2022
DOI: 10.3390/ijms23031575
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Multi Platforms Strategies and Metabolomics Approaches for the Investigation of Comprehensive Metabolite Profile in Dogs with Babesia canis Infection

Abstract: Canine babesiosis is an important tick-borne disease worldwide, caused by parasites of the Babesia genus. Although the disease process primarily affects erythrocytes, it may also have multisystemic consequences. The goal of this study was to explore and characterize the serum metabolome, by identifying potential metabolites and metabolic pathways in dogs naturally infected with Babesia canis using liquid and gas chromatography coupled to mass spectrometry. The study included 12 dogs naturally infected with B. … Show more

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Cited by 10 publications
(4 citation statements)
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“…Once the stent comes into contact with blood, blood proteins adhere to the stent's surface, causing a protein layer to develop. One of the blood proteins, fibrinogen, may cause platelet activation, aggregation, and the start of the coagulation cascade (Rubic et al, 2022). BSA has been widely used in biomedical research due to its comparability and validation with existing literature and findings (Ribeiro et al, 2022).…”
Section: Discussionmentioning
confidence: 99%
“…Once the stent comes into contact with blood, blood proteins adhere to the stent's surface, causing a protein layer to develop. One of the blood proteins, fibrinogen, may cause platelet activation, aggregation, and the start of the coagulation cascade (Rubic et al, 2022). BSA has been widely used in biomedical research due to its comparability and validation with existing literature and findings (Ribeiro et al, 2022).…”
Section: Discussionmentioning
confidence: 99%
“…The combination of these indicators indicates a systemic inflammatory response of the dog's organism to the destructive influence of Babesia spp. (Kirtz et al, 2012;Goddard et al, 2016;Rubić et al, 2022).…”
Section: Discussionmentioning
confidence: 99%
“…Sample preparation and metabolite extraction were performed according to the manufacturer instructions provided with the kit and described in detail previously [25]. In short, the 10 μL of each sample, calibration standards, blank samples (phosphate-buffered saline; BDH PROLABO, Lutterworth, UK), and three quality control samples (low, QC1; medium, QC2 and high, QC3) were prepared on the specific 96-well plate for protein removal, internal standard normalisation and derivatization.…”
Section: Targeted Metabolomics Analysismentioning
confidence: 99%