Multi-Residue Analysis of Organophosphorus Pesticides in Vegetable Using GC-MS

Abstract

This study investigates the levels of pesticide residues in Cucumbers (Cucumis sativus), potatoes (Solanum tuberosum subsp. Tuberosum) and tomatoes (Lycopersicon esculentum). The samples were minced in a food processor initially subjected to extraction followed by cleanup using solid phase extraction (SPE) column. Separation was performed on a GC capillary column (Rtx-5 ms-30 m × 0.25 mm × 0.25 µm). The sample was injected by using splitless mode, helium as the carrier gas followed by quadrupole mass spectrometry detection. The mobile phase flow rates, column temperatures, and MS parameters were all optimized to reach high sensitivity and selectivity. Seven pesticides were detected in tomatoes sample, six in cucumber and four in potatoes samples. The findings indicate risks and concerns for public health.

Control of Organophosphorus Pesticides Residues in Honey Samples Using a Miniaturized Tandem Preconcentration Technique Coupled with High Performance Liquid Chromatography

“…In this step, heating temperature and time, salt addition, and centrifuging speed and time can influence analytical signals. This stage was performed using various heating temperature (30, 40, 50, 60, 70, and 80 °C) and time (1,2,3,4,5,7, and 10 min), percent of NaCl (0, 2.5, 5.0, 7.5, and 10%, w/v), and centrifuging speed (3000, 4000, 5000, 6000, and 7000 rpm) and time (3, 4, 5, 6, and 7 min). The optimum conditions were obtained in the absence of salt, 60 °C and 2 min as the heating temperature and time, and 4 min and 5000 rpm as centrifuging time and speed, respectively.…”

“…OPPs can be toxic when they are absorbed by human organisms because of acetyl-cholinesterase deactivation. 1,2 To minimize the risk for human health, food authorities tried to establish regulations such as maximum residue level (MRL) for pesticides including OPPs. To ensure that MRL for certain pesticides are controlled, their residues determination in food is essentially required.…”

“…Therefore, it is essential to provide an efficient method for quantifying harmful pollutants such as pesticides residues in honey samples to warrant consumers' safety. 3,4 Several chromatographic methods such as highperformance liquid chromatography (HPLC), [5][6][7] liquid chromatography-tandem mass spectrometry (LC-MS-MS), 8 and gas chromatography-mass spectrometry (GC-MS) 1,9,10 have been used for OPPs analysis in different samples. Although these methods are sensitive and selective, another stage named as sample preparation is needed before instrumental analysis because of trace levels of pesticides present in the real samples.…”