To date, there has been little research attention paid to jetting deformation and pinching-off of microfluidic flows induced by the surface acoustic wave (SAW) mechanism. Further, such studies were almost limited to one sessile drop actuation without any confinement mechanisms. Such a scenario is likely attributable to the mechanism’s relatively poor controllability, the difficulty of maintaining the fluid loading position and issues related to stability and repeatability. In this paper, a novel SAW-microfluidic jetting system with a vertical capillary tube was designed, accompanied by a large number of experiments investigating the single droplet jetting mechanism with different device dimensions, resonance frequencies and radio frequency (RF) power capabilities. The study began with the whole jetting deformation and droplet pinching off through the use of a microscope with a high-speed camera, after which the results were discussed to explain the droplet jetting mechanism in a vertical capillary tube. After that, the study continued with experimental and theoretical examinations for high-quality single droplet jetting conditions. Jetting characterization parameters, including threshold RF power, resonance frequency, liquid volume, pinching off droplet dimensions, were thoroughly analyzed. Lastly, the Weber number range, a significant parameter in SAW-microfluidic jetting, was verified, and the pinching off microdroplet dimension was analyzed and compared via experiments. The significance of this study lies in the realization of microfluidic drop-on-demand based on SAW technology.