Mucins as a New Frontier in Pulmonary Fibrosis

Ballester, Beatriz; Milara, Javier; Cortijo, Julio

doi:10.3390/jcm8091447

Cited by 41 publications

(39 citation statements)

References 185 publications

(239 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 MUC1 has been studied in many diseases, such as tumor, respiratory diseases, and inflammatory bowel disease, and its vital role in the pathogenesis of these diseases has been demonstrated. 6,8 However, there is no report on the role of MUC1 in NEC so far. Our study showed that the expression of MUC1 was dramatically decreased in the intestinal tissue of NEC patients.…”

Section: Discussionmentioning

confidence: 99%

Mucins and Tight Junctions are Severely Altered in Necrotizing Enterocolitis Neonates

Liu

Feng

et al. 2020

Am J Perinatol

View full text Add to dashboard Cite

Objective This study investigates the expression levels of mucin 1 (MUC1), MUC2, occludin, and zonula occludens-1 (ZO-1) in necrotizing enterocolitis (NEC). Study Design Intestinal specimens of surgical patients suffering from NEC (the NEC group) and intestinal specimens of patients with congenital intestinal atresia (the control group) were collected. Immunohistochemical changes in MUC1, MUC2, occludin, and ZO-1 were compared between the two groups. Results Our study showed a significant decrease in the expression levels of MUC1 (p = 0.004), MUC2 (p = 0.001), occludin (p = 0.004), and ZO-1 (p = 0.013) in neonates suffering from NEC as compared with the control group. Conclusion Mucins and tight junctions are severely altered in NEC neonates. This finding might provide clues for rupture of the intestinal barrier. Further research is needed to investigate the gene expression as well as the exact mechanisms behind these changes. This will help us better understand the role of the intestinal barrier in NEC. Key Points

show abstract

Section: Discussionmentioning

confidence: 99%

Mucins and Tight Junctions are Severely Altered in Necrotizing Enterocolitis Neonates

Liu

Feng

et al. 2020

Am J Perinatol

View full text Add to dashboard Cite

show abstract

“…Gel‐forming mucin glycoproteins form the major components of mucus and are the main products of goblet cells. Mucins are divided into two subtypes: cell surface‐tethered/membrane‐bound mucins (MUC1, MUC3A/B, MUC4, MUC12‐17 and MUC20‐22) and secreted mucins 21 . Secreted mucins are further divided into two subfamilies, gel‐forming (MUC2, MUC5AC, MUC5B, MUC6 and MUC19) and non–gel‐forming (MUC7‐9) mucins, which maintain the structure and organization of the mucous gel and control its viscoelastic properties 21 .…”

Section: The Mucous Barriermentioning

confidence: 99%

“…Mucins are divided into two subtypes: cell surface‐tethered/membrane‐bound mucins (MUC1, MUC3A/B, MUC4, MUC12‐17 and MUC20‐22) and secreted mucins 21 . Secreted mucins are further divided into two subfamilies, gel‐forming (MUC2, MUC5AC, MUC5B, MUC6 and MUC19) and non–gel‐forming (MUC7‐9) mucins, which maintain the structure and organization of the mucous gel and control its viscoelastic properties 21 . Cell‐tethered mucins form a gel‐like layer around cilia of the respiratory epithelium that aid normal ciliary action of moving mucus in mucociliary clearance mechanisms 4 .…”

Section: The Mucous Barriermentioning

confidence: 99%

Impact of diet and the bacterial microbiome on the mucous barrier and immune disorders

