Multibiomarker-based assessment of toxicity of central European strains of filamentous cyanobacteria Aphanizomenon gracile and Raphidiopsis raciborskii to zebrafish Danio rerio

Falfushynska, Halina; Horyn, Oksana; Osypenko, Inna; Rzymski, Piotr; Wejnerowski, Łukasz; Dziuba, Marcin Krzysztof; Sokolova, Inna M.

doi:10.1016/j.watres.2021.116923

Cited by 20 publications

(20 citation statements)

References 90 publications

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“…Our results suggest that studied BLC cyanobacteria do not produce common cyanotoxins and imply their safe use in restoration. However, with recent developments in analytical techniques and in vitro and in vivo bioassays, bioactive compounds are being discovered that have toxic effects on human and animal cells but are structurally different from the known cyanotoxins, so we should not rule out the presence of some other toxic compounds [ 1 , 73 ]. For example, strains isolated from terrestrial environments have recently been reported to produce an uncommon cyanotoxin apratoxin, the production of which was previously associated exclusively with marine environments [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, strains isolated from terrestrial environments have recently been reported to produce an uncommon cyanotoxin apratoxin, the production of which was previously associated exclusively with marine environments [ 7 ]. Bioassays could be a great tool to examine the general toxicity of inoculants [ 27 , 73 ], which is especially important for the rehabilitation of degraded and desertified land by cyanobacterial inoculation, since the application of toxic cyanobacterial strains would be hazardous.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Common Cyanotoxins in Cyanobacteria of Biological Loess Crusts

Dulić

Svirčev

Malešević

et al. 2022

Toxins

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Cyanotoxins are a diverse group of bioactive compounds produced by cyanobacteria that have adverse effects on human and animal health. While the phenomenon of cyanotoxin production in aquatic environments is well studied, research on cyanotoxins in terrestrial environments, where cyanobacteria abundantly occur in biocrusts, is still in its infancy. Here, we investigated the potential cyanotoxin production in cyanobacteria-dominated biological loess crusts (BLCs) from three different regions (China, Iran, and Serbia) and in cyanobacterial cultures isolated from the BLCs. The presence of cyanotoxins microcystins, cylindrospermopsin, saxitoxins, and β-N-methylamino-L-alanine was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, while the presence of cyanotoxin-encoding genes (mcyE, cyrJ, sxtA, sxtG, sxtS, and anaC) was investigated by polymerase chain reaction (PCR) method. We could not detect any of the targeted cyanotoxins in the biocrusts or the cyanobacterial cultures, nor could we amplify any cyanotoxin-encoding genes in the cyanobacterial strains. The results are discussed in terms of the biological role of cyanotoxins, the application of cyanobacteria in land restoration programs, and the use of cyanotoxins as biosignatures of cyanobacterial populations in loess research. The article highlights the need to extend the field of research on cyanobacteria and cyanotoxin production to terrestrial environments.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of Common Cyanotoxins in Cyanobacteria of Biological Loess Crusts

Dulić

Svirčev

Malešević

et al. 2022

Toxins

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show abstract

“…Importantly, some cyanobacterial strains exert toxic effects despite not producing any known cyanotoxins, thus indicating the presence of potentially unknown or uncharacterized toxins. In fact, there are numerous experimental works that show the neurotoxic, hepatotoxic, and cytotoxic action of cyanobacterial extracts with no known cyanotoxins [ 14 , 15 , 16 , 17 ], underscoring the need to discover and characterize potential new toxins and/or bioactive compounds produced by HABs. In interesting research conducted by Spoof et al, the researchers have isolated and identified new bioactive, cyclic hexapeptides—Anabaenopeptins—from a cyanobacterial bloom extract in the Baltic Sea and found the compounds to inhibit the activity of protein phosphatase 1 and carboxypeptidase A but no inhibition of chymotrypsin, trypsin, or thrombin [ 18 ].…”

Section: Harmful Algal Blooms and Cyanotoxinsmentioning

confidence: 99%

As We Drink and Breathe: Adverse Health Effects of Microcystins and Other Harmful Algal Bloom Toxins in the Liver, Gut, Lungs and Beyond

