Mucoadhesive properties of carbopol or chitosan-coated liposomes and their effectiveness in the oral administration of calcitonin to rats

Takeuchi, Hirofumi; Matsui, Yuji; Yamamoto, Hiromitsu; Kawashima, Yasunaru

doi:10.1016/s0168-3659(02)00411-x

Cited by 267 publications

(127 citation statements)

References 13 publications

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“…Particles larger than 200 nm in diameter did not penetrate as much as 80 nm Iiposomes. Additionally , it was also found that the amount of liposomal particles penetrating the mucous layer increased when the size of liposomes was reduced to 100 nm [26]. Moreover, Ho et a/.…”

Section: Discussionmentioning

confidence: 93%

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

et al. 2006

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The influence of docosahexaenoic acid (DHA)-and eicosapentaenoic acid (EPA)-enriched phosphatidylcholine (PC) on the permeability, transport and uptake of phospholipids was evaluated in Caco-2 cells . The cells were grown on permeable polycarbonate transwell filters, thus allowing separate access to the apical and basolateral chambers . The monolayers of the cells were used to measure lucifer yellow permeability and transepithelial electrical resistance Our results show that DHA-and EPA-enriched PC increases tight junction permeability across the Caco-2 cell monolayer whereas soy PC has no effect on tight junction permeability .Transportation and uptake of DHA-and EPA-enriched PC SUV differed significantly (P<0.01) from those of soy PC SUV at all doses. We found that PC SUV transported across Caco-2 monolayer and was taken up by Caco-2 cells with very slight injury of the cell membrane up to 100 /-1M PC. Lactate dehydrogenase release and cell viability did not differ significantly between the treatment and control, emphasizing that injury was minimal. Our results suggest that DHA-and EPA-enriched PC enhance the permeability, transport and uptake of PC SUV across monolayers of Caco-2 cells.

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Section: Discussionmentioning

confidence: 93%

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

et al. 2006

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“…It is reported that bLF entered into the blood circulation through the lymphatic pathway after oral administration. 34 L-bLF transported through the blood circulation may locally affect periodontal tissue cells. In addition, L-bLF in drinking water can also penetrate directly into periodontal tissue through JE and act on its receptors on tissue-resident cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis

Yamano

Miyauchi

Furusyo

et al. 2010

Laboratory Investigation

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Bovine lactoferrin (bLF) modulates the production of proinflammatory cytokines including tumor necrosis factor (TNF)-a, and may thus control alveolar bone destruction associated with periodontitis. In this study, the effects of bLF on mRNA expression in lipopolysaccharide (LPS)-stimulated osteoblasts (OBs) and on LPS-induced osteoclastogenesis were examined. The inhibitory effects of oral administration of liposomal-bLF (L-bLF), which improved the robustness of bLF to digestive enzymes, on alveolar bone resorption using LPS-induced periodontitis rat model are also reported. Three groups of 7-week-old male Wistar rats were treated with L-bLF (L-bLF group), bLF (bLF group), or the vehicle (control group) in drinking water (n ¼ 6 in each group). On day 7, LPS was topically applied into the gingival sulcus. Number of osteoclasts and immunoexpression of TNF-a were analyzed. The bLF inhibited the upregulation of TNF-a-mRNA-and upregulation of receptor activator of NFkB (RANKL)-mRNA expression and eliminated downregulation of osteoprotegerin (OPG)-mRNA expression in LPS-stimulated OBs and reduced LPS-induced osteoclastogenesis in co-culture with primary OBs and bone marrow cells. In the control group, the number of osteoclasts increased after LPS treatment. The number of osteoclasts that appeared along the alveolar bone margin was significantly reduced (Po0.01) in the L-bLF but not in the bLF group. Furthermore, L-bLF suppressed upregulation of TNF-a immunoexpression in periodontal tissue and TNF-a and interleukin (IL)-1b-mRNA level in gingival tissue. The results of this study indicate that oral administration of L-bLF significantly reduces alveolar bone resorption induced by LPS stimulation through inhibition of TNF-a production and modulation of RANKL/OPG balance in OBs. It is suggested that L-bLF could be a potent therapeutic and preventive agent for attenuating alveolar bone destruction in periodontitis patients.

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“…The mucoadhesive formulations, administered by rectal (Choi, Oh, and Kim 1998;Ryu et al 1999;Yahagi, Onishi, and Machida 1999), vaginal (Lee and Chien 1996;Valenta et al 2001;Ceschel et al 2001a;Cevher, Sahin, and Araman 2002;Chang et al 2002;Chien and Lee 2002;El-Kamel et al 2002;Maggi et al 2000), oral (Takeuchi et al 2003), buccal (Shojaei, Zhou, and Li 1998;Alur et al 1999;Han et al 1999;Choi and Kim 2000;Shin, Bum, and Choi 2000;Ceschel, Maffei, and Lombardi 2001b;Perioli et al 2004), nasal (Ugwoke et al 1999;Yahagi et al 2000;Pavelic, SkalkoBasnet, and Schubert, 2001;Hasçiçek, Gönül, and Erk 2003;58 E. CEVHER ET AL. Sales et al 2003), and ocular (Yun et al 1999;Ceulemans et al 2001) routes, have been prepared by using polymers showing appropriate adhesiveness.…”

Section: Human Papilloma Virus (Hpv) Causes Infection In the Genital mentioning

confidence: 99%

Evaluation of Mechanical and Mucoadhesive Properties of Clomiphene Citrate Gel Formulations Containing Carbomers and Their Thiolated Derivatives

Cevher

Taha²,

Orlu

et al. 2008

Drug Delivery

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Mucoadhesive properties of carbopol or chitosan-coated liposomes and their effectiveness in the oral administration of calcitonin to rats

Cited by 267 publications

References 13 publications

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis

Evaluation of Mechanical and Mucoadhesive Properties of Clomiphene Citrate Gel Formulations Containing Carbomers and Their Thiolated Derivatives

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