Mucoadhesive nanostructured polyelectrolytes complexes modulate the intestinal permeability of methotrexate

Boni, Fernanda Isadora; Almeida, Andreia; Lechanteur, Anna; Sarmento, Bruno; Cury, Beatriz Stringhetti Ferreira; Gremião, Maria Palmira Daflon

doi:10.1016/j.ejps.2017.09.042

Cited by 51 publications

(20 citation statements)

References 65 publications

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“…The band at 1,630 cm ‐1 is related to a primary amide (López‐Pérez et al., 2007). Bands at 2,856 and 3,247 cm ‐1 are related to the vibration of CH 2 and the O‐H bonds (Boni et al., 2018; Doshi et al., 2018; Jamal et al., 2018; Liu et al., 2019). The enlargement of the O‐H band for samples CG10, CG20, and CG30 suggests the increase of the amount of hydroxyl group due to adding glycerol according to Martínez‐Camacho et al.…”

Section: Resultsmentioning

confidence: 99%

Effect of glutaraldehyde/glycerol ratios on the properties of chitosan films

Oliveira

Ugucioni

Borges

2020

J Food Process Preserv

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Chitosan is a biodegradable polymer and could be applied as packaging. To make chitosan more competitive, plasticizers and cross‐linking agents are added in films. The aim of this paper was to obtain and characterize films of chitosan without or with glutaraldehyde (0; 0.99 mg/150 ml) in different concentrations of glycerol (0, 10, 20, and 30%w/w), for improving the properties like tensile resistance and the water vapor permeability. The films were homogeneous, without phase separation, and with a similar chemical structure. The glutaraldehyde makes the chitosan partly hydrophobic, leading to glycerol saturation in samples with glutaraldehyde and 20 or 30% of glycerol. The Principal Component Analysis for the film with glutaraldehyde and 10% of glycerol presented 3.60 g.mm/kPa.day.m2 of water vapor permeability and 36.96 MPa of tensile resistance. The use of plasticizer and cross‐linking in combination is promising to make chitosan film more competitive on the market of packaging. Practical Applications The addition of glutaraldehyde and glycerol was efficient to improve film properties like water vapor permeability and mechanical properties. The chitosan films with glycerol and glutaraldehyde exhibited similar physical and chemical properties to non‐biodegradable polymers from the petrochemical origin and non‐renewable sources. Furthermore, chitosan films with these additives can be applied as packaging, improving the competitiveness of this biopolymer to the market. The technology described in the paper is important for application as secondary or tertiary packaging in the food industry.

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Section: Resultsmentioning

confidence: 99%

Effect of glutaraldehyde/glycerol ratios on the properties of chitosan films

Oliveira

Ugucioni

Borges

2020

J Food Process Preserv

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“…Polymeric nanoparticles (PN) were developed according to the polyelectrolytic complexation methodology, using a cationic charge polymer (chitosan (CS)) and an anionic charge polymer (HPMC) according to Boni et al [30], CS was dispersed in 0.1 mol L −1 acetic acid, while HPMC was dispersed in distilled water obtaining in both, a final concentration of 2 mg mL −1 . The pH of the solutions were obtained with the aid of a pHmeter Mylabor (OA210A) brand and the adjustment was performed by dripping an alkaline solution of NaOH 1M, to obtain a final pH of 5.5.…”

Section: Development Of Polymeric Nanoparticlesmentioning

confidence: 99%

“…To obtain PN by polyelectrolytic complexation, Boni et al [30] used the positive charge polymers chitosan and the negative charge, hyaluronic acid, and HPMC. The PN obtained with and without API showed sizes ranging between 325.7 and 450.5, and zeta potential ranging between ±20.9 and ±33.1, which is similar to the results found in the formulations named PN-1, PN-2, PN-3, and PN-4.…”

Section: Dynamic Light Scattering Zeta Potential and Experimental Dmentioning

confidence: 99%

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

et al. 2020

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Bacterial resistance has become an important public health problem. Bacteria have been acquiring mechanisms to resist the action of antimicrobial active pharmaceutical ingredients (API). Based on this, a promising alternative is the use of nanotechnology, since when the systems are presented in nanometric size, there is an increase in the interaction and concentration of the action at the target site improving the activity. Thus, this study aims to develop a polymeric nanoparticle (PN) composed of chitosan and hydroxypropylmethylcellulose, as an innovative strategy for the administration of an association between ceftriaxone and extract of S. brasiliensis, for the treatment of Enterobacteriaceae. From a Box–Behnken design, nanoparticles were obtained and evaluated using the DLS technique, obtaining the particle size between 440 and 1660 nm, IPD from 0.42 to 0.92, and positive charges. Morphological characteristics of PN by SEM revealed spherical morphology and sizes similar to DLS. Infrared spectroscopy showed no chemical interaction between the components of the formulation. The broth microdilution technique evaluated their antimicrobial activity, and a considerable improvement in the activity of the extract and the API compared to the free compounds was found, reaching an improvement of 133 times in the minimum inhibitory activity CRO.

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“…Generally, different materials and systems are able to establish supramolecular interactions with mucosal components, such as mucin chains, because of the presence of reactive functional groups at the polymer chains, which facilitate adhesion to the mucus. On the other hand, to design mucus penetrating systems, the opposite logic must be used in order to open paths, facilitating system permeation [19,[25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Exploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery

Ferreira

Alonso

Kiill

et al. 2018

European Polymer Journal

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Monoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. The mucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drug delivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hypothesis is that by combining these opposing functions, this system could have its properties modulated according to specific purposes. Self-assembly studies were conducted using three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoparticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nanoparticles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-polycation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic constraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different behaviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively charged particles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers.

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Mucoadhesive nanostructured polyelectrolytes complexes modulate the intestinal permeability of methotrexate

Cited by 51 publications

References 65 publications

Effect of glutaraldehyde/glycerol ratios on the properties of chitosan films

Effect of glutaraldehyde/glycerol ratios on the properties of chitosan films

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

Exploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery

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