Multi-effect of the water-solubleMoringa oleiferalectin againstSerratia marcescensandBacillussp.: antibacterial, antibiofilm and anti-adhesive properties

Moura, Márcio das Chagas; Trentin, Danielle da Silva; Napoleão, Thiago Henrique; Primon-Barros, Muriel; Xavier, André S.; Carneiro, N. P.; Paiva, Patrícia Maria Guedes; Macêdo, Alexandre José; Coelho, Luana Cassandra Breitenbach Barroso

doi:10.1111/jam.13556

Cited by 48 publications

(25 citation statements)

References 48 publications

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“…Some lectins also interfere with bacterial cell wall permeability. For example, one study conducted by Moura et al demonstrated that use of water soluble lectin from seeds of M. oleifera against corrosive bacteria caused leakage of intracellular proteins due to the loss of wall/membrane integrity in a dose dependent manner (Moura et al, 2017). Apart from the bactericidal and bacteriostatic effect, plant lectins also inhibit bacterial biofilm formation.…”

Section: Antibacterial Activity Of Plant Lectinsmentioning

confidence: 99%

Structure-function and application of plant lectins in disease biology and immunity

Mishra

Behura

Mawatwal

et al. 2019

Food and Chemical Toxicology

157

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A B S T R A C TLectins are proteins with a high degree of stereospecificity to recognize various sugar structures and form reversible linkages upon interaction with glyco-conjugate complexes. These are abundantly found in plants, animals and many other species and are known to agglutinate various blood groups of erythrocytes. Further, due to the unique carbohydrate recognition property, lectins have been extensively used in many biological functions that make use of protein-carbohydrate recognition like detection, isolation and characterization of glycoconjugates, histochemistry of cells and tissues, tumor cell recognition and many more. In this review, we have summarized the immunomodulatory effects of plant lectins and their effects against diseases, including antimicrobial action. We found that many plant lectins mediate its microbicidal activity by triggering host immune responses that result in the release of several cytokines followed by activation of effector mechanism. Moreover, certain lectins also enhance the phagocytic activity of macrophages during microbial infections. Lectins along with heat killed microbes can act as vaccine to provide long term protection from deadly microbes. Hence, lectin based therapy can be used as a better substitute to fight microbial diseases efficiently in future.

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Section: Antibacterial Activity Of Plant Lectinsmentioning

confidence: 99%

Structure-function and application of plant lectins in disease biology and immunity

Mishra

Behura

Mawatwal

et al. 2019

Food and Chemical Toxicology

157

View full text Add to dashboard Cite

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“…Although there is no clear mechanism, there is evidence that the plant's antimicrobial activity is related to a wide spectrum of bactericidal substances produced by the plant (Kostova and Dinchev, 2005;Viera et al, 2010). However, the seeds of M. oleifera also contain a water-soluble lectin called (WSMoL), which was previously reported to have antibacterial effects (Moura et al, 2015(Moura et al, , 2017Coriolano et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of polypyrrole nanoparticles and aqueous extract ofMoringa oleiferaagainstStaphylococcusspp. carriers of multi-drug efflux system genes isolated from dairy farms

Silva

Barros

Santos

et al. 2020

Journal of Dairy Research

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Our objectives were to identify genes of the multi-drug efflux system and to evaluate the antimicrobial activities of polypyrrole nanoparticles (PPy-NPs) and aqueous extract of Moringa oleifera against Staphylococcus spp. isolated from dairy farms in Northeast Brazil. Initially, 162 Staphylococcus spp. isolates were subjected to in vitro antimicrobial sensitivity tests. Of these, 35 presented antimicrobial multi-drug resistance phenotypes. These 35 isolates were then referred for the detection of norA, norB, norC, msrA, mgrA, tet-38, and lmrS genes, all of which feature in multi-drug efflux systems. In the isolates carrying the genes, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PPy-NPs and Moringa oleifera aqueous extract were determined. In the molecular analysis of the 35 isolates norA, norC, tet-38, and msrA genes were detected and for the other genes norB, lmrS and mgrA there was no amplification. Antimicrobial activity was verified of PPy-NPs and aqueous extract of Moringa oleifera in Staphylococcus spp. carrying multi-drug efflux system genes. We concluded that there are multi-drug efflux system genes present in the Staphylococcus spp. from the agricultural environment in Northeast Brazil, and that aqueous extract of Moringa oleifera and PPy-NPs show bactericidal activity against these isolates.

