Multi-parametric rigid and flexible, low-cost, disposable sensing platforms for biomedical applications

Abstract

The measurement of Na, K and H is essential in medicine and plays an important role in the assessment of tissue ischemia. Microfabrication, inkjet- and screen-printing can be used for solid contact ion selective electrodes (ISE) realization; these, however, can be non-standardized, costly and time consuming processes. We present the realization of ISEs on post-processed electrodes fabricated via standardized printed circuit board (PCB) manufacturing techniques. In vitro results are presented from two rigid platforms (32 ISEs) for liquid sample dip-stick measurements and two flexible platforms (6 and 32 ISEs) for post-surgical intestinal tissue monitoring, each with a common reference electrode (RE). These are combined with optimized tetrapolar bioimpedance sensors for tissue ischemia detection. Both electroless and hard gold PCB finishes are examined. Apart from the electroless rigid platform, the rest demonstrated comparable and superior performance, with the pH sensors demonstrating the greatest deviation; the flexible hard gold platform achieved a sensitivity 4.6mV/pH and 49.2mV/pH greater than the electroless flexible and rigid platforms, respectively. The best overall performance was achieved with the hard gold flexible platform with sensitivities as large as 73.4mV/pH, 56.3mV/log [Na], and 57.4mV/log [K] vs. custom REs on the same substrate. Simultaneous measurement of target analytes is demonstrated with test solutions and saliva samples. The results demonstrate superior performance to other PCB-based pH sensors and Na and K PCB-based sensors with comparable performance to potentiometric sensors fabricated with other techniques, paving the way towards mass-produced, low-cost, disposable, multi-parametric chemical sensing diagnostic platforms.

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

“…The sharp increase in development and testing of new biomaterials in recent years means that there is a real need for higher throughput screening systems in the biomedical field to accelerate the development of new and improved implant biomaterials with lower costs [1][2][3][4]. Conventional in vitro cell culture experimental set-ups are used in early stages of development to characterize cell culture/biomaterials interactions.…”

Bioimpedance Sensors: A Tutorial

“…Alternatively, if this is not the case or possible, additional sensors must be used to corroborate impedance measurements. One example is tissue ischemia measurements where measurement of tissue pH, Na + , K + , lactate and glucose, as well as near infrared spectroscopy (NIRS) can be used in conjunction with bioimpedance measurements [133]- [135]. These are all non-specific indirect markers or ischemia, but together they provide a holistic analysis of tissue dynamics.…”

The review of Lab‐on‐PCB for biomedical application

“…The pictures of these platforms are shown in Fig. 6; they are divided into two rigid platforms for in vitro liquid sample measurement and two flexible platforms for postoperative intestinal tissue testing [59]. To demonstrate their detection performance, the detection results of the four platforms .…”