Mullet and gudgeon liver histopathology and macroinvertebrate indexes and metrics upstream and downstream from a wastewater treatment plant (Febros River—Portugal)

Silva

2022

Water

The concentrations of seventeen endocrine disruptor compounds (EDCs) that included oestrogens, phytoestrogens, sitosterol, and banned industrial pollutants were investigated at ten sites of the Douro River estuary. Surface waters were collected during 2019. After evaluating the physicochemical data (ammonia, nitrates, nitrites and phosphates), the waters were filtrated and submitted to solid-phase extraction (SPE) to extract and pre-concentrate (4000-fold) the EDCs. The extracts were derivatized with BSTFA + 1% TMS and analysed by gas chromatography-mass spectrometry (GC-MS). All EDCs showed a high detection rate (97%, on average), exhibiting ubiquity in this estuary. The finding of biologically relevant amounts of oestrogens (up to 8.5 ng/L for oestradiol, E2), phytoestrogens (up to 827 ng/L for biochanin A, BIO-A) and industrial pollutants (up to 2.7 µg/L for nonylphenol di-ethoxylated, NP2EO) strongly support ongoing risks of endocrine disruption for the local aquatic wildlife. Globally, there was an E2-equivalents (E2-EQs) concentration of 25 ng/L and a hazard index (HI) of 26, which further indicates considerable potential for adverse effects on local biota. Moreover, the physicochemical data suggest direct sewage discharges. Beyond possible toxicological effects on fauna, the detected contaminants may pose risks to humans via direct contact (bathing at local fluvial beaches) or by ingestion (local fish).

Section: Industrial Compoundsmentioning

confidence: 99%

Annual Evaluation of 17 Oestrogenic Endocrine Disruptors and Hazard Indexes in the Douro River Estuary—The Atlantic Discharge of the Highest-Flow River of Southwestern Europe

Silva

2022

Water

Jurnal Akuakultur Indonesia

“…During the maturation period, the yolk deposition stage occurs in hepatocytes characterized by enlarged size of hepatocytes and increased basophilic cytoplasmic granules, and nucleoli undergo hypertrophy. Hepatocyte binucleate was found to increase and sinusoids with well vascularization (Chakrabarti & Chatterjee, 2014;Rajeshkumar, 2015, Abdallah et al, 2013Pinto et al, 2010;Tayel et al, 2014;Yilmaz et al, 2016). GSI and HSI in Mullet fish has fluctuative value based on spesies and location (Abdalhafid & El-Mor, 2014;El-Halfawy et al, 2007;El-Boray et al, 2012;Ghaninejad et al, 2010;Hashemi et al, 2012;Karim, 2017;Maryam et al, 2011;Nahdi et al, 2008;Sulistiono et al, 2001;Mokhtar et al, 2015;Saoudi & Aun., 2014;Wigati & Syafei., 2013;Yones et al, 2016).…”

Section: Gonadosomatic Index (Gsi) Value and Hepatosomatic Index (Hsimentioning

confidence: 99%

Stimulation of gonad maturation in mullet fish Mugil dussumieri using MT, E2, hCG, and Ovaprim hormone