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“…3,4 Uma lectina solúvel em água isolada das sementes de M. oleifera (WSMoL) tem demonstrado atividade inseticida, [5][6][7] e estudos in vitro demonstraram sua atividade antibacteriana contra bactérias corrosivas e patogênicas. [8][9][10] A WSMoL demonstrou atividade anti-inflamatória in vitro em macrófagos murinos estimulados por lipopolissacarídeos, 11 e foi capaz de ativar linfócitos humanos a partir de culturas de células mononucleares do sangue periférico, mostrando um efeito imunomodulador. 12 Também tem sido comprovado que WSMoL é uma das proteínas coagulantes encontradas nas sementes de M. oleifera, 8,13 e é capaz de reduzir a turbidez e a ecotoxicidade de amostras de água coletadas de um riacho poluído.…”

Section: Introductionunclassified

Avaliação dos Efeitos Cardíacos de Lectina Solúvel em Água (WSMoL) de Sementes de Moringa Oleifera

et al. 2020

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ResumoFundsamento: As sementes de Moringa oleifera, que são utilizadas para clarificação de água, contêm uma lectina chamada WSMoL que tem mostrado atividade antibacteriana e imunomoduladora in vitro. Devido ao seu valor nutritivo e potencial terapêutico, as folhas e as sementes dessa árvore são consumidas em algumas comunidades. Algumas lectinas de plantas não são tóxicas para mamíferos, mas tem sido relatado que outras são prejudiciais quando ingeridas ou administradas por outros meios.Objetivo: Como um dos passos necessários para determinar a segurança de WSMoL, nós avaliamos os possíveis efeitos cardiotóxicos desta proteína purificada.Métodos: Durante 21 dias consecutivos, a WSMoL foi administrada a camundongos por gavagem. Foram investigadas as funções eletrofisiológicas, mecânicas e metabólicas in vivo e ex vivo por meio de registros eletrocardiográficos, ressonância magnética nuclear e respirometria de alta resolução. Resultados: O tratamento com WSMoL não induziu alterações nos níveis de glicose no sangue ou peso corporal emcomparação com o grupo controle. Adicionalmente, as relações peso cardíaco/peso corporal e peso cardíaco/comprimento tibial estavam semelhantes em ambos os grupos. A ingestão de lectina também não modificou a tolerância à glicose ou resistência à insulina. Não foram observadas alterações nos parâmetros eletrocardiográficos ou na duração do potencial de ação cardíaco. Os corações dos camundongos dos grupos controle e WSMoL mostraram função ventricular esquerda preservada. Além disso, a WSMoL não induziu alterações na função mitocondrial (em todos os casos, p > 0,05). Conclusões: A administração de WSMoL demonstrou ter um perfil de segurança cardíaca. Estes resultados contribuem à avaliação de segurança do uso de sementes de M. oleifera para tratar água, visto que essa lectina está presente na preparação empregada por algumas populações com esse fim. (Arq Bras Cardiol. 2020; [online].ahead print, PP.0-0) Palavras-chave: Moringa Oleifera; Lectinas; Glicosídeos; Carboidratos; Coração; Segurança Hídrica; Camundongos. AbstractBackground: Moringa oleifera seeds, which are used for water clarification, contain a lectin named WSMoL which has shown in vitro antibacterial and immunomodulatory activity. Due to their nutritional value and therapeutic potential, the leaves and seeds of this tree are eaten in some communities. Some plant lectins are non-toxic to mammals, but others have been reported to be harmful when ingested or administered by other means.Objective: As one of the steps needed to define the safety of WSMoL, we evaluated possible cardiotoxic effects of this purified protein.Methods: WSMoL was administered for 21 consecutive days to mice by gavage. Electrophysiological, mechanical, and metabolic cardiac functions were investigated by in vivo and ex vivo electrocardiographic recordings, nuclear magnetic resonance, and high-resolution respirometry. Results:The treatment with WSMoL did not induce changes in blood glucose levels or body weight in comparison with control group. Moreover, ...

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Multi-effect of the water-solubleMoringa oleiferalectin againstSerratia marcescensandBacillussp.: antibacterial, antibiofilm and anti-adhesive properties

Abstract: Taking together, our results provide support to the potential use of WSMoL for controlling biofilm formation by bacteria.

Cited by 48 publications

References 48 publications

Structure-function and application of plant lectins in disease biology and immunity

Structure-function and application of plant lectins in disease biology and immunity

Antimicrobial activity of polypyrrole nanoparticles and aqueous extract ofMoringa oleiferaagainstStaphylococcusspp. carriers of multi-drug efflux system genes isolated from dairy farms

Avaliação dos Efeitos Cardíacos de Lectina Solúvel em Água (WSMoL) de Sementes de Moringa Oleifera

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