Lad

Breidenbach

et al. 2022

Life

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Freshwater harmful algal blooms (HABs) are increasing in number and severity worldwide. These HABs are chiefly composed of one or more species of cyanobacteria, also known as blue-green algae, such as Microcystis and Anabaena. Numerous HAB cyanobacterial species produce toxins (e.g., microcystin and anatoxin—collectively referred to as HAB toxins) that disrupt ecosystems, impact water and air quality, and deter recreation because they are harmful to both human and animal health. Exposure to these toxins can occur through ingestion, inhalation, or skin contact. Acute health effects of HAB toxins have been well documented and include symptoms such as nausea, vomiting, abdominal pain and diarrhea, headache, fever, and skin rashes. While these adverse effects typically increase with amount, duration, and frequency of exposure, susceptibility to HAB toxins may also be increased by the presence of comorbidities. The emerging science on potential long-term or chronic effects of HAB toxins with a particular emphasis on microcystins, especially in vulnerable populations such as those with pre-existing liver or gastrointestinal disease, is summarized herein. This review suggests additional research is needed to define at-risk populations who may be helped by preventative measures. Furthermore, studies are required to develop a mechanistic understanding of chronic, low-dose exposure to HAB toxins so that appropriate preventative, diagnostic, and therapeutic strategies can be created in a targeted fashion.

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“…These findings indicated that MCs could cause varied influences on the antioxidant system and the innate immune system in fish, which may greatly depend on the exposure routes and concentrations/doses. Furthermore, Falfushynska et al [84] demonstrated that 20 µg/L MC-LR could induce multifaceted toxic effects including oxidative stress and apoptosis in zebrafish, which characterized by the increase in thiobarbituric acid-reactive substances (TBARS) levels and caspase-3a (cas3a) and caspase-3b (cas3b).…”

Section: Pure Microcystinsmentioning

confidence: 99%

Microcystin-Induced Immunotoxicity in Fishes: A Scoping Review

Wang

Hung

Kurobe

et al. 2021

Toxins

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Cyanobacteria (blue-green algae) have been present on Earth for over 2 billion years, and can produce a variety of bioactive molecules, such as cyanotoxins. Microcystins (MCs), the most frequently detected cyanotoxins, pose a threat to the aquatic environment and to human health. The classic toxic mechanism of MCs is the inhibition of the protein phosphatases 1 and 2A (PP1 and PP2A). Immunity is known as one of the most important physiological functions in the neuroendocrine-immune network to prevent infections and maintain internal homoeostasis in fish. The present review aimed to summarize existing papers, elaborate on the MC-induced immunotoxicity in fish, and put forward some suggestions for future research. The immunomodulatory effects of MCs in fish depend on the exposure concentrations, doses, time, and routes of exposure. Previous field and laboratory studies provided strong evidence of the associations between MC-induced immunotoxicity and fish death. In our review, we summarized that the immunotoxicity of MCs is primarily characterized by the inhibition of PP1 and PP2A, oxidative stress, immune cell damage, and inflammation, as well as apoptosis. The advances in fish immunoreaction upon encountering MCs will benefit the monitoring and prediction of fish health, helping to achieve an ecotoxicological goal and to ensure the sustainability of species. Future studies concerning MC-induced immunotoxicity should focus on adaptive immunity, the hormesis phenomenon and the synergistic effects of aquatic microbial pathogens.

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Multibiomarker-based assessment of toxicity of central European strains of filamentous cyanobacteria Aphanizomenon gracile and Raphidiopsis raciborskii to zebrafish Danio rerio

Cited by 20 publications

References 90 publications

Assessment of Common Cyanotoxins in Cyanobacteria of Biological Loess Crusts

Assessment of Common Cyanotoxins in Cyanobacteria of Biological Loess Crusts

As We Drink and Breathe: Adverse Health Effects of Microcystins and Other Harmful Algal Bloom Toxins in the Liver, Gut, Lungs and Beyond

Microcystin-Induced Immunotoxicity in Fishes: A Scoping Review

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