Multi-system Nernst–Michaelis–Menten model applied to bioanodes formed from sewage sludge

Rimboud, Mickaël; Quéméner, Elie Desmond-Le; Erable, Benjamin; Bouchez, Théodore; Bergel, Alain

doi:10.1016/j.biortech.2015.05.069

Cited by 24 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have also shown Ignavibacteria spp. to be relatively abundant in anode biofilms (Yoshizawa et al, 2014;Rimboud et al, 2015;Tian et al, 2015;Baek et al, 2016). Our findings of Ignavibacteria genomes rich in large MHCs and porin-cytochrome gene clusters (Figure 3 and Table 3) add further support for EET by members of this group.…”

Section: Discussionsupporting

confidence: 57%

Diverse Microorganisms in Sediment and Groundwater Are Implicated in Extracellular Redox Processes Based on Genomic Analysis of Bioanode Communities

2020

View full text Add to dashboard Cite

Extracellular electron transfer (EET) between microbes and iron minerals, and syntrophically between species, is a widespread process affecting biogeochemical cycles and microbial ecology. The distribution of this capacity among microbial taxa, and the thermodynamic controls on EET in complex microbial communities, are not fully known. Microbial electrochemical cells (MXCs), in which electrodes serve as the electron acceptor or donor, provide a powerful approach to enrich for organisms capable of EET and to study their metabolism. We used MXCs coupled with genomeresolved metagenomics to investigate the capacity for EET in microorganisms present in a well-studied aquifer near Rifle, CO. Electroactive biofilms were established and maintained for almost 4 years on anodes poised mostly at −0.2 to −0.25 V vs. SHE, a range that mimics the redox potential of iron-oxide minerals, using acetate as the sole carbon source. Here we report the metagenomic characterization of anode-biofilm and planktonic microbial communities from samples collected at timepoints across the study period. From two biofilm and 26 planktonic samples we reconstructed draftquality and near-complete genomes for 84 bacteria and 2 archaea that represent the majority of organisms present. A novel Geobacter sp. with at least 72 putative multiheme c-type cytochromes (MHCs) was the dominant electrode-attached organism. However, a diverse range of other electrode-associated organisms also harbored putative MHCs with at least 10 heme-binding motifs, as well as porin-cytochrome complexes and e-pili, including Actinobacteria, Ignavibacteria, Chloroflexi, Acidobacteria, Firmicutes, Betaand Gammaproteobacteria. Our results identify a small subset of the thousands of organisms previously detected in the Rifle aquifer that may have the potential to mediate mineral redox transformations.

show abstract

Section: Discussionsupporting

confidence: 57%

Diverse Microorganisms in Sediment and Groundwater Are Implicated in Extracellular Redox Processes Based on Genomic Analysis of Bioanode Communities

2020

View full text Add to dashboard Cite

show abstract

“…Cyclic voltammograms were performed after the electrodes reached J max , on day 6. For comparison the theoretical curve, corresponding to the Nernst-Michaelis-Menten kinetics [41,42], was added:…”

Section: Effect Of Micro-roughness On Electroactivity Establishment Amentioning

confidence: 99%

Effect of surface roughness, porosity and roughened micro-pillar structures on the early formation of microbial anodes

Champigneux

Renault-Sentenac

Bourrier

et al. 2019

Bioelectrochemistry

Self Cite

View full text Add to dashboard Cite

The early formation of electroactive biofilms was investigated with gold electrodes inoculated with Geobacter sulfurreducens. Biofilms were formed under an applied potential of 0.1 V/SCE, with a single batch of acetate 10 mM, on flat gold electrodes with different random surface roughness. Roughness with arithmetical mean height (S a) ranging from 0.5 to 6.7 μm decreased the initial latency time, and increased the current density by a factor of 2.7 to 6.7 with respect to nano-rough electrodes (S a = 4.5 nm). The current density increased linearly with S a up to 14.0 A•m −2 for S a of 6.7 μm. This linear relationship remained valid for porous gold. In this case, the biofilm rapidly formed a uniform layer over the pores, so porosity impacted the current only by modifying the roughness of the upper surface. The current density thus reached 14.8 ± 1.1 A•m −2 with S a of 7.6 μm (7 times higher than the nano-rough electrodes). Arrays of 500-μm-high micro-pillars were roughened following the same protocol. In this case, roughening resulted in a modest gain around 1.3-fold. A numerical model showed that the modest enhancement was due to ion transport not being sufficient to mitigate the local acidification of the structure bottom.

show abstract

“…The bioanodes formed at 40 C displayed two clear peaks, centred at around À0.34 and À0.20 V/SCE (solid line, Fig. 4C), and a shoulder around À0.43 V/SCE which can be interpreted as three redox systems being involved in the electron transfer mechanism [67]. In contrast, the bioanodes formed at 25 C showed only the peak centred at À0.35 V/SCE (Fig.…”

Section: à2mentioning

confidence: 87%

Increasing the temperature is a relevant strategy to form microbial anodes intended to work at room temperature

Oliot¹,

Erable²,

Solan³

et al. 2017

Electrochimica Acta

Self Cite

View full text Add to dashboard Cite

OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. Reducing the time required for the formation of microbial anodes from environmental inocula is a great challenge. The possibility of reaching this objective by increasing the temperature during the bioanode preparation was investigated here. Microbial anodes were formed at 25 C and 40 C under controlled potential with successive acetate additions. At 25 C, around 40 days were required to perform three acetate batches, which led to current density of 9.4 ± 2 A.m À2 , while at 40 C, 20 days were sufficient to complete three similar batches, leading to 22.9 ± 4.2 A.m À2 . The bioanodes formed at 40 C revealed three redox systems and those formed at 25 C only one. The temperature also impacted the biofilm structure, which was less compact at 40 C. When the bioanodes formed at 40 C were switched to 25 C, they produced current densities similar to those of bioanodes formed at 25 C; they recovered the single redox system that was developed by the bioanodes formed at 25 C and the difference in biofilm structures was mitigated. It is consequently fully appropriate to accelerate the formation of microbial anodes by increasing the temperatures to 40 C even if they are finally intended to operate at room temperature.

show abstract

Multi-system Nernst–Michaelis–Menten model applied to bioanodes formed from sewage sludge

Cited by 24 publications

References 39 publications

Diverse Microorganisms in Sediment and Groundwater Are Implicated in Extracellular Redox Processes Based on Genomic Analysis of Bioanode Communities

Diverse Microorganisms in Sediment and Groundwater Are Implicated in Extracellular Redox Processes Based on Genomic Analysis of Bioanode Communities

Effect of surface roughness, porosity and roughened micro-pillar structures on the early formation of microbial anodes

Increasing the temperature is a relevant strategy to form microbial anodes intended to work at room temperature

Contact Info

Product

Resources

About