Multi-layered macroporous three-dimensional nanofibrous scaffold via a novel gas foaming technique

Joshi, Mahesh Kumar; Pant, Hem Raj; Tiwari, Arjun Prasad; Kim, Han Joo; Park, Chan Hee; Kim, Cheol Sang

doi:10.1016/j.cej.2015.03.121

Cited by 111 publications

(59 citation statements)

References 39 publications

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“…(B) Bar graph showing the Young's modulus for different mats; values were expressed as mean ± S.E.M. (*, # P <0.05 and **, ## P <0.01; One-way ANOVA post hoc Tukey test)Scaffolds must provide and retain sufficient mechanical support during cell proliferation and tissue regeneration without new-tissue deformation 44. Therefore, scaffold mechanical properties were evaluated.…”

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confidence: 99%

In Situ Generation of Cellulose Nanocrystals in Polycaprolactone Nanofibers: Effects on Crystallinity, Mechanical Strength, Biocompatibility, and Biomimetic Mineralization

Joshi

Tiwari

Pant³

et al. 2015

ACS Appl. Mater. Interfaces

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139

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Post-electrospinning treatment is a facile process to improve the properties of electrospun nanofibers for various applications. This technique is commonly used when direct electrospinning is not a suitable option to fabricate a nonwoven membrane of the desired polymer in a preferred morphology. In this study, a representative natural-synthetic hybrid of cellulose acetate (CA) and polycaprolactone (PCL) in different ratios was fabricated using an electrospinning process, and CA in the hybrid fiber was transformed into cellulose (CL) by post-electrospinning treatment via alkaline saponification. Scanning electron microscopy was employed to study the effects of polymer composition and subsequent saponification on the morphology of the nanofibers. Increasing the PCL content in the PCL/CA blend solution caused a gradual decrease in viscosity, resulting in smoother and more uniform fibers. The saponification of fibers lead to pronounced changes in the physicochemical properties. The crystallinity of the PCL in the composite fiber was varied according to the composition of the component polymers. The water contact angle was considerably decreased (from 124° to less than 20°), and the mechanical properties were greatly enhanced (Young's Modulus was improved by ≈20-30 fold, tensile strength by 3-4 fold, and tensile stress by ≈2-4 fold) compared to those of PCL and PCL/CA membranes. Regeneration of cellulose chains in the nanofibers increased the number of hydroxyl groups, which increased the hydrogen bonding, thereby improving the mechanical properties and wettability of the composite nanofibers. The improved wettability and presence of surface functional groups enhanced the ability to nucleate bioactive calcium phosphate crystals throughout the matrix when exposed to a simulated body fluid solution. Experimental results of cell viability assay, confocal microscopy, and scanning electron microscopy imaging showed that the fabricated nanofibrous membranes have excellent ability for MC3T3-E1 cell proliferation and growth. Given the versatility and widespread use of cellulose-synthetic hybrid systems in the construction of tissue-engineered scaffolds, this work provides a novel strategy to fabricate the biopolymer-based materials for applications in tissue engineering and regenerative medicine.

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confidence: 99%

In Situ Generation of Cellulose Nanocrystals in Polycaprolactone Nanofibers: Effects on Crystallinity, Mechanical Strength, Biocompatibility, and Biomimetic Mineralization

Joshi

Tiwari

Pant³

et al. 2015

ACS Appl. Mater. Interfaces

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139

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“…Typhimurium, H. alvei and E. cloacae; all responsible for nosocomial infections that can lead to lifethreatening bacteremia (Alexopoulou et al, 2016;Kim et al, 2015;Steinbach, Töpper, Adam, & Kees, 2015).…”

Section: Results and Discussion 31 Release From Aerogels And Activitmentioning

confidence: 99%

“…where P is the applied pressure, r is the radius of the pore, σ is the surface tension of the mercury and θ is the contact angle between mercury and polymer. A surface tension of 480 mN/m and a contact angle of 140° (Kim & Chu, 2000;Joshi et al, 2015) were used for all measurements.…”

Section: Determination Of Pore Sizementioning

confidence: 99%

“…A similar experiment was carried out on a strain of S. marcescens, a species recognized to cause human clinical diseases, with a multidrug-resistance profile (Zavascki, Goldani, Li & Nation, 2007). Following the same protocol as above, we have evaluated four other Gram-negative bacterial species involved in nosocomial infections, that can be fatal, mostly if infected patients are elderly, young, or people with depressed immune systems (Alexopoulou et al, 2016;Kim et al, 2015;Steinbach et al, 2015). The bacterial strains were the following: S. Typhimurium (Fig.…”

Section: S In "Supplementarymentioning

confidence: 99%

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“Aerogels of enzymatically oxidized galactomannans from leguminous plants: Versatile delivery systems of antimicrobial peptides and enzymes”

Campia

Ponzini

Rossi

et al. 2017

Carbohydrate Polymers

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We describe aerogels obtained by laccase/TEMPO-oxidation and lyophilization of galactomannans (GM) from fenugreek, sesbania and guar. Enzymatic oxidation of GM in aqueous solution caused viscosity increase up to fifteen-fold, generating structured, elastic, stable hydrogels, presumably due to establishment of hemiacetalic bonds between newly formed carbonyl groups and free hydroxyl groups. Upon lyophilization, water-insoluble aerogels were obtained, whose mechanical properties and porosity were investigated. Active principles were absorbed into the aerogels from aqueous solutions and, following rinsing, blotting, re-lyophilization, were released in an appropriate medium. The release of the antibiotic polymyxin B was tested against six different Gram-negative bacterial strains, of the antimicrobial peptide nisin against two Gram-positive and of the muraminidase lysozyme against one anaerobic strain. Protease and lipase release in solution from "enzyme loaded" aerogels was monitored by the increase in enzymatic activity. These biomaterials could represent new versatile, biocompatible "delivery systems" of actives for biomedical and industrial applications.

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“…To overcome these deficiencies several methods, such as a direct-write electrospinning [11] and multi-layered nanofiber mat [12], have been suggested. Here we will prepare thickness-controllable porous cross-linked PCL (c-PCL) membranes, which are expected to have sufficient porosity, improved pore interconnectivity, controllable and high mechanical properties, by using dual pore-forming agents and adjusting the ratios of the agents.…”

Section: Introductionmentioning

confidence: 99%

A convenient method to fabricate porous cross-linked PCL membrane by using dual pore-forming agents

Yin

Zhang

2016

Materials Letters

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Multi-layered macroporous three-dimensional nanofibrous scaffold via a novel gas foaming technique

Cited by 111 publications

References 39 publications

In Situ Generation of Cellulose Nanocrystals in Polycaprolactone Nanofibers: Effects on Crystallinity, Mechanical Strength, Biocompatibility, and Biomimetic Mineralization

In Situ Generation of Cellulose Nanocrystals in Polycaprolactone Nanofibers: Effects on Crystallinity, Mechanical Strength, Biocompatibility, and Biomimetic Mineralization

“Aerogels of enzymatically oxidized galactomannans from leguminous plants: Versatile delivery systems of antimicrobial peptides and enzymes”

A convenient method to fabricate porous cross-linked PCL membrane by using dual pore-forming agents

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