Multi‐Colored Fibers by Self‐Assembly of DNA, Histone Proteins, and Cationic Conjugated Polymers

Wang, Fengyan; Liu, Zhang; Wang, Bing; Feng, Liheng; Liu, Libing; Lv, Fengting; Wang, Yilin; Wang, Shu

doi:10.1002/ange.201308795

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…50 In this study, the CPEPS-OS-miR nanoparticles were formed by self-assembly of the oppositely charged CPEPS and plasmids mixture in aqueous solution. 51 To examine the surface charge of the self-assembled nanoparticles, zeta potential analysis was conducted. As a result, the CPEPS-OSmiR nanoparticles had positive charges, which not only can facilitate the encapsulation of the plasmids, but also may benefit the process of approaching cell membrane.…”

Section: ■ Discussionmentioning

confidence: 99%

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

Deng

Cao

Chen

et al. 2015

ACS Appl. Mater. Interfaces

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Induced pluripotent stem cells (iPSCs), resulting from the forced expression of cocktails out of transcription factors, such as Oct4, Sox2, Klf4, and c-Myc (OSKM), has shown tremendous potential in regenerative medicine. Although rapid progress has been made recently in the generation of iPSCs, the safety and efficiency remain key issues for further application. In this work, microRNA 302-367 was employed to substitute the oncogenic Klf4 and c-Myc in the OSKM combination as a safer strategy for successful iPSCs generation. The negatively charged plasmid mixture (encoding Oct4, Sox2, miR302-367) and the positively charged cationized Pleurotus eryngii polysaccharide (CPEPS) self-assembled into nanosized particles, named as CPEPS-OS-miR nanoparticles, which were applied to human umbilical cord mesenchymal stem cells for iPSCs generation after characterization of the physicochemical properties. The CPEPS-OS-miR nanoparticles possessed spherical shape, ultrasmall particle size, and positive surface charge. Importantly, the combination of plasmids Oct4, Sox2, and miR302-367 could not only minimize genetic modification but also show a more than 50 times higher reprogramming efficiency (0.044%) than any other single or possible double combinations of these factors (Oct4, Sox2, miR302-367). Altogether, the current study offers a simple, safe, and effective self-assembly approach for generating clinically applicable iPSCs.

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Section: ■ Discussionmentioning

confidence: 99%

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

Deng

Cao

Chen

et al. 2015

ACS Appl. Mater. Interfaces

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“…All organic solvents were purchased from Beijing Chemical Works and used as received. Compound 1 , 7 and 8 were synthesized according to procedures reported in the literatures35363738. PT was synthesized using previously reported procedures36 and a further anion exchange procedure.…”

Section: Methodsmentioning

confidence: 99%

“…Compound 1 , 7 and 8 were synthesized according to procedures reported in the literatures35363738. PT was synthesized using previously reported procedures36 and a further anion exchange procedure. A549 cell lines were purchased from Cell Culture Center of Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Water-Soluble Polythiophene Derivatives for Cell Imaging

Wang

et al. 2015

Sci Rep

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In this work, four water-soluble polythiophene derivatives (PT, PT-DDA, PT-ADA, and PT-ADA-PPR) with different pendant moieties were synthesized via oxidative copolymerization by FeCl3. By increasing the hydrophobic ability of side chain moieties, there is a gradually blue shift for the maximum absorption wavelength and red shift for the maximum emission wavelength, a reducing trend for fluorescence quantum yields, a growing trend for Stokes shift, and an increasing trend for the mean sizes in the order of PT, PT-ADA, and PT-DDA. All the synthesized polymers show low toxicity and good photostability and accumulate in the lysosomes of A549 cells. Furthermore, the introduction of porphyrin group to PT-ADA side chain (PT-ADA-PPR) broadens the absorption and emission ranges of PT-ADA. PT-ADA-PPR could be excited at two different excitation wavelengths (488 nm and 559 nm) and exhibits two emission pathways, and dual-color fluorescence images (orange and red) of PT-ADA-PPR accumulated in A549 cells are observed. Thus, PT-ADA-PPR could be used as an excellent dual-color fluorescent and lysosome-specific imaging material.

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“…Nonviral vectors are promising for effective gene delivery due to their good immunocompatibility and transfection performance. − Compared with liposome, polycations draw much more attention because of their simple modification and flexible design strategies. − A number of polycations, such as polyethylenimine (PEI), , polyamidoamine, poly(2-(dimethylamino) ethyl methacrylate), , and poly( l -lysine), exhibit some favorable properties in gene delivery applications. However, the high cytotoxicity caused by positive charges is still an obstacle to the application of polycations.…”

Section: Introductionmentioning

confidence: 99%

PGMA-Based Cationic Nanoparticles with Polyhydric Iodine Units for Advanced Gene Vectors

Sun

et al. 2016

Bioconjugate Chem.

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It is crucial for successful gene delivery to develop safe, effective, and multifunctional polycations. Iodine-based small molecules are widely used as contrast agents for CT imaging. Herein, a series of star-like poly(glycidyl methacrylate) (PGMA)-based cationic vectors (II-PGEA/II) with abundant flanking polyhydric iodine units are prepared for multifunctional gene delivery systems. The proposed II-PGEA/II star vector is composed of one iohexol intermediate (II) core and five ethanolamine (EA) and II-difunctionalized PGMA arms. The amphipathic II-PGEA/II vectors readily self-assemble into well-defined cationic nanoparticles, where massive hydroxyl groups can establish a hydration shell to stabilize the nanoparticles. The II introduction improves cell viabilities of polycations. Moreover, by controlling the suitable amount of introduced II units, the resultant II-PGEA/II nanoparticles can produce fairly good transfection performances in different cell lines. Particularly, the II-PGEA/II nanoparticles induce much better in vitro CT imaging abilities in tumor cells than iohexol (one commonly used commercial CT contrast agent). The present design of amphipathic PGMA-based nanoparticles with CT contrast agents would provide useful information for the development of new multifunctional gene delivery systems.

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Multi‐Colored Fibers by Self‐Assembly of DNA, Histone Proteins, and Cationic Conjugated Polymers

Cited by 13 publications

References 40 publications

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

Synthesis and Characterization of Water-Soluble Polythiophene Derivatives for Cell Imaging

PGMA-Based Cationic Nanoparticles with Polyhydric Iodine Units for Advanced Gene Vectors

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