A series of new phthalimides compounds [3][4][5][6][7] a-i were synthesized from reaction of Malic anhydride, phthalic anhydride, nitro phthalic anhydride, 2-phenyl-4H-benzo [d][1,3]oxazin-4-one, 2-(4-nitrophenyl)-4H-benzo [d][1,3]oxazin-4-one with different amino acids as glycine, alanine, valine, leucine, isoleucine, serine, threonine, tyrosine and Phenyl alanine [1] a-i under fusion conditions. Compounds [3-7] a-i react with SOCl 2 in the presence of benzene to produce compounds [8-12] a-i . Chemical modification of Poly(vinyl alcohol)were obtained by reaction of PVA with compounds [8-12] a-i using the dimethyl formamide to give compounds [13-17] a-i . The structure of the synthesized compounds was characterized by their analytical and spectral data as, IR spectra, 1 H, 13 C-NMR, Elemental analysis (CHN), UV-Vis Spectroscopy, Scanning electron microscopy (SEM), Antibacterial activity were screened via two kinds of bacteria. Also, anticancer activity were examined for most of the modified polyvinyl alcohol.