Cahyono

Zairin

Carman

2019

The present study is a preliminary research for producing mullet fry to aquaculture. The research aimed to evaluate stimulation of gonad maturation in mullet (Mugil dussumieri) using hormones. The method used for research was completely randomized design consisting of three treatments and each individual replication was repeated three times. Two experiments were conducted separately with different treatments. First experiment used 9-14.7 cm body length of fish treated with different hormone injections i.e. 4 mg/kg 17α-methyltestosterone (MT), 0.07 mg/kg estradiol-17β (E2), and 0.5 ml/kg 0.9% physiological solution as control. Second experiment used 10-31 cm body length fish treated with 750 IU/kg chorionic gonadotropin (hCG), 0.5 ml/kg Ovaprim, and 0.9% physiological solution as control. Gonadosomatic index (GSI) value of the first experiment on day 60 showed that GSI of E2 treatment (1.31±0.94%) was higher than both MT treatment (1.00±0.51%) and control (0.54±0.20%). On the other hand, the second experiment on day 60 showed that GSI of hCG treatment (7.18±0.59%) was higher than both Ovaprim treatment (3.29±2.66%) and control (6.72±0.32%). Egg diameter frequency distribution for control in the first experiment on day 30 showed that egg size ranged from 9−144 µm. Egg diameter for E2 and MT treatments on day 60 showed that egg size ranged from 9−243 µm and were higher than control. In the second experiment, egg diameter on day 30 for control showed that egg size ranged from 9-144 µm, hCG treatment showed egg size ranged from 9−441 µm, while Ovaprim egg size ranged from 9-111 µm. Blood glucose, blood cholesterol, testosterone and estradiol hormone level in the first and second experiment showed no significant difference. The results showed that estradiol-17β and 17α-methyltestosterone induction in 9-14.7 cm body length mullet increase gonad maturity to stage II while hCG induction in 10-31 cm body length mullet increase gonad maturity to stage III. Keywords : estradiol-17β, hCG, 17α-methyltestosterone, Mugil dussumieri, Ovaprim ABSTRAK Penelitian merupakan rintisan untuk menghasilkan benih ikan belanak dalam wadah budidaya. Penelitian ini bertujuan untuk mengevaluasi pematangan gonad ikan belanak Mugil dussumieri menggunakan hormon. Penelitian dilakukan menggunakan rancangan acak lengkap yang terdiri dari tiga perlakuan dan tiga kali ulangan individu. Terdapat dua percobaan dengan perlakuan berbeda dan dilakukan secara terpisah. Percobaan pertama menggunakan ikan berukuran 9−14.7 cm dengan hormon 17α-metiltestosteron (MT) 4 mg/kg, estradiol-17β (E2) 0.07 mg/kg dan kontrol larutan fisiologis 0.9% 0.5 ml/kg. Percobaan kedua menggunakan ikan berukuran 10−31 cm dengan human chorionic gonadotropin (hCG) 750 IU/kg, Ovaprim 0.5 ml/kg dan kontrol larutan fisiologis 0.9 % 0.5 ml/kg. Nilai gonadosomatic index (GSI) percobaan pertama pada hari ke-60 menunjukkan bahwa pemberian estradiol-17β (1.31±0.94%) lebih tinggi dibandingkan 17α-metiltestosteron (1.00±0.51%) dan kontrol (0.54±0.20%). Hasil percobaan kedua pada hari ke-60 nilai GSI menunjukkan bahwa pemberian hCG (7.18±0.59%) lebih tinggi dibandingkan Ovaprim (3.29±2.66%) dan kontrol (6.72±0.32%). Sebaran frekuensi diameter telur pada percobaan pertama untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm. Sebaran frekuensi diameter telur untuk estradiol-17β dan 17α-metiltestosteron hari ke-60 menunjukkan kisaran 9−243 µm lebih banyak dibandingkan kontrol. Sebaran frekuensi diameter telur pada percobaan kedua untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm, hCG 9−441 µm sedangkan Ovaprim hanya 9−111 µm. Kadar glukosa darah, kolesterol darah, hormon testosteron dan estradiol pada percobaan pertama dan percobaan kedua menunjukkan tidak terdapat perbedaan yang nyata. Hasil penelitian menunjukkan bahwa induksi hormon estradiol-17β dan 17α-metiltestosteron pada ikan berukuran 9−14.7 cm dapat meningkatkan kematangan gonad mencapai TKG II sedangkan induksi hormon hCG pada ikan berukuran 10−31 cm dapat meningkatkan kematangan gonad mencapai TKG III. Kata kunci : estradiol-17β, hCG, 17α-metiltestosteron, Mugil dussumieri, Ovaprim

Water Environment Research

“…Xie et al (2010b) reported that both male and female Gambusia affinis in the Hanxi River of southern China showed significant morphological changes that were likely due to heavy contamination from municipal wastewater. Pinto et al (2010) used a combination of liver histology in two fish species (Mugil cephalus and Gobio gobio), as well as macroinvertebrate indices to describe the impact of a wastewater treatment plant on the Febros River in Portugal. Breckels and Neff (2010) described behavioral changes that were observed in brown bullheads (Ameiurus nebulosus) chronically exposed to pollution in the Detroit River (USA).…”

Section: Wastewatermentioning

confidence: 99%

Effects of Pollution on Freshwater Organisms

Harmon¹,

Wiley²

2011

This review includes works published in the general scientific literature during 2010 on the effects of anthropogenic pollutants on freshwater organisms. It begins with two broad sections: research reviews and broad field studies and surveys. This is followed by reviews of research categorized in sections to reflect the pollutant class. These sections include wastewater, stormwater and non‐point source pollution, nutrients, sediment cap materials and suspended clays, botanical extracts, surfactants, metals, persistent organic pollutants, pharmaceuticals, endocrine disruptors, pesticides, petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), ionic liquids, and nanomaterials. The final section includes works describing innovations in the field of freshwater pollution research.