Multi-drug resistance, integron and transposon-mediated gene transfer in heterotrophic bacteria from Penaeus vannamei and its culture environment

Nadella, R.K.; Panda, Satyen Kumar; Badireddy, Madhusudana Rao; Prasad, K. Pani; Raman, Rajagopalan; Mothadaka, Mukteswar Prasad

doi:10.1007/s11356-021-18163-1

Cited by 17 publications

(10 citation statements)

References 63 publications

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“…Bacterial MDR is regarded as an emerging pollutant in different food production avenues including aquaculture [ 94 ]. Resistance factors have been reported in pathogenic bacteria [ 68 , 69 , 70 , 71 , 72 , 95 , 96 , 97 , 98 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic and Transcriptomic Analysis Reveal Multiple Strategies for the Cadmium Tolerance in Vibrio parahaemolyticus N10-18 Isolated from Aquatic Animal Ostrea gigas Thunberg

Pan

Yang

Wang

et al. 2022

Foods

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The waterborne Vibrio parahaemolyticus can cause acute gastroenteritis, wound infection, and septicemia in humans. Pollution of heavy metals in aquatic environments is proposed to link high incidence of the multidrug-resistant (MDR) pathogen. Nevertheless, the genome evolution and heavy metal tolerance mechanism of V. parahaemolyticus in aquatic animals remain to be largely unveiled. Here, we overcome the limitation by characterizing an MDR V. parahaemolyticus N10-18 isolate with high cadmium (Cd) tolerance using genomic and transcriptomic techniques. The draft genome sequence (4,910,080 bp) of V. parahaemolyticus N10-18 recovered from Ostrea gigas Thunberg was determined, and 722 of 4653 predicted genes had unknown function. Comparative genomic analysis revealed mobile genetic elements (n = 11) and heavy metal and antibiotic-resistance genes (n = 38 and 7). The bacterium significantly changed cell membrane structure to resist the Cd2+ (50 μg/mL) stress (p < 0.05). Comparative transcriptomic analysis revealed seven significantly altered metabolic pathways elicited by the stress. The zinc/Cd/mercury/lead transportation and efflux and the zinc ATP-binding cassette (ABC) transportation were greatly enhanced; metal and iron ABC transportation and thiamine metabolism were also up-regulated; conversely, propanoate metabolism and ribose and maltose ABC transportation were inhibited (p < 0.05). The results of this study demonstrate multiple strategies for the Cd tolerance in V. parahaemolyticus.

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Section: Discussionmentioning

confidence: 99%

Genomic and Transcriptomic Analysis Reveal Multiple Strategies for the Cadmium Tolerance in Vibrio parahaemolyticus N10-18 Isolated from Aquatic Animal Ostrea gigas Thunberg

Pan

Yang

Wang

et al. 2022

Foods

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“…Other resistant determinants such as quinolones ( qnrA ), AmpC‐β‐lactamases, chloramphenicol‐resistant genes ( catA1 , catA2 , catA3 , and catB3 ) were not found in E. coli isolates with the corresponding phenotypic resistance. The AMR in non‐pathogenic E. coli reported in this study is significant since the transfer of resistance genes can take place between closely related bacteria such as with other members of the Enterobacteriaceae family (Grabow, Middendorff, & Prozesky, 1973; Guerra et al, 2003; Nadella et al, 2022). Lack of perfect correlation between the phenotypic resistance and its corresponding genetic determinants indicates that E. coli could have adopted other mechanisms of resistance such as co‐resistance and cross‐resistance.…”

Section: Resultsmentioning

confidence: 88%

“…Genes encoding resistance for sulfonamides (sul1) and trimethoprim (dfrA) were distributed in 9% of the isolates. (Grabow, Middendorff, & Prozesky, 1973;Guerra et al, 2003;Nadella et al, 2022).…”

Section: Screening Of Args In Drug-resistant E Coli Isolatesmentioning

confidence: 99%

Multidrug resistance of Escherichia coli in fish supply chain: A preliminary investigation

