Multidrug resistance of <i>Escherichia coli</i> in fish supply chain: A preliminary investigation

Kusunur, Ahamed Basha; Kuraganti, George Kamal; Mogilipuri, Shanmukha Sai; Vaiyapuri, Murugadas; Narayanan, Sreejith Viyyokkaran; Badireddy, Madhusudana Rao

doi:10.1111/jfs.12972

Cited by 4 publications

(2 citation statements)

References 61 publications

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“…Additionally, the study identified the presence of sulfonamide resistance genes, sul 1 and sul 2. Another study, from India, also identified that 21% of fish Escherichia coli carried multidrug-resistant (MDR) genes, including the sulfonamide resistance gene sul 1 [38]. Therefore, the residues of sulfonamide antibiotics in aquatic products deserves more attention, and more extensive testing should be conducted to explore their residues and provide data to support the evaluation of the health risks from their consumption.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Health Risk Assessment of Sulfonamide Antibiotics in Different Freshwater Fish in Northeast China

Shen,

Yu,

et al. 2023

Toxics

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This study aimed to investigate the levels of 12 sulfonamide antibiotics in freshwater fish species obtained from three cities in northeastern China (Harbin, Changchun, and Shenyang). The analysis was conducted using HPLC–MS/MS to accurately quantify the antibiotic concentrations in the fish samples. The results showed that the average levels of sulfonamide antibiotics in fish samples from Harbin, Changchun, and Shenyang were 1.83 ng/g ww, 0.98 ng/g ww, and 1.60 ng/g ww, respectively. Sulfamethoxazole displayed the highest levels and detection rates in all three cities, whereas sulphapyridine exhibited the lowest concentrations in all the fish samples. The levels of sulfonamide antibiotic residues in the different fish species varied widely among the cities, and the highest level of antibiotic residues was found in the muscle of carnivorous fish. The results from a health risk evaluation on the consumption of these fish indicated that the risk from long-term antibiotic exposure to local residents from the intake of the sampled fish was small and not sufficient to pose a significant health risk to consumers.

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Section: Discussionmentioning

confidence: 99%

Occurrence and Health Risk Assessment of Sulfonamide Antibiotics in Different Freshwater Fish in Northeast China

Shen,

Yu,

et al. 2023

Toxics

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“…It is commonly isolated from fish intestines and found in contaminated water. E. coli has been seen invading other fish tissues such the gills, kidneys, muscles, and bladder (Kusunur et al 2022;Yohans et al 2022).…”

Section: E Colimentioning

confidence: 99%

Incidence, Transmission Mechanisms and Pathologic Implications of Bacterial Zoonotic Diseases of Fish

2023

IJAB

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Fish harbor a variety of bacterial pathogens, some of which can cause disease in humans. Zoonotic bacteria that may be present in asymptomatic fish hosts pose a public health risk when transmitted to humans through handling or consuming infected fish. Mycobacteriosis, caused by species in the Mycobacterium marinum group, is perhaps the most notorious zoonotic infection associated with fish and can produce serious skin infections in humans. Streptococcosis and staphylococcosis also occur with some frequency in fish and can result in human cases of septicemia, endocarditis or pneumonia if injured skin comes into contact with infected fish tissues and bacteria access wounds. Additionally, some Vibrio and Clostridium species found among fish may cause wound infections or gastrointestinal illness in humans, usually subsequent to exposure through handling or ingesting raw seafood. Clinical signs of bacterial zoonosis are variable and diagnosis in human cases can prove complicated by the vast diversity of potential pathogens involved. Preventative measures center on educating aquarists and fish handlers to avoid direct contact with ulcerated areas, lesions or feces from diseased fish. For consumer safety, good aquaculture practices that reduce bacterial loads in farmed fish stock are recommended. Moreover, thoroughly cooking fish to an internal temperature over 140°F destroys pathogens that may be present. Additional research priorities include better characterization of bacterial diversity among wild and farmed fish, investigating genetic and immunological aspects of disease resistance, developing improved diagnostics through genomic analysis, assessing efficacy of existing antibacterial treatments in clearing pathogens prior to human consumption and formulating integrated control strategies to mitigate risks. This book chapter examines the incidence, transmission mechanisms and pathologic implications of common bacterial zoonosis originating from fish hosts.

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Coliphage cocktails for controlling antimicrobial-resistantEscherichia coli: emphasizing polyphage and multihost interactions at different levels of multiplicity of infection

Benala

Vaiyapuri

Karthika

et al. 2023

Journal of Applied Microbiology

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Aim This study elucidates the in vitro bactericidal effectiveness of poly-phage cocktail combinations of 2, 4, 6, 8 and 10 individual coliphages against a cocktail of 20 AMR E. coli. Methods & results Different polyphage cocktails viz., 45 two-phage combinations, 28 four-phage combinations, 15 six-phage combinations, 6 eight-phage combinations and one ten-phage combination were formulated using a pool of ten coliphages that were isolated from two different geographical locations (East and West coasts of India). The different poly phage cocktails were tested at four different levels of Multiplicity of Infection (MOI) viz., MOI-1, MOI-10, MOI-100 and MOI-1000. All the 2, 4, 6, 8 and 10 phage cocktails were found to be effective in controlling the growth of a cocktail of 20 AMR bacteria when tested at MOI-1000 and MOI-100 but variations in antibacterial activity were observed at lower MOIs of 10 and 1. The ten coliphage cocktail showed lytic activity against 100% of AMR E. coli from farmed brackish water shrimp, 96% of laboratory collection of AMR E. coli, 92% of AMR E. coli from farmed freshwater fish and 85% of AMR E. coli from market shrimp. Conclusion Polyphage cocktails of 2, 4, 6, 8 and 10 coliphages applied at an MOI of 1000 effectively suppressed the growth of antimicrobial resistant E. coli. The results indicated phage-phage synergy in the lytic activity of several coliphage combinations at higher MOIs of 1000 and 100 while phage-phage antagonism was evidenced at lower MOIs of 10 and 1.

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Multidrug resistance of Escherichia coli in fish supply chain: A preliminary investigation

Cited by 4 publications

References 61 publications

Occurrence and Health Risk Assessment of Sulfonamide Antibiotics in Different Freshwater Fish in Northeast China

Occurrence and Health Risk Assessment of Sulfonamide Antibiotics in Different Freshwater Fish in Northeast China

Incidence, Transmission Mechanisms and Pathologic Implications of Bacterial Zoonotic Diseases of Fish

Coliphage cocktails for controlling antimicrobial-resistantEscherichia coli: emphasizing polyphage and multihost interactions at different levels of multiplicity of infection

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