Multi-component HPLC Fingerprinting of Radix Salviae Miltiorrhizae and Its LC-MS-MS Identification

Hu, Ping; Liang, Qiong; Luo, Gang; Zhao, Zhongzhen; Jiang, Zhi Hong

doi:10.1248/cpb.53.677

Cited by 137 publications

(104 citation statements)

References 12 publications

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“…Pharmacol-ogical studies established that these compounds possessed anti-ischemic, antioxidant, and antitumor activities [9,10]. Some analytical techniques were utilized for separation and identification of constituents in RSM, such as high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC), and liquid chromatography hyphenated with tandem mass spectrometry (LC-MS/MS) [11][12][13]. However, HPTLC had a disadvantage of low resolution, which resulted in inaccuracy.…”

Section: Introductionmentioning

confidence: 99%

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

Yang¹,

Wu²,

Rui³

et al. 2015

Acta Chromatographica

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Summary.A rapid method has been used for simultaneous identification of both hydrophilic and lipophilic compounds from Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza B GE .) by ultra-performance liquid chromatography/quadrupole time-offlight mass spectrometry (UPLC/Q-TOF-MS). A total of 58 compounds extracted by methanol were detected and tentatively identified within 20 min, including hydrophilic phenolics, lipophilic diterpenoids, a verbascose, and several organic acids. These compounds were separated on an Acquity UPLC BEH C18 column and identified based on tandem mass spectrometry (MS/MS) fragmentation patterns under the positive and negative ion modes, respectively. Among them, micranthin B and 9-oxo-10E,12Z-octadecadienoic acid were reported in RSM for the first time. Their fragmentation patterns in electrospray ionization (ESI)-MS/MS spectra were first investigated by matching their accurate molecular masses. This contribution presented one of the first reports on the analysis of hydrophilic phenolics and lipophilic diterpenoids from Radix Salviae Miltiorrhizae using UPLC/Q-TOF-MS. The results demonstrated that UPLC/Q-TOF-MS method could be applied to rapidly and expediently describe and provide comprehensive chemical information for simultaneous analysis of two different polar components in RSM.

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Section: Introductionmentioning

confidence: 99%

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

Yang¹,

Wu²,

Rui³

et al. 2015

Acta Chromatographica

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“…The current leading application of Salvia miltiorrhiza Bunge is in cardiovascular medicine. Phytochemical studies revealed multiple groups of compounds from Salvia miltiorrhiza Bunge extract, the main constituents of which include tanshinones (eg, diterpene chinolones, lipophilic) and phenolics (eg, protocatechuic aldehyde or salvianolic acid B, hydrophilic) [9][10] . SAA is contained in lower quantities in the aqueous extract of Salvia miltiorrhiza, but can be derived and bio-transformed in vivo from salvianic acid B. SAA is the simplest structure and the most effective component of Salvia miltiorrhiza.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic effects of D(+)β-3,4-dihydroxyphenyl lactic acid (salvianic acid A, SAA) on osteoblasts and bone marrow stromal cells of intact and prednisone-treated rats

Cui

Liu

et al. 2009

Acta Pharmacol Sin

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Aim:Previous studies have shown that D(+)β-3,4-dihydroxyphenyl lactic acid (salvianic acid A, SAA) has anabolic effects on prednisone (GC)-induced osteoporosis in rats. The current study aims to investigate the molecular mechanism of SAA's impact on osteogenesis and adipogenesis in bone marrow stromal cells in intact and GC-treated rats. Methods: For in vitro study, newborn rat calvaria osteoblasts (rOBs) and rat bone marrow stromal cells (rMSCs) were isolated, identified and cultured with SAA at different concentrations to evaluate SAA's influence on osteogenesis and adipogenesis. In addition, 3-month-old Sprague-Dawley (SD) male rats were treated with distilled water, prednisone alone (3.0 mg·kg -1 ·d -1 ) or prednisone (3.0 mg·kg -1 ·d -1 ) and SAA (25 mg·kg -1 ·d -1 ) for 45 d. At the end point, the different groups of rMSCs were isolated by density-gradient centrifugation and cultured. Results: (1) At 0.1-10.0 mg/L, SAA increased ALP activity, type I collagen (Coll-I) mRNA and OPG mRNA expression and stimulated nodule mineralization of rOBs. SAA (0.5 mg/L) also significantly increased the ALP activity of rMSCs without a need for osteogenesis-inducing medium. At 5.0 mg/L, SAA decreased the number of adipocytes with less lipid droplet formation from the rMSCs, which typically undergo adipocyte induction. (2) Coll-I expression was markedly decreased, whereas lipoprotein lipase (LPL) mRNA expression increased by 98% when compared with the first generation of rMSCs in GC-treated rats. The SAA-treated rats demonstrated an over 2-fold increase in Coll-I expression when compared with intact rats and further showed a significant decrease in LPL expression when compared with GC-treated rats. When rMSCs were co-cultured with SAA (0.5 mg/L) in vitro, SAA did not affect Coll-I and LPL gene expression in intact rats but significantly increased Coll-I and decreased LPL gene expression in GC-treated rats. Conclusion: SAA protected bone from GC-induced bone marrow impairment by stimulating osteogenesis and depressing adipogenesis in bone marrow stromal cells both in vivo and in vitro. The data indicated that aqueous extract of Salvia miltiorrhiza, which include SAA, may serve as an active anabolic agent and a useful therapeutic strategy for the treatment of GC-associated osteoporosis.

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“…SalB is one of the major water-soluble compounds of Salvia miltiorrhiza (Danshen), which was one of the most popular Chinese herbs listed in the Chinese Pharmacopoeia and has been used for the treatment of ischemic cardiovascular and cerebrovascular diseases clinically in China for centuries [23,24]. As the most bioactive component of salvianolic acid extracted from Danshen, SalB shows higher free radical scavenging activity than vitamin C, and its antioxidant capacity is in agreement with its protective effects against cell injury from oxidative stresses [25].…”

Section: Discussionmentioning

confidence: 99%

Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties

Liu

Zheng

et al. 2015

Biochemical and Biophysical Research Communications

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Multi-component HPLC Fingerprinting of Radix Salviae Miltiorrhizae and Its LC-MS-MS Identification

Cited by 137 publications

References 12 publications

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

Osteogenic effects of D(+)β-3,4-dihydroxyphenyl lactic acid (salvianic acid A, SAA) on osteoblasts and bone marrow stromal cells of intact and prednisone-treated rats

Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties

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