoprenoid quinone and summed feature 3 (C 16 : 1 v7c/C 16 : 1 v6c), iso-C 15 : 0 and iso-C 17 : 0 3-OH as the major fatty acids. The DNA G+C content was 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain SA3-7 T was related most closely to Mucilaginibacter frigoritolerans FT22 T (96.7 % sequence similarity) and that it formed a separate lineage in the genus Mucilaginibacter. Combined phenotypic, chemotaxonomic and phylogenetic characteristics supported the conclusion that strain SA3-7 T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter gotjawali sp. nov. is proposed; the type strain is SA3-7 T (5KCTC 32515 T 5CECT 8628 T 5DSM 29289 T ).The genus Mucilaginibacter is a member of the family Sphingobacteriaceae within the phylum Bacteroidetes. It was proposed by Pankratov et al. (2007) with two species, Mucilaginibacter paludis and Mucilaginibacter gracilis, isolated from an acidic Sphagnum peat bog. At the time of writing, the genus Mucilaginibacter contains 30 species with validly published names (http://www.bacterio.net/mucilaginibacter. html; Euzéby, 1997), members of which are Gram-stainnegative, non-motile, non-spore-forming, rod-shaped bacteria that have menaquinone-7 (MK-7) as the predominant isoprenoid quinone. The type species of the genus Mucilaginibacter is M. paludis Strain SA3-7 T was recovered during an investigation of the bacterial diversity of soil of the Gotjawal forest, Jeju, Korea. Soil samples were diluted serially in sterile distilled water and samples of each serial dilution were spread on onetenth-strength R2A agar (BD) and incubated at 25 u C for 4 weeks. A pinpoint colony, designated SA3-7 T , was isolated, purified by subculturing on the same medium and maintained as glycerol/water suspensions (20 %, v/v) at 270 u C. For most experiments, strain SA3-7 T was cultivated on ten-fold-diluted R2A agar at 25 uC for 5 days, unless stated otherwise. The cultivation of strain SA3-7 T and reference strains were tested on R2A agar, one-tenthstrength R2A agar, trypticase soy agar (TSA; BD), one-tenth-strength TSA, nutrient agar (NA; BD) andThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Mucilaginibacter gotjawali SA3-7 T is KM588182.

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Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis

Cited by 42 publications

References 23 publications

The interaction of Saccharomyces paradoxus with its natural competitors on oak bark

The interaction of Saccharomyces paradoxus with its natural competitors on oak bark

Bacterial Composition and Diversity Associated with Rhizosphere of the Chinese Medicinal Herb Dendrobium

Mucilaginibacter gotjawali sp. nov., isolated from soil of a lava forest

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