Multi-wavelength spectrophotometric determination of hydrogen peroxide in water with peroxidase-catalyzed oxidation of ABTS

Cai, Huahua; Liu, Xin; Zou, Jing; Xiao, Jingyi; Yuan, Baoling; Li, Fēi; Cheng, Qingfeng

doi:10.1016/j.chemosphere.2017.11.091

Cited by 83 publications

(31 citation statements)

References 41 publications

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“…Oxidation of ABTS TM (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)) is spectrophotometrically recorded at 405 nm [ 29 ]. The arising green coloration is due to the formation of a stable green ABTS + radical ( Figure 16 ) [ 97 ]. Measurement at multiple wavelengths, including at 405 [ 29 ], 415, 650, 732 and 820 nm, is possible with different sensitivities [ 97 ].…”

Section: Analytics Of Aldehydes Used In Biocatalysismentioning

confidence: 99%

Production of Aldehydes by Biocatalysis

Kazimírová

Rebroš

2021

IJMS

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The production of aldehydes, highly reactive and toxic chemicals, brings specific challenges to biocatalytic processes. Absence of natural accumulation of aldehydes in microorganisms has led to a combination of in vitro and in vivo strategies for both, bulk and fine production. Advances in genetic and metabolic engineering and implementation of computational techniques led to the production of various enzymes with special requirements. Cofactor synthesis, post-translational modifications and structure engineering are applied to prepare active enzymes for one-step or cascade reactions. This review presents the highlights in biocatalytical production of aldehydes with the potential to shape future industrial applications.

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Section: Analytics Of Aldehydes Used In Biocatalysismentioning

confidence: 99%

Production of Aldehydes by Biocatalysis

Kazimírová

Rebroš

2021

IJMS

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“…Широкое распространение получила и спектрофотометрия [8][9][10]. В настоящее время разработано значительное количество спектрофотометрических методик определения пероксида водорода, в том числе и твердофазных, с использованием ферментов [11,12] и наночастиц металлов [13][14][15][16], для экспрессного его определения используют тест-методы [10,[17][18][19].…”

Section: Na Gavrilenko 1 *Nv Saranchina 2 Da Fedan 1 Maunclassified

Solid-phase spectrophotometric determination of hydrogen peroxide

Гавриленко¹,

Saranchina²,

Fedan³

et al. 2019

АиК

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Аналитика и контроль. 2019. Т. 23. № 2. absorbance of 365 nm. The amount of iodine extracted by the polymethacrylate matrix was proportional to the concentration of hydrogen peroxide in the solution. The effect of interfering ions was also investigated. The optimal phase contact time was 5 minutes. The effect of adding the hydrochloric acid to the analyzed solution on the analytical signal of the polymethacrylate matrix after its contact with hydrogen peroxide solution was studied. It was shown that the maximum signal was obtained in the range of acid concentrations in the solution of (3-5)•10-3 M. The optimal concentration of potassium iodide in the analyzed solution for the redox reaction was 0.06%. At this concentration, the widest range of detectable concentrations was achieved with the smallest limit of detection for hydrogen peroxide. The developed procedure ensured the determination in the range of (15-130)•10-5 % of hydrogen peroxide with the detection limit of 5×10-5 %. The results of the determination of hydrogen peroxide were given for the proposed method in samples of disinfectants and bleaching agents. The proposed method for hydrogen peroxide determination is more preferable in comparison to the iodometric method [1] as it provides the increased sensitivity, simplicity and rapid analysis, the absence of losses of released iodine due to its solid-phase extraction into the methacrylate polymer and can be conducted using the standard spectrophotometric equipment.

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“…Over the past several decades, many H 2 O 2 sensing techniques have been devised based on spectrophotometry, 14,15 uorescence, 12,16 chemiluminescence, 17 enzymatic, and electrochemical methods. [18][19][20][21][22] One of the most extensively used enzymatic systems for H 2 O 2 sensing is horseradish peroxidase-H 2 O 2 system (HRP-H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 99%

“…[32][33][34][35][36][37][38][39] Most HRP-based methods, however, are limited by some serious disadvantages, such as environmental instability, complex fabrication design, tedious immobilization procedures, and high cost. 15,18 For example, 3,3 0 ,5,5 0tetramethylbenzidine/HRP-based method to detect H 2 O 2 is mainly based on the use of 3,3 0 ,5,5 0 -tetramethylbenzidine (TMB), which is the most commonly used chromogen for HRP. TMB performs as a reducing organic substrate and is oxidized by ferryl intermediates formed upon the reaction of HRP and the H 2 O 2 impurity in the media.…”

Section: Introductionmentioning

confidence: 99%