Multi-species biofilms: how to avoid unfriendly neighbors

Rendueles, Olaya; Ghigo, Jean‐Marc

doi:10.1111/j.1574-6976.2012.00328.x

Cited by 262 publications

(213 citation statements)

References 161 publications

(198 reference statements)

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“…These changes are related to enhancing bacterial surveillance because of disinfectant and antibiotic resistance. Identifying key biofilm determinants in several bacteria is required to achieve preventative strategies in initial bacterial adhesion, maturation of biofilm, or enhancement of antibiotic susceptibility of bacterial community in biofilm (5,6). In the differentiated superficial umbrella cells that line the lumen of the bladder, UPEC are able to break into the host cell cytosol and rapidly multiply, forming large intracellular biofilm-like communities that can contain several thousand bacteria.…”

Section: Introductionmentioning

confidence: 99%

Survey on Biofilm Production and Presence of Attachment Factors in Human Uropathogenic Strains of Escherichia coli

Bakhtiari

Javadmakoei

2017

Jundishapur J Microbiol

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Background: Escherichia coli is one of the most common causes of different infections. Biofilm structure allows the strains to persist on the biotic and abiotic surfaces for a long time and impairs eradication. Surface colonization of E. coli could be done with several extracellular appendages, which are effective productive events leading to biofilm maturity. Objectives: In this study, the possible relationship between the presence of fimA (encoding large subunit of Type I fimbriae) and csgA (encoding curli fimbriae) genes with biofilm formation in extraintestinal pathogen E. coli isolates was evaluated. Methods: For this study, 35 isolates of E. coli were collected from human urine samples of those referred to oil big hospital. After isolating and identifying E. coli strains by common biochemical tests, we examined the biofilm formation of isolates in brain heart infusion broth, which contained 3% sucrose, using microtiter plate crystal violet method. Presence of the 2 studied genes in the isolates was evaluated using multiplex polymerase chain reaction (m-PCR) assay. Results: In the present study, 27 strains from 35 isolates were expressed in the 2 studied attachment-associated factors, but 5 and 2 strains were expressed by csgA and fimA, respectively. Except 2 strains that could not produce biofilm, 1 strain was detected as a moderate biofilm producer, and the 32 remaining strains were detected as weak biofilm producers. Conclusions: All the positive and the 2 negative biofilm producer strains could be expressed in the 2 studied genes. The correlation between the presence of studied genes and biofilm production ability was suspected, but because of the high percentage of biofilm production in the studied strains, the need to use good hygiene practices is highly recommended.

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Section: Introductionmentioning

confidence: 99%

Survey on Biofilm Production and Presence of Attachment Factors in Human Uropathogenic Strains of Escherichia coli

Bakhtiari

Javadmakoei

2017

Jundishapur J Microbiol

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“…De nombreuses interactions ont également été décrites au cours desquelles une bactérie peut utiliser des nutriments produits par des bactéries voisines, conduisant à une complémentarité métabolique très étudiée dans le cadre des processus de bioremédiation [16]. À l'opposé de ces phénomènes coopératifs, les bactéries composant un biofilm peuvent entrer en compétition via la production de molécules inhibant l'implantation d'un organisme exogène dans le biofilm [17]. Enfin, la proximité physique des bactéries favorise le transfert horizontal de matériel génétique par conjugaison ou transformation permettant l'acquisition de gènes de résistance aux biocides ou d'autres caractères bénéfiques augmentant les chances de survie de la communauté [18].…”

Section: Un Haut Lieu De Sociomicrobiologie Et D'échangesunclassified

Infections associées aux biofilms

Lebeaux

Ghigo

2012

Med Sci (Paris)

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> Les biofilms sont des communautés de microorganismes en contact avec une surface. Bien que bénéfiques dans la plupart des environnements, les biofilms bactériens se développant sur des implants ou lors d'infections chroniques constituent des réservoirs de pathogènes à l'origine de nombreuses infections nosocomiales. Malgré la mise en oeuvre de mesures préventives, les biofilms sont difficiles à éradiquer en raison de leur tolé-rance caractéristique à des doses élevées d'antibiotiques. Cette revue présente les progrès récents de la recherche sur la formation, les fonctions originales et le contrôle de ces communautés, qui permettent à présent d'envisager de nouvelles stratégies dirigées contre les biofilms. < Le biofilm : un mode de vie original En dépit de la diversité des situations écologiques, industrielles ou médicales concernées par la formation des biofilms, toutes ces entités biologiques partagent des caractéristiques communes.Un développement dynamique L'adhérence initiale d'une bactérie sur une surface est d'abord conditionnée par ses propriétés physicochimiques (hydrophobie et charge électrostatique). Elle fait ensuite intervenir des macromolécules de l'enveloppe bactérienne -souvent appelées adhésines -qui interagissent de manière spécifique ou non avec les surfaces (Figure 1) [3,7]. Des polysaccharides entrant dans la composition de la matrice extracellulaire assurent également une cohésion mécanique entre les bactéries et contribuent au passage de la « vie libre » à la « vie fixée » [8]. Une petite molécule diffusible, le di-guanosine monophosphate cyclique (di-GMPc), constitue un second messager central régulant la transition entre la vie planctonique et l'établissement d'un biofilm (Figure 2). Sa concentration intracellulaire est influencée par de nombreux stimulus environnementaux et régule l'expression de gènes impliqués dans la formation de biofilm (production de polysaccharides de la matrice) ou bien impliqués dans le phénotype mobile (production de flagelle) [3]. Après leur adhérence au substrat, qui peut survenir dans les secondes qui suivent le contact avec une surface, les bactéries s'agrègent, se

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“…Several studies are published regarding the effect of EOs on food borne pathogens like Listeria. Targeting surface colonization rather than bacterial fitness seems to be a good approach, because the selective pressure is lower and may lead to fewer resistant strains [16]. In a recent study, raw chicken fillets were inoculated with L. monocytogenes and the effects of chitosan (a commonly used preservative) and oregano EO were tested for the extension of shelf life of the meat.…”

Section: Introductionmentioning

confidence: 99%

Anti-listerial effect of selected essential oils and thymol

Kerekes

Vidács

Torok

et al. 2016

Acta Biologica Hungarica

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The anti-listerial effect of marjoram, thyme essential oils (EOs) and thymol on Listeria monocytogenes inoculated chicken breast fillets was investigated. Before inoculation the fillets were pretreated by washing or not under running tap water. Inoculated samples were kept at 6 °C for 24 h to allow the growth of L. monocytogenes. After this, the fillets were put in marinating solutions containing salt (5%) and EOs or thymol in MIC/2 concentration established in vitro. Total germ count (TGC) and L. monocytogenes count was monitored on the meat surface and in the marinating solutions following 24 and 48 h storage at 6 °C. Thyme and thymol reduced significantly Listeria cell count (1-3 log CFU) in both samples. They also gave good flavour to the fried meat. The doses of EOs used were optimal for antimicrobial efficiency and had a pleasant flavour effect. Washing was not efficient in reducing total germ count.

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Multi-species biofilms: how to avoid unfriendly neighbors

Cited by 262 publications

References 161 publications

Survey on Biofilm Production and Presence of Attachment Factors in Human Uropathogenic Strains of Escherichia coli

Survey on Biofilm Production and Presence of Attachment Factors in Human Uropathogenic Strains of Escherichia coli

Infections associées aux biofilms

Anti-listerial effect of selected essential oils and thymol

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