Kusunur

Kuraganti

Mogilipuri

et al. 2022

Journal of Food Safety

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Ninety‐five Escherichia coli isolates recovered from different supply points of freshwater fish namely fish pond (W1, n = 16), wholesale fish market (W2, n = 14), retail fish market (R1, n = 29), street fish vending unit (R2, n = 30), and modern fish vending unit (R3, n = 6) were investigated for antimicrobial‐resistant profile and prevalence of antibiotic‐resistant genes. Overall, multidrug‐resistant (MDR) E. coli in the fish supply chain was 21% with higher MDR incidence in the downstream fish supply points. MDR was relatively higher in the tertiary fish supply points viz., retail fish market (45%), street fish vending unit (30%), and modern fish vending unit (15%) compared to the secondary fish supply point, that is, wholesale fish market (5%) and primary fish supply point, that is, fish pond (5%). All the ampicillin‐resistant isolates carried ampC gene, whereas only 21% of the β‐lactam resistant isolates carried blaCTX‐M‐gp1. The tetracycline resistance determinant, tetA (89%) was predominant in the tetracycline‐resistant isolates compared to tetB and tetD (11%). The trimethoprim resistance gene, dfrA and sulfonamide‐resistance gene, Sul1 were detected in 9% of the trimethoprim‐resistant isolates and 9% of the sulfonamide‐resistant isolates, respectively. Enterobacterial repetitive intergeneric consensus‐PCR has delineated the MDR isolates from the different fish supply points into three major clusters but all the MDR isolates from the street fish vending point are grouped into a single cluster. The results indicate an increase in the proportion of MDR E. coli and the occurrence of diverse MDR profiles in the downstream points of the fish supply chain that needs to be addressed to avoid fish‐food‐borne antimicrobial resistance.

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“…However, the proteins described to confer resistance to oxytetracycline (i.e., TetQ [ 92 ] and TetR [ 93 , 94 ]) were identified. Other genomic investigations have described similar tetQ or tetR genes in T. dicentrarchi [ 59 ], T. piscium [ 12 ], and T. aestuarii [ 95 ]. Other proteins annotated in all ten barcodes may help confer a basal tolerance or resistance to antimicrobials, including, but not limited to: efflux pumps (multidrug resistance protein NorM, multidrug ABC transporter, CusA/CzcA family heavy metal efflux RND transporter); peroxide stress resistance (peroxide stress resistance protein YaaA, superoxide dismutase); and transcriptional regulators (multiple antibiotic resistance protein MarR).…”

Section: Discussionmentioning

confidence: 99%

Genomics of Tenacibaculum Species in British Columbia, Canada

et al. 2023

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Tenacibaculum is a genus of Gram-negative filamentous bacteria with a cosmopolitan distribution. The research describing Tenacibaculum genomes stems primarily from Norway and Chile due to their impacts on salmon aquaculture. Canadian salmon aquaculture also experiences mortality events related to the presence of Tenacibaculum spp., yet no Canadian Tenacibaculum genomes are publicly available. Ribosomal DNA sequencing of 16S and four species-specific 16S quantitative-PCR assays were used to select isolates cultured from Atlantic salmon with mouthrot in British Columbia (BC), Canada. Ten isolates representing four known and two unknown species of Tenacibaculum were selected for shotgun whole genome sequencing using the Oxford Nanopore’s MinION platform. The genome assemblies achieved closed circular chromosomes for seven isolates and long contigs for the remaining three isolates. Average nucleotide identity analysis identified T. ovolyticum, T. maritimum, T. dicentrarchi, two genomovars of T. finnmarkense, and two proposed novel species T. pacificus sp. nov. type strain 18-2881-AT and T. retecalamus sp. nov. type strain 18-3228-7BT. Annotation in most of the isolates predicted putative virulence and antimicrobial resistance genes, most-notably toxins (i.e., hemolysins), type-IX secretion systems, and oxytetracycline resistance. Comparative analysis with the T. maritimum type-strain predicted additional toxins and numerous C-terminal secretion proteins, including an M12B family metalloprotease in the T. maritimum isolates from BC. The genomic prediction of virulence-associated genes provides important targets for studies of mouthrot disease, and the annotation of the antimicrobial resistance genes provides targets for surveillance and diagnosis in veterinary medicine.

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Multi-drug resistance, integron and transposon-mediated gene transfer in heterotrophic bacteria from Penaeus vannamei and its culture environment

Cited by 17 publications

References 63 publications

Genomic and Transcriptomic Analysis Reveal Multiple Strategies for the Cadmium Tolerance in Vibrio parahaemolyticus N10-18 Isolated from Aquatic Animal Ostrea gigas Thunberg

Genomic and Transcriptomic Analysis Reveal Multiple Strategies for the Cadmium Tolerance in Vibrio parahaemolyticus N10-18 Isolated from Aquatic Animal Ostrea gigas Thunberg

Multidrug resistance of Escherichia coli in fish supply chain: A preliminary investigation

Genomics of Tenacibaculum Species in British Columbia, Canada

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