Mucus secretions from a conditionally reprogrammed primary endocervical cell culture

Park, Daye; Reddy, Ashok P.; Wilmarth, Phillip A.; Jensen, Jeffrey T.; Han, Leo

doi:10.1016/j.xfss.2022.03.003

Cited by 2 publications

(2 citation statements)

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“…RNA-Sequencing Data Analysis. 13,784 genes from Rhesus Macaque endocervical cell cultures were included in differential expression analysis after low count filtering. Our study identified 158 genes that show significant differential expression in E2-only conditions compared to hormone-free control, and 250 genes that show significant differential expression in P4-treated conditions compared to E2-only conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The CRCs utilized in this experiment have been shown to expand and maintain steroid receptor expression and secrete mucus analogous to in vivo mucus, and therefore provide an ideal model for mechanistic studies of cervical mucus regulation. 12,13 To gain a better understanding of the molecular basis of mucus changes throughout the menstrual cycle, we performed RNA-sequencing (RNA-seq) on our cultured endocervical cells. From that data set, our study specifically explores the hormonal regulation of pathways and genes involved in mucin synthesis, mucus hydration via ion channel regulation, mucus cross-linking and stabilization, protein-and enzyme-mediated mucus modification, motile ciliogenesis, and hormonal signaling (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional profiling of mucus production and modification in rhesus macaque endocervical cells under hormonal regulation

Rapp,

Wei,

Roberts

et al. 2023

Preprint

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Objective: Endocervical mucus production is a key regulator of fertility throughout the menstrual cycle. With cycle-dependent variability in mucus quality and quantity, cervical mucus can either facilitate or block sperm ascension into the upper female reproductive tract. This study seeks to identify genes involved in the hormonal regulation of mucus production, modification, and regulation through profiling the transcriptome of endocervical cells from the non-human primate, the Rhesus Macaque (Macaca mulatta). Design: Experimental. Setting: Translational science laboratory. Intervention: We treated differentiated primary endocervical cultures with estradiol (E2) and progesterone (P4) to mimic peri-ovulatory and luteal-phase hormonal changes. Using RNA-sequencing, we identified differential expression of gene pathways and mucus producing and modifying genes in cells treated with E2 compared to hormone-free conditions and E2 compared to E2-primed cells treated with P4. Main Outcome Measures: We pursued differential gene expression analysis on RNA-sequenced cells. Sequence validation was done using qPCR. Results: Our study identified 158 genes that show significant differential expression in E2-only conditions compared to hormone-free control, and 250 genes that show significant differential expression in P4-treated conditions compared to E2-only conditions. From this list, we found hormone-induced changes in transcriptional profiles for genes across several classes of mucus production, including ion channels and enzymes involved in post-translational mucin modification that have not previously been described as hormonally regulated. Conclusion: Our study is the first to use an in vitro culture system to create an epithelial-cell specific transcriptome of the endocervix. As a result, our study identifies new genes and pathways that are altered by sex-steroids in cervical mucus production.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%