Multi-block poloxamer surfactants suppress aggregation of denatured proteins

Mustafi, Devkumar; Smith, Catherine M.; Makinen, Marvin W.; Lee, Raphael C.

doi:10.1016/j.bbagen.2007.08.017

Cited by 42 publications

(32 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alexandridis, Holzwarth, and Hatton (1994) found that a negative entropy contribution caused by the removal of highly oriented clathrate cages of water molecules surrounding the non-polar block of Pluronics is the main driving force of micellarization. PF-68 was shown to facilitate re-folding and suppress the aggregation of thermally unfolded proteins (Mustafi, Smith, Makinen, & Lee, 2008). The model for surfactant-protein interaction (Lee et al, 2011) showed that in the case of a low-surfactant concentration below the CMC, the molecules of the surfactant are localized on the surface of the liquid phase and only a limited number of surfactant molecules are bound to the surface of the protein.…”

Section: Discussionmentioning

confidence: 99%

Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

Buzgo

Filová

Staffa

et al. 2017

J Tissue Eng Regen Med

View full text Add to dashboard Cite

In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

Buzgo

Filová

Staffa

et al. 2017

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…This non-ionic surfactant, comprised of hydrophobic and hydrophilic segments will surround and protect the protein by limiting its adsorption onto the hydrophobic surface of the PLGA polymer (Morille et al, 2013;Mustafi et al, 2008). It was co-precipitated with CST at the 415 ratio of 1:20 of protein:additive polymer, and allowed a complete release of CST.…”

Section: Discussionmentioning

confidence: 99%

Nanoprecipitated catestatin released from pharmacologically active microcarriers (PAMs) exerts pro-survival effects on MSC

Angotti

Venier-Julienne

Penna

et al. 2017

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Catestatin (CST), a fragment of Chromogranin-A, exerts angiogenic, arteriogenic, vasculogenic and cardioprotective effects. CST is a very promising agent for revascularization purposes, in "NO-OPTION" patients. However, peptides have a very short half-life after administration and must be conveniently protected. Fibronectin-coated 30 pharmacologically active microcarriers (FN-PAM), are biodegradable and biocompatible polymeric microspheres that can convey mesenchymal stem cell (MSCs) and therapeutic proteins delivered in a prolonged manner. In this study, we first evaluated whether a small peptide such as CST could be nanoprecipitated and incorporated within FN-PAMs.

show abstract

“…Besides the effects on MMPs, surfactants can also suppress protein aggregation and aid refolding of denatured proteins to prevent persistent inflammation, which may lead to a non‐healing wound. Mustafi et al found that amphiphilic, surfactant, multi‐block copolymers, such as Poloxamer 108 (P108), Poloxamer 188 (P188), and Tetronic 1107 (T1107) are efficient as additives to suppress aggregation of and to facilitate refolding of heat‐denatured hen egg white lysozyme (HEWL) and bovine serum albumin (BSA) in solution. Additionally, Lee et al demonstrated that poloxamers are more efficient than PEG in preventing aggregation of heat denaturized lysozyme.…”

Section: Mode Of Action Of Surfactants In Wound Healingmentioning

confidence: 99%

Mode of action of poloxamer‐based surfactants in wound care and efficacy on biofilms

Percival¹,

Chen²,

Mayer

et al. 2018

International Wound Journal

View full text Add to dashboard Cite

Surfactants are widely used as detergents, emulsifiers, wetting agents, foaming agents, and dispersants in both the food and oil industry. Their use in a clinical setting is also common, particularly in wound care. Complicated or chronic wounds show clinical signs of delayed healing, persistent inflammation, and the production of non-viable tissue. These types of wounds also present challenges such as infection and potentially house antimicrobial-tolerant biofilms. The use of wound cleansers to aid cleaning and debridement of the wound is essential. A large proportion of skin and wound cleansers contain surfactants but there is only a small amount of data that shows the effectiveness of them in the enhancement of wound closure. This review paper aims to explore the available literature surrounding the use and mode of action of surfactants in wound healing, in particular Poloxamer 188 (Pluronic F-68) and Poloxamer 407 (Pluronic F-127), and also uncover the potential mechanisms behind the enhancement of wound healing and comparison to other surfactants used in wound care. Furthermore, the presence of a microbial biofilm in the wound is a significant factor in delayed wound healing. Therefore, the effect of clinically used surfactants on biofilms will be discussed, with emphasis on poloxamer-based surfactants.

show abstract

Multi-block poloxamer surfactants suppress aggregation of denatured proteins

Cited by 42 publications

References 35 publications

Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

Needleless emulsion electrospinning for the regulated delivery of susceptible proteins

Nanoprecipitated catestatin released from pharmacologically active microcarriers (PAMs) exerts pro-survival effects on MSC

Mode of action of poloxamer‐based surfactants in wound care and efficacy on biofilms

Contact Info

Product

Resources

About