Multi-omics analysis: Repeated exposure of a 3D bronchial tissue culture to whole-cigarette smoke

Ishikawa, Shinkichi; Matsumura, Kazunori; Kitamura, Nobumasa; Takanami, Yuichiro; Ito, Shigeaki

doi:10.1016/j.tiv.2018.10.001

Cited by 30 publications

(26 citation statements)

References 55 publications

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“…The analysis of the CTD database revealed that expression of CTSL and CTSV is increased upon cigarette smoke exposure in bronchial epithelial cells and monocytes and CTSV expression is also increased in 3D-bronchial tissues ( Anthérieu et al, 2017 ; Ishikawa et al, 2019 ; Wright et al, 2012 ). We thus further investigated the level of expression of the different genes in the respiratory tract tissues of smokers, nonsmokers and former smokers.…”

Section: Resultsmentioning

confidence: 99%

Assessing the consequences of environmental exposures on the expression of the human receptor and proteases involved in SARS-CoV-2 cell-entry

Watzky

Dieuleveult

Letessier

et al. 2021

Environmental Research

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The role of environmental condition on the infection by the novel pathogenic SARS-CoV-2 virus remains uncertain. In here, exploiting a large panel of publicly available genome-wide data, we investigated whether the human receptor ACE2 and human proteases TMPRSS2 , FURIN and CATHEPSINs (B, L and V), which are involved in SARS-CoV-2 cell entry, are transcriptionally regulated by environmental cues. We report that more than 50 chemicals modulate the expression of ACE2 or human proteases important for SARS-CoV-2 cell entry. We further demonstrate that transcription factor AhR, which is commonly activated by pollutants, binds to the promoter of TMPRSS2 and enhancers and/or promoters of Cathepsin B, L and V encoding genes. Our exploratory study documents an influence of environmental exposures on the expression of genes involved in SARS-CoV-2 cell entry. These results could be conceptually and medically relevant to our understanding of the COVID-19 disease, and should be further explored in laboratory and epidemiologic studies.

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Section: Resultsmentioning

confidence: 99%

Assessing the consequences of environmental exposures on the expression of the human receptor and proteases involved in SARS-CoV-2 cell-entry

Watzky

Dieuleveult

Letessier

et al. 2021

Environmental Research

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“…Metabolomic analysis of underlying tissue samples may reveal slight changes of biochemical profile prior to histopathology [45]. An in vivo study found energy production pathways in lung tissue disturbed after whole-CS exposure [46]. Another metabolomics study indicated that the disturbance of lipid metabolism in lung tissue was associated with the occurrence and development of COPD to a large extent, which could be improved by aminophylline therapy [47].…”

Section: Copd-associated Biomarkers In Different Sample Types and mentioning

confidence: 99%

“…Elevated energy metabolism due to nutritional supplementation significantly improves COPD symptoms [84]. Metabolic pathways correlated with energy production, such as glycolysis, pentose phosphate pathway (PPP), and the TCA cycle, are perturbed in COPD according to some metabolomics studies [46].…”

Section: The Role Of Metabolism Dysfunction In Copd Pathogenesismentioning

confidence: 99%

An Updated Overview of Metabolomic Profile Changes in Chronic Obstructive Pulmonary Disease

Ran

Pang

et al. 2019

Metabolites

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Chronic obstructive pulmonary disease (COPD), a common and heterogeneous respiratory disease, is characterized by persistent and incompletely reversible airflow limitation. Metabolomics is applied to analyze the difference of metabolic profile based on the low-molecular-weight metabolites (<1 kDa). Emerging metabolomic analysis may provide insights into the pathogenesis and diagnosis of COPD. This review aims to summarize the alteration of metabolites in blood/serum/plasma, urine, exhaled breath condensate, lung tissue samples, etc. from COPD individuals, thereby uncovering the potential pathogenesis of COPD according to the perturbed metabolic pathways. Metabolomic researches have indicated that the dysfunctions of amino acid metabolism, lipid metabolism, energy production pathways, and the imbalance of oxidations and antioxidations might lead to local and systematic inflammation by activating the Nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway and releasing inflammatory cytokines, like interleutin-6 (IL-6), tumor necrosis factor-α, and IL-8. In addition, they might cause protein malnutrition and oxidative stress and contribute to the development and exacerbation of COPD.

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“…[13][14][15] The metabolomics technique has been widely applied in mechanism studies and biomarker screening using exhaled breath condensate 16 or blood of COPD patients. 17 A variety of metabolic pathways and biological molecules have been found to be involved in the progression of COPD, such as amino acid metabolism dysfunction, [18][19][20] oxidative stress, 21,22 energy metabolism dysfunction, 23,24 and lipid metabolism dysfunction. 25,26 But most of the previous studies focused on patients from African, American, and European populations, and to the best of our knowledge, no COPD related metabolomics study has been reported using large cohorts of the Chinese population.…”

Section: Introductionmentioning

confidence: 99%

<p>Plasma Metabolomics and Lipidomics Reveal Perturbed Metabolites in Different Disease Stages of Chronic Obstructive Pulmonary Disease</p>

Zhou¹,

Li²,

Liu³

et al. 2020

COPD

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Background: Chronic obstructive pulmonary disease (COPD) is a common disease characterized by persistent respiratory symptoms and airflow restriction. It is usually manifested as airway and/or alveolar abnormalities caused by significant exposure to harmful particulates or gases. Objective: We aim to explore plasma metabolomic changes in the acute exacerbation stage of COPD (AECOPD) and stable stage of COPD (Stable COPD) to identify potential biomarkers for diagnosis or prognosis in clinical practice. Methods: Untargeted metabolomics and lipidomics analyses were performed to investigate dysregulated molecules in blood plasma of AECOPD patients (n=48) and Stable COPD (n=48), and a cohort of healthy people were included as a control group (n=48). Statistical analysis and bioinformatics analysis were performed to reveal dysregulated metabolites and perturbed metabolic pathways. SVM-based multivariate ROC analysis was used for candidate biomarker screening. Results: A total of 142 metabolites and 688 lipids were dysregulated in COPD patients. Pathway enrichment analysis showed that several metabolic pathways were perturbed after COPD onset. Several biomarker panels were proposed for diagnosis of COPD vs healthy control and AECOPD vs Stable COPD with AUC greater than 0.9. Conclusion: Numerous plasma metabolites and several metabolic pathways were detected relevant to COPD disease onset or progression. These metabolites may be considered as candidate biomarkers for diagnosis or prognosis of COPD. The perturbed pathways involved in COPD provide clues for further pathological mechanism studies of COPD.

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Multi-omics analysis: Repeated exposure of a 3D bronchial tissue culture to whole-cigarette smoke

Cited by 30 publications

References 55 publications

Assessing the consequences of environmental exposures on the expression of the human receptor and proteases involved in SARS-CoV-2 cell-entry

Assessing the consequences of environmental exposures on the expression of the human receptor and proteases involved in SARS-CoV-2 cell-entry

An Updated Overview of Metabolomic Profile Changes in Chronic Obstructive Pulmonary Disease

<p>Plasma Metabolomics and Lipidomics Reveal Perturbed Metabolites in Different Disease Stages of Chronic Obstructive Pulmonary Disease</p>

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