Mucinous Secretion of Totally Isolated Porcine Stomach Perfused with Homologous Blood

Abstract

Principal glycoproteins and amino acids were determined in porcine gastric juice obtained from totally isolated stomachs perfused extracorporeally with homologous blood. Acid juices collected during prolonged stimulation of stomachs with histamine or pentagastrin, infused into gastric arteries, were used. All samples showed the presence of galactose, glucosamine, galactosamine, fucose, mannose and sulphate. Galactose and glucosamine were found in equimolar amounts and the galactose to galactosamine ratio was, in most samples, 2:1. Compared with human non-stimulated gastric juice studied by others, marked differences were found in the relationship between carbohydrate components and between amino acids. Porcine gastric juice contained much more protein than human gastric juice. Some characteristics of porcine mucinous secretion may be due to conditions of juice collection from the isolated gastric preparation.

Effect of Ischemic Anoxia on Electrical and Mechanical Activity of the Totally Isolated Porcine Stomach

“…For both stomach dissection and perfusion, pigs were anesthetized with a mixture of halothane and air. The method of anesthesia, difficult in this species, was reported previously [12,13].…”

“…The perfusion system used for the study of ex vivo stomachs was described previously [12,13] and we will only give a few pertinent facts in this paper. Figure 1 summarizes this information schematically.…”

“…The purpose of dissection was to mobilize the whole stomach, keeping all gastric vascular arcades intact. The steps of dissection were previously described [12,13].…”

“…Changes in blood gases and pH can suddenly occur and, if not corrected, they will induce rapid deterioration of the per fusion organ. This subject was discussed previously [12,13,19]. Methods of biochemical analysis and the tables with 'normal values' for the porcine perfusion blood were also reported [11][12][13][15][16][17],…”

Mucinous Secretion from Canine Heidenhain Pouch after Stimulation with Food, Pentagastrin and Histamine

“…Gastric mucin contains high mo lecular weight glycoproteins, mucopolysaccharides, blood group sub stances, peptic enzymes and various proteins. Some components of gastric mucin have been studied previously in both in vivo [1,2,5,6,8,9,14,15] and ex vivo conditions [3], The purpose of the present experiment was to analyze canine gastric secretion for various mucinous components and for amino acids. Gastric secretion was obtained from canine Heiden hain pouches after stimulation with food, histamine or pentagastrin.…”