Multi-component clobetasol-loaded monolithic lipid-polymer hybrid nanoparticles ameliorate imiquimod-induced psoriasis-like skin inflammation in Swiss albino mice

Pukale, Sudeep Sudesh; Sharma, Saurabh; Dalela, Manu; Singh, Arihant Kumar; Mohanty, Sujata; Mittal, Anupama

doi:10.1016/j.actbio.2020.08.020

Cited by 61 publications

(39 citation statements)

References 85 publications

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“…As of now, we could find only a few studies done using the Swiss model. 30,31 Therefore, in the present study, we developed a psoriasis model in Swiss albino mice and Balb/c mice and compared the disease stability and severity. Clobetasol is used as a standard drug to validate the models.…”

Section: Discussionmentioning

confidence: 99%

Differential Psoriatic Effect of Imiquimod on Balb/c and Swiss Mice

Salwa

Badanthadka

D’Souza³

2021

Journal of Health and Allied Sciences NU

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Introduction The influence of animal strain on psoriasis model development by imiquimod (IMQ) has been studied in Balb/c and Swiss mice. Materials and Methods Female mice of either strain were challenged with 5% IMQ (62.5 mg on back skin, 10 mg on right ear). They were observed for the severity of the disease using Psoriasis area severity index (PASI), splenomegaly, and histopathological alterations. To validate the model, well-established antipsoriatic drug clobetasol (0.05%, 120 mg on the back skin, 10 mg on the right ear) was used. Additionally, to study the strain-dependent response to IMQ associated with oxidative stress, various antioxidant factors like superoxide dismutase (SOD), catalase (CT), and glutathione (GSH) were measured. Antioxidant natural product curcumin (1%, 150 mg on back skin, 12.5 mg on right ear) was used to evaluate the alleviation of oxidative stress on distinct mice strain. Results PASI score, body weight, and histopathology indicated the development of disease in both the strains, severity, and stability of which was dependent on the particular strain. Splenomegaly suggested the systemic effect, which was comparable in both the stains. IMQ and its involvement in redox status were confirmed by an alteration in the activity of SOD, CT, and levels of GSH. Conclusion This study demonstrated that, in the IMQ-induced psoriasis model, the genetic background has some impact on the disease severity, stability, and redox imbalance.

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Section: Discussionmentioning

confidence: 99%

Differential Psoriatic Effect of Imiquimod on Balb/c and Swiss Mice

Salwa

Badanthadka

D’Souza³

2021

Journal of Health and Allied Sciences NU

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“…So far, these systems display improved performance in terms of loading capacity, controlled drug release, biocompatibility, and stability [111]. Thus, Pukale and coauthors [110] designed monolithic lipid-polymer hybrid nanoparticles to load clobetasol propionate for the topical management of psoriasis. The physicochemical properties of the hybrid nanoparticles were promising with average size of 95 nm, PDI of 0.213, and encapsulation efficiency of 84%.…”

Section: Hybrid Nanoparticlesmentioning

confidence: 99%

“…In vitro and ex vivo studies also showed that these nanocarriers display sustained drug release for 7 days, enhanced cellular uptake in HaCat cells, and high skin penetration to dermis and viable epidermis of psoriatic skin from Swiss albino mice. Furthermore, preclinical efficacy studies on Swiss albino mice with psoriasis-like inflammation showed that the treatment with a gel containing the hybrid nanocarriers improved the therapeutic efficacy compared to a marketed product [110].…”

Section: Hybrid Nanoparticlesmentioning

confidence: 99%

Nanodelivery Strategies for Skin Diseases with Barrier Impairment: Focusing on Ceramides and Glucocorticoids

et al. 2022

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The human epidermis has a characteristic lipidic composition in the stratum corneum, where ceramides play a crucial role in the skin barrier homeostasis and in water-holding capacity. Several skin diseases, such as atopic dermatitis and psoriasis, exhibit a dysfunction in the lipid barrier with altered ceramide levels and increased loss of transepidermal water. Glucocorticoids are normally employed in the therapeutical management of these pathologies. However, they have shown a poor safety profile and reduced treatment efficiency. The main objective of this review is to, within the framework of the limitations of the currently available therapeutical approaches, establish the relevance of nanocarriers as a safe and efficient delivery strategy for glucocorticoids and ceramides in the topical treatment of skin disorders with barrier impairment.

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“…In vivo antipsoriatic activity [33] Lecithin/chitosan nanoparticles Ionic interaction Evaluation of skin barrier function and damage [34] Chitosan patches Electrophoretic deposition For fast drug delivery in oral mucosa disease [35] Squarticles (nanoemulgels) Homogenization method Enhancing the better permeation, increasing skin retention [36] PLGA microspheres Oil/water emulsion-solvent evaporation method…”

Section: Nanoprecipitation-solvent Evaporation Techniquementioning

confidence: 99%

Novel Dermal Delivery Cargos of Clobetasol Propionate: An Update

et al. 2022

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Dermal disorders such as psoriasis and eczema are associated with modifications in the chemical and molecular composition of the skin. Clobetasol propionate (CP), a superpotent topical glucocorticoid, is widely used for the therapeutic management of various skin conditions, owing to its strong anti-inflammatory, antipruritic, vasoconstrictive, and antiproliferative activities. Safety studies demonstrated that CP is safer for a shorter period, however, with prolonged application, it shows secondary side effects such as photosensitivity, Cushing-like syndrome, allergic contact dermatitis, osteonecrosis, hypopigmentation, steroid acne, and skin atrophy. Therefore, the US FDA (United States Food and Drug Administration) has restricted the usage of CP to not more than 15 days. Research scientists addressed its several formulations and drug delivery issues, such as low water solubility, less stability, photodegradation, and poor absorption, by incorporating them into novel nanobased delivery platforms. With the utilization of these technologies, these drawbacks of CP have been resolved to a large extent to reestablish this moiety. This article explores the physicochemical properties and mechanism of action of CP. Additionally, an attempt has been made to discover and highlight the possible features of the novel nanosystems, including nanoemulsions, nanosponges, solid lipid nanoparticles, nanostructured lipid carriers, and nanogels, reported for CP. The stability and safety concerns of CP, along with its commercial status, are also discussed.

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Multi-component clobetasol-loaded monolithic lipid-polymer hybrid nanoparticles ameliorate imiquimod-induced psoriasis-like skin inflammation in Swiss albino mice

Cited by 61 publications

References 85 publications

Differential Psoriatic Effect of Imiquimod on Balb/c and Swiss Mice

Differential Psoriatic Effect of Imiquimod on Balb/c and Swiss Mice

Nanodelivery Strategies for Skin Diseases with Barrier Impairment: Focusing on Ceramides and Glucocorticoids

Novel Dermal Delivery Cargos of Clobetasol Propionate: An Update

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