Alemao

Budden

Gomez

et al. 2020

Allergy

View full text Add to dashboard Cite

The prevalence of chronic immune and metabolic disorders is increasing rapidly. In particular, inflammatory bowel diseases, obesity, diabetes, asthma and chronic obstructive pulmonary disease have become major healthcare and economic burdens worldwide. Recent advances in microbiome research have led to significant discoveries of associative links between alterations in the microbiome and health, as well as these chronic supposedly noncommunicable, immune/metabolic disorders. Importantly, the interplay between diet, microbiome and the mucous barrier in these diseases has gained significant attention. Diet modulates the mucous barrier via alterations in gut microbiota, resulting in either disease onset/exacerbation due to a “poor” diet or protection against disease with a “healthy” diet. In addition, many mucosa‐associated disorders possess a specific gut microbiome fingerprint associated with the composition of the mucous barrier, which is further influenced by host‐microbiome and inter‐microbial interactions, dietary choices, microbe immigration and antimicrobials. Our review focuses on the interactions of diet (macronutrients and micronutrients), gut microbiota and mucous barriers (gastrointestinal and respiratory tract) and their importance in the onset and/or progression of major immune/metabolic disorders. We also highlight the key mechanisms that could be targeted therapeutically to prevent and/or treat these disorders.

show abstract

“…We also observed that 7118 TGAKIKLVGT 7127 derived from MUC19 reduced the levels of TNF-α, IL-1β and IL-6 in H 2 O 2 -treated A549 cells and the degree of decrease is related to the concentration of peptide as shown in Figure 5. As a secreted mucin MUC19, is released to the extracellular medium and has been identi ed in respiratory, digestive, and reproductive tracts [73]. It has been reported that MUC19 was differentially regulated after exposure to in ammatory cytokines [74].…”

Section: Discussionmentioning

confidence: 99%

Peptidome Analysis of Umbilical Cord Mesenchymal Stem Cell (hUC-MSC) Conditioned Medium from Preterm and Term Infants

Wang

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The therapeutic role of mesenchymal stem cells (MSCs) has been widely confirmed in several animal models of premature infant diseases. Micromolecule peptides have shown promise for the treatment of premature infant diseases. However, the potential role of peptides secreted from MSCs has not been studied. The purpose of this study is to helps to broaden the knowledge of the hUC-MSC secretome at the peptide level through peptidomic profile analysis.Methods: We used tandem mass tag (TMT) labeling technology followed by tandem mass spectrometry to compare the peptidomic profile of preterm and term umbilical cord MSC (hUC-MSC) conditioned medium (CM). Gene Ontology (GO) enrichment analysis and Ingenuity Pathway Analysis (IPA) were conducted to explore the differentially expressed peptides by predicting the functions of their precursor proteins. To evaluate the effect of candidate peptides on human lung epithelial cells stimulated by hydrogen peroxide (H2O2), quantitative real-time PCR (qRT-PCR), western blot analysis and enzyme-linked immunosorbent assay (ELISA) were respectively adopted to detect inflammatory cytokines (TNF-α, IL-1β and IL-6) expression levels at the mRNA and protein levels,Results: A total of 131 peptides derived from 106 precursor proteins were differentially expressed in the preterm hUC-MSC CM compared with the term group, comprising 37 up-regulated peptides and 94 down-regulated peptides. Bioinformatics analysis showed that these differentially expressed peptides may be associated with developmental disorders, inflammatory response and organismal injury. We also found that peptides 7118TGAKIKLVGT7127 derived from MUC19 and 508AAAAGPANVH517 derived from SIX5 reduced the expression levels of TNF-α, IL-1β and IL-6 in H2O2-treated human lung epithelial cells.Conclusions: In summary, this study provides further secretomics information on hUC-MSCs and provides a series of peptides that might have antiinflammatory effects on pulmonary epithelial cells and contribute to the prevention and treatment of respiratory diseases in premature infants.

show abstract

Mucins as a New Frontier in Pulmonary Fibrosis

Cited by 41 publications

References 185 publications

Mucins and Tight Junctions are Severely Altered in Necrotizing Enterocolitis Neonates

Mucins and Tight Junctions are Severely Altered in Necrotizing Enterocolitis Neonates

Impact of diet and the bacterial microbiome on the mucous barrier and immune disorders

Peptidome Analysis of Umbilical Cord Mesenchymal Stem Cell (hUC-MSC) Conditioned Medium from Preterm and Term Infants

Contact Info

Product

Resources

About