Multicenter evaluation of an enzymatic method for glycated albumin

Paleari, Renata; Bonetti, Graziella; Callà, Cinzia Anna Maria; Carta, M.; Ceriotti, Ferruccio; Gaetano, Nicola Di; Ferri, Marilisa; Guerra, Elena; Lavalle, Gabriella; Cascio, Claudia Lo; Martino, Francesca Gabriela; Montagnana, Martina; Moretti, Marco; Santini, Gabriele; Scribano, Donata; Testa, Roberto; Vero, Anna; Mosca, Andrea

doi:10.1016/j.cca.2017.03.028

Cited by 20 publications

(17 citation statements)

References 27 publications

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“…At present, enzymatic assays to measure GA are well standardized and have been automated for high throughput analysis [6,20,21,[23][24][25]. Lucica GA-L® was the first enzymatic methodology launched on the market [8].…”

Section: Discussionmentioning

confidence: 99%

Factors associated with glycated albumin in adults without diabetes

Freitas¹,

Hernandez²,

Camargo³

2020

Medicine and Pharmacy Reports

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Background and aims. Glycated albumin is a glycemic marker useful in short-term monitoring and in situations when a glycated hemoglobin test is not reliable. This study aims to evaluate glycated albumin levels and its associated factors in normoglycemic adults from Southern Brazil. Method. 136 individuals, without diabetes or pre-diabetes, were included in this cross-sectional study. Levels of glycated albumin, glycated hemoglobin, and other biochemical markers were measured. Results. Glycated albumin levels ranged from 11.1% to 17.5% (2.5th and 97.5th percentiles). Glycated albumin/glycated hemoglobin ratio was 2.8 ± 0.2. Glycated albumin did not differ according to gender and age groups. However, in overweight individuals, levels of glycated albumin and glycated albumin/glycated hemoglobin ratio were lower and weakly and negatively correlated with body mass index. Conclusions. Glycated albumin levels in Brazilians were similar to those previously described in other populations. Glycated albumin seems to be irrespective of gender or age, but weakly correlated with weight. These aspects should be taken into account in the interpretation of glycated albumin results.

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Section: Discussionmentioning

confidence: 99%

Factors associated with glycated albumin in adults without diabetes

Freitas¹,

Hernandez²,

Camargo³

2020

Medicine and Pharmacy Reports

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“…GA was measured using a standardized enzymatic quantitative method (QuantILab®, Instrumentation Laboratory SpA - A Werfen Company, Milan, Italy) and was reported as a percentage of total albumin measured in the same serum sample, minimizing the effects of variations among patients and in albumin concentrations. The percentage of GA was obtained by a math calculation: glycated albumin/total albumin x 100 [9]. Creatinine was measured by a colorimetric method and glomerular filtration rate was estimated using CKD-EPI equation by accessing www.mdrd.com.…”

Section: Methodsmentioning

confidence: 99%

Glycated albumin is correlated with glycated hemoglobin in type 2 diabetes

Ciobanu

Bogdan²,

Pătruţ³

et al. 2019

Medicine and Pharmacy Reports

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Background and aims. Glycated hemoglobin (HbA1c) retrospectively evaluates mean glycemia in the preceding 2-3 months and is the gold standard for assessing glycemic control, while glycated albumin (GA) is currently considered a short to intermediate term integrated glycemic control marker, since it reflects glycemic status over the last 3 weeks. We aimed to investigate the levels of GA, HbA1c and fasting glycemia in a group of patients with type 2 diabetes. Methods. The observational study included adult type 2 diabetes patients (n=135) according to inclusion and exclusion criteria, randomly selected from Clinical Centre of Diabetes, Cluj-Napoca, Romania. Fasting glycemia, GA, HbA1c and creatinine were measured using commercially available methods. Results. Of the whole group, 62 (45.9 %) were men. Mean age was 62.1±8.6 years old, body mass index was 31.8±6.1 kg/m2 and diabetes duration was 10.0 (4.0; 15.0) years. Fasting glycemia was 162±13.7 mg/dl, GA was 28.0 (21.0; 40.0)% and HbA1c 8.9±2.3%. We found GA was significantly correlated with HbA1c (r=0.19; p=0.029) and fasting glycemia (r=0.32; p<0.001), while HbA1c was significantly correlated with fasting glycemia (r=0.40; p<0.001). Conclusions. GA was significantly correlated with both HbA1c and fasting glycemia in our patients with type 2 diabetes. While HbA1c is recognized as being the reference test for diabetes control monitoring, GA might a useful biomarker for assessing short to intermediate term glycemic control, particularly important in situations when HbA1c test cannot be reliable or earlier clinical decision making is mandatory.

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“…Glycated albumin was determined in all serum and plasma samples with quantILab Glycated Albumin (Instrumentation Laboratory SpA - A Werfen Company, Milan, Italy) on Architect c8000 platform (Abbott Italia Abbott SRL, Rome, Italy), after calibration with ReferrIL Glycated Albumin (Instrumentation Laboratory SpA - A Werfen Company, Milan, Italy) ( 7 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of different anticoagulants on glycated albumin quantification

Bonetti

Gaetano²,

Paleari

et al. 2018

Biochem. med. (Online)

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IntroductionIn the last 20 years glycated albumin (GA) measurement has been demonstrated to be a reliable glycation marker and recently as the most innovative one in western countries. Glycated albumin has been already adopted by some Asian countries due to its usefulness in diabetes screening. The aim of the present study was to investigate for the first time the effects of different anticoagulants on GA assay.Materials and methodsFrom each of 60 patients a serum tube and K3EDTA, Li-Heparin and NaF-EDTA containing tubes were collected. All tubes were from Sarstedt (Verona, Italy). Glycated albumin was measured in duplicate in each sample tube in a single analytical run with quantILab glycated albumin (Instrumentation Laboratory SpA - A Werfen Company, Milan, Italy) on Architect c8000 analyser (Abbott SRL, Rome, Italy). Comparison of GA% in evaluated tubes was made by paired Wilcoxon test.ResultsMedian and interquartile range GA% concentrations were 15.4% (13.2 - 19.1) in serum, 15.7% (13.6 - 19.9) in K3EDTA, 15.6% (13.3 - 19.7) in Li-heparin and 15.5% (13.1 - 19.3) in NaF-EDTA samples, respectively. Glycated albumin mean relative bias respect to serum was within desirable bias derived from biological variation studies (± 2.9%) when K3EDTA (+ 2.8%), Li-heparin (+ 0.9%) or NaF-EDTA (+ 0.1%), were used as anticoagulants.ConclusionsOur results demonstrate that the GA% assay is not affected by relevant interferences when K3EDTA, Li-heparin or NaF-EDTA are used as anticoagulants, so they can be used interchangeably without a relevant impact on the clinical use of the test.

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Multicenter evaluation of an enzymatic method for glycated albumin

Cited by 20 publications

References 27 publications

Factors associated with glycated albumin in adults without diabetes

Factors associated with glycated albumin in adults without diabetes

Glycated albumin is correlated with glycated hemoglobin in type 2 diabetes

Effects of different anticoagulants on glycated albumin quantification

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