Mucoadhesive cationic lipid-based Flavobacterium oreochromis nanoencapsulation enhanced the efficacy of mucoadhesive immersion vaccination against columnaris disease and strengthened immunity in Asian sea bass (Lates calcarifer)

Bunnoy, Anurak; Thangsunan, Patcharapong; Chockmamgmepisarn, Putita; Yata, Teerapong; Klongklaew, Nawanith; Pirarat, Nopadon; Kitiyodom, Sirikorn; Srisapoome, Prapansak; Rodkhum, Channarong

doi:10.1016/j.fsi.2022.06.059

Cited by 23 publications

(15 citation statements)

References 28 publications

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“…Furthermore, this vaccine effectively upregulates several immune-related genes, such as IgM , IgD , IgT , MHCIIα , TCRα , and CD4 , which are key components of specific immune systems. This information is firmly in line with previous studies [ 12 , 14 , 28 , 41 , 42 , 43 ], suggesting that the bivalent nanovaccines in the current study are potent immunogens that vigorously drive the two arms of both the innate and adaptive immune systems [ 12 ]. Particularly for IgM and IgT , almost all vaccinated treatments showed very high upregulation, indicating that two immersion and one booster vaccination of the bivalent nanovaccines effectively enhance both systemic and local specific immune responses.…”

Section: Discussionsupporting

confidence: 92%

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Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms

Meachasompop,

Bunnoy,

Keaswejjareansuk

et al. 2023

Vaccines

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In the present study, chitosan-based bivalent nanovaccines of S. iniae and F. covae were administered by immersion vaccination at 30 and 40 days after hatching (DAH), and the third vaccination was orally administered by feeding at 50 DAH. ELISA revealed that the levels of total IgM and specific IgM to S. iniae and F. covae were significantly elevated in all vaccinated groups at 10, 20, and 30 days after vaccination (DAV). A qRT-PCR analysis of immune-related genes revealed significantly higher IgT expression in the vaccinated groups compared to the control group, as revealed by 44–100-fold changes in the vaccinated groups compared to the control (p < 0.001) at every tested time point after vaccination. All vaccinated groups expressed IgM, MHCIIα, and TCRα at significantly higher levels than the control group at 10 and/or 20 DAV (p < 0.05). In the S. iniae challenge tests, the survival of vaccinated groups ranged from 62.15 ± 2.11 to 75.70 ± 3.36%, which significantly differed from that of the control group (44.44 ± 1.92%). Similarly, all vaccinated groups showed higher survival rates of 68.89 ± 3.85 to 77.78 ± 5.09% during F. covae challenge than the control groups (50.00 ± 3.33%) (p < 0.05).

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Section: Discussionsupporting

confidence: 92%

“…These methods were previously found to exhibit low efficacy in protection but lately have been developed to meet an acceptable level important for industry. With the progress and development of nanotechnology, nanovaccines with mono- and bivalent characteristics have been effectively developed [ 12 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms

Meachasompop,

Bunnoy,

Keaswejjareansuk

et al. 2023

Vaccines

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“…Examples are booster vaccination, administration of immunostimulants/adjuvants, use of delivery vehicles, preconditioning by physical methods, use of novel live attenuated vaccines and DNA vaccines, etc ( 269 – 271 ). Mucoadhesive polymers are widely used in drug delivery and can also be developed and utilized as vaccine carriers in immersion immunization ( 272 – 274 ).…”

Section: Aquatic Vaccination Methodsmentioning

confidence: 99%

Current status and development prospects of aquatic vaccines

Liu

et al. 2022

Front. Immunol.

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Diseases are a significant impediment to aquaculture’s sustainable and healthy growth. The aquaculture industry is suffering significant financial losses as a result of the worsening water quality and increasing frequency of aquatic disease outbreaks caused by the expansion of aquaculture. Drug control, immunoprophylaxis, ecologically integrated control, etc. are the principal control strategies for fish infections. For a long time, the prevention and control of aquatic diseases have mainly relied on the use of various antibiotics and chemical drugs. However, long-term use of chemical inputs not only increases pathogenic bacteria resistance but also damages the fish and aquaculture environments, resulting in drug residues in aquatic products, severely impeding the development of the aquaculture industry. The development and use of aquatic vaccines are the safest and most effective ways to prevent aquatic animal diseases and preserve the health and sustainability of aquaculture. To give references for the development and implementation of aquatic vaccines, this study reviews the development history, types, inoculation techniques, mechanisms of action, development prospects, and challenges encountered with aquatic vaccines.

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“…It is an economically important catadromous species in the Indo-Pacific region 17 L. calcarifer has great economic worth in Thailand as an export, but since the fish is farmed across the country, the disposal of fish scale waste in landfills has become problematic due to space limitations. 18 Le et al 19 reported that the processing of L. calcarifer for export and domestic consumption produces a significant amount of waste, estimated at 50% of the starting material by weight. This amount of waste will present a challenge to the environment if not handled carefully.…”

Section: Introductionmentioning

confidence: 99%

“…The white seabass ( L. calcarifer ) is classified as a saltwater and brackish water fish with large ctenoid scales. It is an economically important catadromous species in the Indo‐Pacific region 17 L. calcarifer has great economic worth in Thailand as an export, but since the fish is farmed across the country, the disposal of fish scale waste in landfills has become problematic due to space limitations 18 . Le et al 19 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of hydroxyapatite from recycled fish scale and its application as a filler in a biodegradable food tray

Kongthong,

Trongnit,

Sriwoon

et al. 2023

Int J Applied Ceramic Tech

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Fish scale waste can be used as a precursor of hydroxyapatite (HAp). In this work, HAp was extracted from white seabass (Lates calcarifer) scale waste by using an alkali heat treatment. The fish scale was pretreated with hydrochloric acid (HCl) concentrations of 0.1, 0.5, and 1 M. The pretreated fish scale was then treated with 2% sodium hydroxide (NaOH) solution at 70°C for 5 h and again (20% NaOH solution) for 1 h to extract HAp. The effects of each pretreatment on the properties of the extracted HAp were investigated. HAp produced after pretreatment with 1 M HCl had the smallest particle size (28.24 ± 5.20 μm), the highest degree of crystallinity (79%), the highest thermal stability, the largest BET surface area (90.80 m2/g), and the highest whiteness value (97.360 ± 0.01). 1 M HCl could eliminate more protein, collagen, and other organic compounds than lower HCl concentrations without destroying the crystalline domains of the fish scale. Waste L. calcarifer scale is therefore a potential source of HAp. Furthermore, in a preliminary study for further research, HAp extracted in the proposed procedure was used as a filler in a biodegradable starch foam.

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Mucoadhesive cationic lipid-based Flavobacterium oreochromis nanoencapsulation enhanced the efficacy of mucoadhesive immersion vaccination against columnaris disease and strengthened immunity in Asian sea bass (Lates calcarifer)

Cited by 23 publications

References 28 publications

Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms

Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms

Current status and development prospects of aquatic vaccines

Characterization of hydroxyapatite from recycled fish scale and its application as a filler in a biodegradable food tray

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