Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties

Liu, Miao; Zheng, Mingjing; Xu, Hanying; Liu, Lianqing; Li, Yanchun; Xiao, Wei; Li, Jianchun; Ma, Enlong

doi:10.1016/j.bbrc.2015.10.127

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…Moreover, SalB inhibits tumor necrosis factor-alpha (TNF-alpha)-induced MMP-2 upregulation in human aortic smooth muscle cells via suppression of NAD(P)H oxidase-derived reactive oxygen species [29] . SalB also reduces the expressions of inflammatory factors, such as interleukins (IL-1β, IL-6, and IL-8), alleviates apoptosis of vascular cells to improve heart function, reduces liver and renal fibrosis via down-regulating transforming growth factor β1 (TGF-β1) and depressing MMP-2 activity [30] . SalB suppresses expression of COX-2, consequently retarding growth of cancer cells as demonstrated in both in vivo and in vitro studies [31] .…”

Section: Discussionmentioning

confidence: 99%

Salvianolic Acid B Alleviates Heart Failure by Inactivating ERK1/2/GATA4 Signaling Pathway after Pressure Overload in Mice

Chen

Tan

et al. 2016

PLoS ONE

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BackgroundHeart failure(HF) is a dangerous disease that affects millions of patients. Radix Salvia is widely used in Chinese clinics to treat heart diseases. Salvianolic acid B(SalB) is the major active component of Radix Salvia. This study investigated the mechanisms of action and effects of SalB on HF in an experimental mouse model of HF.MethodsWe created a mouse model of HF by inducing pressure overload with transverse aortic constriction(TAC) surgery for 2 weeks and compared among 4 study groups: SHAM group (n = 10), TAC group (n = 9), TAC+MET group (metprolol, positive drug treatment, n = 9) and TAC+SalB group (SalB, 240 mg•kg-1•day-1, n = 9). Echocardiography was used to evaluate the dynamic changes in cardiac structure and function in vivo. Plasma brain natriuretic peptide (BNP) concentration was detected by Elisa method. In addition, H9C2 rat cardiomyocytes were cultured and Western blot were implemented to evaluate the phosphorylation of ERK1/2, AKT, and protein expression of GATA4.ResultsSalB significantly inhibited the phosphorylation of Thr202/Tyr204 sites of ERK1/2, but not Ser473 site of AKT, subsequently inhibited protein expression of GATA4 and plasma BNP(P < 0.001), and then inhibited HF at 2 weeks after TAC surgery.ConclusionsOur data provide a mechanism of inactivating the ERK1/2/GATA4 signaling pathway for SalB inhibition of the TAC-induced HF.

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Section: Discussionmentioning

confidence: 99%

Salvianolic Acid B Alleviates Heart Failure by Inactivating ERK1/2/GATA4 Signaling Pathway after Pressure Overload in Mice

Chen

Tan

et al. 2016

PLoS ONE

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“…Salvianolic acid B (SalB) is one of the most bioactive component extracted from Salvia miltiorrhiza, which shows higher free radical scavenging activity than vitamin C, and its antioxidant capacity corresponds with its protective effects against cell injury from oxidative stresses [5]. In our previous study, SalB was screened to have a noticeably preventive effect on bleomycin (BLM)-induced pulmonary fibrosis in Wistar rats by characterizing the biophysical properties of TGF-b1-induced A549 cells [6]. In this study, we focused on the effect of SalB on myofibroblast transdifferentiation in TGF-b1-induced MRC-5 cells, the ability of SalB to protect against oxidative stress, and the possible mechanism of this protection.…”

Section: Introductionmentioning

confidence: 99%

Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2

Liu

Zhang

et al. 2018

Biochemical and Biophysical Research Communications

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“…The latest studies in different fields have suggested the anti-fibrotic activity of Sal B in multiple organ systems such as pulmonary fibrosis, hepatic fibrosis and oral submucous fibrosis [14–17, 26–28]. These studies revealed the ability of Sal B to prevent fibrotic changes both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Salvianolic acid B reduced the formation of epidural fibrosis in an experimental rat model

et al. 2016

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BackgroundSalvianolic acid B (Sal B) was newly reported to be able to attenuate fibrosis in the animal model. The aim of the present study was to investigate the effect of the intragastric application of Sal B on the prevention of epidural fibrosis (EF).MethodsForty healthy adult male Wistar rats were divided into four treatment groups (n = 10 per group): (1) 10 mg/kg Sal B, (2) 30 mg/kg Sal B, (3) 50 mg/kg Sal B and (4) Saline (vehicle treatment, control group). All animals underwent a laminectomy at the lumbar 1–2 (L 1–2) level. After intragastric treatment, all rats were sacrificed at post-operative week 8. The extent of the epidural scar, the regeneration of the vasculature and the expression levels of vascular endothelial growth factor (VEGF) were analysed.ResultsThe animals’ recovery was uneventful during the experimental period. The extent of the epidural scar, the regeneration of the vasculature and the expression levels of VEGF suggested better outcomes in the Sal B-treated groups. Sal B exerted the ability to prevent the formation of an epidural scar and vascularization at the laminectomy sites. The effects of Sal B were dose-dependent, with the 50 mg/kg Sal B group showing the best outcomes compared with the other groups.ConclusionsPost-operative intragastric application of Sal B can prevent the formation of epidural scarring. Sal B exerted these effects in a dose-dependent manner, and 50 mg/kg dose was shown to be the best effect in the present study. The results of this study reveal that Sal B could be a potential therapy for EF and valuable for further research.

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Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties

Cited by 15 publications

References 26 publications

Salvianolic Acid B Alleviates Heart Failure by Inactivating ERK1/2/GATA4 Signaling Pathway after Pressure Overload in Mice

Salvianolic Acid B Alleviates Heart Failure by Inactivating ERK1/2/GATA4 Signaling Pathway after Pressure Overload in Mice

Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2

Salvianolic acid B reduced the formation of epidural fibrosis in an experimental rat model

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