Multi-omics based strategy for toxicity analysis of acrylamide in Saccharomyces cerevisiae model

Lin, Zhen; Luo, Ping; Huang, Dongmin; Wu, Yulian; Li, Fangping; Liu, Huazhong

doi:10.1016/j.cbi.2021.109682

Cited by 8 publications

(2 citation statements)

References 40 publications

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“…Acrylamide is a serious threat to human health [1,[4][5][6][7][8]. Various studies reported the negative impact for acrylamide on health through the generation of oxidative stress together with neurotoxicity, reproductive, and hepatorenal toxicity [1,3,5,9,10]. It decreases the antioxidant capacity through its deleterious impacts on the endogenous antioxidant enzymes [11,12].…”

Section: Introductionmentioning

confidence: 99%

Taify Pomegranate Juice Extract Abrogates Testicular Dysfunction Induced by Acrylamide: Role of Inflammatory, Antioxidants, and Oxidative Stress Biomarkers

Soliman,

Sayed,

Youssef

et al. 2023

Andrologia

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High altitude (HA) poses various dangers to living organisms, the most serious of which are oxidative stress and its accompanying metabolic problems, as well as hypoxia and its associated metabolic abnormalities. Acrylamide is a poisonous chemical produced under the oxidative stress as a result of intracellular reactive oxygen species formation and toxicity. Heating carbohydrates-rich meals give acrylamide that is widely used in the industry. The precise mechanism of acrylamide toxicity is unknown. The current study aimed to examine the impacts of Taify pomegranate juice (TPJ) from Taif area (Punica granatum L.) on acrylamide-induced testicular stress and dysfunction. Twenty four male of adult Wistar rats, were divided into four groups: Group 1 was a negative-control received only saline; Group 2 was a positive-control that received orally acrylamide (20 mg/kg/bw) for consecutive 4 weeks; and Group 3 received TPJ; 2 mL/kg/bw, orally for 4 weeks. Rats in Group 4 received pomegranate juice and acrylamide as described in Groups 2 and 3, with the TPJ delivered 1 week before the acrylamide. Acrylamide elevated serum levels of inflammatory cytokines (interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α)). Moreover, acrylamide decreased concentrations of follicle stimulating hormone (FSH), leutinizing hormone (LH), and testosterone and altered semen characteristics (decreased sperm viability and concentration, and increased sperm abnormalities). Acrylamide increased malondialdehyde levels (MDA), while it decreased antioxidant activities (superoxide dismutase; SOD and reduced glutathione; GSH). The inflammation associated cytokines were restored by TPJ administration to acrylamide rats. GSH and SOD were significantly recovered to near control levels in TPJ plus acrylamide group compared to acrylamide-administered rats. TPJ preadministration to rats restored semen profiles, alteration in testis pathology, and normalized the changes on the male reproductive hormones affected by acrylamide. Furthermore, the TPJ reversed the upregulation in caspases-3 and the decrease in B-cell lymphoma-2 (Bcl-2) gene expressions affected by acrylamide, with significant upregulation of hemoxygenase-1 (HO-1) and nuclear factor erythroid-2 (Nrf2) mRNA expression. These findings collectively revealed that TPJ possesses anti-inflammatory, potent antioxidant, and antiapoptotic effect against acrylamide-induced testicular damage.

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Section: Introductionmentioning

confidence: 99%

Taify Pomegranate Juice Extract Abrogates Testicular Dysfunction Induced by Acrylamide: Role of Inflammatory, Antioxidants, and Oxidative Stress Biomarkers

Soliman,

Sayed,

Youssef

et al. 2023

Andrologia

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“…It was declared a carcinogenic agent in 1994 by The International Agency for Research on Cancer ( 3 ). Various studies report the wide range of ACR's negative impact on health, including oxidative stress and cancer ( 1 , 4 – 8 ), neurotoxicity ( 1 , 3 , 4 , 9 , 10 ), reproductive toxicity ( 1 , 4 , 11 ), and developmental toxicity ( 1 , 12 ). Acrylamide exerts its harmful effects by increasing the level of reactive oxygen species (ROS) and decreasing the antioxidant capacity via its deleterious effects to the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) ( 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

Taify Pomegranate Juice (TPJ) Abrogates Acrylamide-Induced Oxidative Stress Through the Regulation of Antioxidant Activity, Inflammation, and Apoptosis-Associated Genes

et al. 2022

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Acrylamide (ACR) has various effects on biological systems, including oxidative stress and its associated metabolic disorders. Previous research reports that plants growing at high altitude have a different profile of antioxidants. In the current report, the Taify pomegranate juice (TPJ) of the Taify pomegranate growing at the Taif region (high altitude), Saudi Arabia, was investigated for its protective activity from ACR-induced oxidative stress. Rats were treated with ACR, TPJ, or TPJ+ACR, and various assays, including blood chemistry, liver function biomarkers, gene expression of endogenous antioxidant enzymes, oxidative stress regulatory genes, inflammation biomarkers, and apoptosis, were estimated using biochemical, real-time PCR, histopathological, and immunohistochemical analysis. TPJ showed a protective function of ACR-induced alteration of AST, ALT, GGT, urea, total proteins, albumin, MDA, and NO. It also increased the level of the endogenous antioxidative enzymes, including SOD, catalase, and GSH. It showed anti-inflammatory activity by reduction the TNF-α, IL-6 secretion and the enhancing of IL-10 levels. At the gene expression level, TPJ upregulated the expression of endogenous antioxidant genes (SOD and catalase) and of antioxidant-regulating genes Nrf2 and HO-1; downregulated the expression of inflammatory genes TGF-β1, COX2, and the apoptotic gene caspase-3; and upregulated the expression of antiapoptotic gene Bcl2. At the histological level, TPJ showed a protective effect from the ACR-induced hepatic histological damage. Results of this study conclude that TPJ has a protective effect from ACR-induced oxidative stress and its associated metabolic alterations through its antioxidant and anti-inflammatory activities.

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Metabolome analysis of the response and tolerance mechanisms of Saccharomyces cerevisiae to formic acid stress

Zeng

Zhao

et al. 2022

The International Journal of Biochemistry & Cell Biology

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Multi-omics based strategy for toxicity analysis of acrylamide in Saccharomyces cerevisiae model

Cited by 8 publications

References 40 publications

Taify Pomegranate Juice Extract Abrogates Testicular Dysfunction Induced by Acrylamide: Role of Inflammatory, Antioxidants, and Oxidative Stress Biomarkers

Taify Pomegranate Juice Extract Abrogates Testicular Dysfunction Induced by Acrylamide: Role of Inflammatory, Antioxidants, and Oxidative Stress Biomarkers

Taify Pomegranate Juice (TPJ) Abrogates Acrylamide-Induced Oxidative Stress Through the Regulation of Antioxidant Activity, Inflammation, and Apoptosis-Associated Genes

Metabolome analysis of the response and tolerance mechanisms of Saccharomyces cerevisiae to formic acid stress

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