Multi-locus sequence typing and antimicrobial susceptibility profile of Cronobacter sakazakii and Cronobacter malonaticus isolated from corn-based farinaceous foods commercialized in Brazil

Costa, Paula Vasconcelos; Vasconcellos, Luiza; Silva, Ingrid Camelo da; Medeiros, Valéria de Mello; Forsythe, Stephen J.; Brandão, Marcelo Luiz Lima

doi:10.1016/j.foodres.2019.108805

Cited by 15 publications

(11 citation statements)

References 27 publications

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“…Similarly, Cronobacter isolates were found to be resistant to meropenem in spices and cereals (25.0%) in our previous study (Li et al., 2017), while no meropenem‐resistant strains were found infant formula, drinking water, and food samples in China (Fei et al., 2018). The resistance to tetracycline was previously reported for Cronobacter isolates for other retail products, such as retail foods (3.8%) analyzed in the Czech Republic (Vojkovska et al., 2016), corn‐based farinaceous foods (5.0%) from Brazil (Costa et al., 2020), aquatic products (6.1%) from China (Costa et al., 2020), and indigenous infant flours (43.7%) from Côte d'Ivoire (Yao et al., 2016). Although the antimicrobial resistance of Cronobacter spp.…”

Section: Resultsmentioning

confidence: 62%

“…These results are consistent with previous findings that C. sakazakii is the most dominant species of Cronobacter spp. isolated from different sources (Brandao et al, 2017;Costa et al, 2020Costa et al, , 2021Pei et al, 2019;Wang et al, 2019). MLST revealed 20 STs from the 31 Cronobacter isolates including two newly identified STs, suggesting extremely high genetic diversity of Cronobacter spp.…”

Section: Discussionmentioning

confidence: 93%

“…These results are consistent with previous findings that C. sakazakii is the most dominant species of Cronobacter spp. isolated from different sources (Brandao et al., 2017; Costa et al., 2020, 2021; Pei et al., 2019; Wang et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

“…in dried edible mushrooms. Previous genotyping studies of Cronobacter strains recovered from a variety of food sources also showed a high degree of genetic diversity of Cronobacter species (Costa et al, 2020;Fei et al, 2017;Zeng et al, 2020). In addition, new Cronobacter STs are more likely to be identified in different sources in the near future, some of which may cause human infections, indicating a continuous challenge for controlling the Cronobacter contamination in the food industry.…”

Section: Discussionmentioning

confidence: 99%

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Identification and antibiotic resistance of Cronobacter spp. isolated from dried edible mushrooms

Jiang

Xiang

et al. 2022

Journal of Food Science

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Cronobacter spp. is an important foodborne pathogen that can cause life-threatening diseases in infants and immunocompromised adults. The present study was carried out to understand the prevalence and characterization of Cronobacter spp. in dried edible mushrooms in Jiangsu province, China. Cronobacter isolates were identified and genotyped by multilocus sequence typing (MLST); the antimicrobial susceptibility of Cronobacter strains was determined by the disk diffusion method; the biofilm formation ability of Cronobacter spp. was assessed using the microtiter plate method. The overall prevalence of Cronobacter spp. in dried edible mushrooms was 14.8%, with the highest contamination rate of after 37.2% found in Auricularia auricular. The Cronobacter isolates were identified as C. sakazakii (n = 26), C. malonaticus (n = 2), C. dublinensis (n = 2) and C. turicensis (n = 1). The MLST scheme produced 20 sequence types (STs), two of which were newly identified. ST148 was the most prevalent ST (n = 5), followed by ST4 (n = 3), ST17 (n = 3), ST64 (n = 3), and ST540 (n = 2). One (3.2%) and 15 (48.4%) Cronobacter isolates were resistant to tetracycline and meropenem, respectively. In contrast, all of the tested isolates were susceptible to the remaining 14 antibiotics. Moreover, 20 (64.5%) Cronobacter isolates showed weak ability to produce biofilm, but no isolates showed strong or moderate biofilm-forming ability.

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Section: Resultsmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification and antibiotic resistance of Cronobacter spp. isolated from dried edible mushrooms

Jiang

Xiang

et al. 2022

Journal of Food Science

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“…Breast milk is usually the most important source of nutrition for newborns and is essential for survival, immunological development, health, and early growth in life ( Baker et al , 2021 ). Alternatively, non-breast-fed newborns can be fed on commercial powdered infant milk formula (IMF) that provides a nutritious alternative to breast milk ( Costa et al , 2020 ). Infant formula is considered to be a microbiologically high-quality product, however, some microorganisms have been identified in neonatal infections associated with infant formula feeding ( Garbaj et al , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula

Garbaj¹,

Farag²,

Sherif³

et al. 2023

Open Vet J

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Background: Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These pathogens have been incriminated in sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate. Aim: The current study was performed to demonstrate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF). Methods: The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48, 52, 56, 60, 64 and 66°C. The D- and z-values were determined by using linear regression analysis. Results: The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5 and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C ranged from 7.29 ˗ 23.47, 2.77˗15.50, 0.62˗1.04, 0.62˗1.02, 0.62˗1.00, 0.62˗1.00 min, respectively, while the z˗ values extended from 2.50˗4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) ranged from 7.60 ˗ 22.32, 1.42 ˗ 8.45, 0.62 ˗ 1.08, 0.62 ˗ 0.78, 0.62 ˗ 0.78, 0.62 ˗ 0.79 min. at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33 ˗ 4.89°C. Conclusion: This study may contribute to improve the understanding of behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperature and may participate in effective control of this pathogens in infant’s foods production.

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The changes of antioxidant system and intestinal bacteria in earthworms (Metaphire guillelmi) on the enhanced degradation of tetracycline

et al. 2021

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Multi-locus sequence typing and antimicrobial susceptibility profile of Cronobacter sakazakii and Cronobacter malonaticus isolated from corn-based farinaceous foods commercialized in Brazil

Cited by 15 publications

References 27 publications

Identification and antibiotic resistance of Cronobacter spp. isolated from dried edible mushrooms

Identification and antibiotic resistance of Cronobacter spp. isolated from dried edible mushrooms

Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula

The changes of antioxidant system and intestinal bacteria in earthworms (Metaphire guillelmi) on the enhanced degradation of tetracycline

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