Multicenter Evaluation of the
 Candida albicans
 /
 Candida glabrata
 Peptide Nucleic Acid Fluorescent In Situ Hybridization Method for Simultaneous Dual-Color Identification of
 C. albicans
 and
 C. glabrata
 Directly from Blood Culture Bottles

Shepard, Janeen; Addison, Rachel; Alexander, Barbara D.; Della‐Latta, Phyllis; Gherna, Michael; Haase, G.; Hall, Gerri S.; Johnson, J. Kristie; Merz, William G.; Peltroche-Llacsahuanga, Heidrun; Stender, Henrik; Venezia, Richard A.; Wilson, Deborah A.; Procop, Gary W.; Wu, Fann; Fiandaca, Mark J.

doi:10.1128/jcm.01385-07

Cited by 145 publications

(93 citation statements)

References 24 publications

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“…Many stu di es in dica ted that FISH met hod can iden tify mic ro or ganisms with very high sen si ti vity (100%) and spe ci fi city (100%) and mi xed in fec ti ons can al so be de tec ted by this met hod. [22][23][24] Be si de this, ot her stu - Börekçi ve ark. Enfeksiyon Hastalıkları di es ha ve in di ca ted that PCR-EI A and se mi-nes ted PCR were mo re sen si ti ve than ro u ti ne phe noty pic met hods for the de tec ti on and iden ti fi ca ti on of mic ro or ga nisms in mi xed cul tu res.…”

Section: Discussionmentioning

confidence: 99%

“…21 Se ve ral stu di es ha ve shown that mix tu res of iso la tes in cul tu res can be de tec ted by FISH met hod and FISH is mo re sen siti ve than con ven ti o nal met hods in the de tec ti on of mix tu res of iso la tes in blo od cul tu res. [22][23][24] The aims of this study we re first to in ves ti gate the cli ni cal fe a tu res and risk fac tors in pa ti ents with sing le and mul tip le-spe ci es can di de mi a de tected by FISH met hod. In addition, the epi so des of bre akt hro ugh can di de mi a we re eva lu a ted.…”

Section: Gürol Emekdaşmentioning

confidence: 99%

See 1 more Smart Citation

The High Rate of Multiple-Species Candidemia Detected with Fluorescence In Situ Hybridization (FISH) Methods: What are the Clinical and Prognostic Implications?

Börekçi¹,

Ersöz²,

Göksu³

et al. 2010

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : The flu o res cen ce in si tu hybri di za ti on (FISH) with rRNA-tar ge ted pro bes is a mo le cu lar met hod that al lows iden ti fi ca ti on of mic ro or ga nisms in mi xed as semb la ges from di rect cli ni cal samp les wit ho ut cul ti vi ta ti on. The aim of this study was to in ves ti ga te the cli ni cal fe a tu res and risk fac tors in pa ti ents with sing le and mul tip le-spe ci es can di de mi a de tec ted with FISH met hod and al so to eva lu a te the epi so des of bre akt hro ugh can di de mi a. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : A to tal of 325 po si ti ve blo od cul tu re samp les we re exa mi ned bet we en Ja nuary and Au gust 2004 in me di cal mic ro bi o logy la bo ra tory of Mer sin Uni ver sity Hos pi tal. The de mog rap hic and clini cal da ta of 45 pa ti ents with can di de mi a de tec ted with con ven ti o nal cul tu re met hods, FISH and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) we re analy zed ret ros pec ti vely, sing le and mul tip le-spe ci es can di de mi a we re in ves ti ga ted among pa ti ents with can di de mi a and the se pa ti ents we re com pared with res pect to risk fac tors. R Re e s su ul lt ts s: : Thirty fi ve (77.8%) of 45 pa ti ents with can di de mi a had a sing le Can di da spe ci es and 10 (22.2%) had mul tip le-spe ci es de tec ted by FISH met hod. Of the se 45 ca ses, 31.1% had as so ci a ted so lid and he ma to lo gic ma lig nancies. The me an du ra ti on of hos pi ta li za ti on was 27 (2-180) days. All pa ti ents had at le ast one ind wel ling cat he ter (uri nary cat he ter: 80%, pe rip he ric ve no us cat he ter: 55.6%, cen tral ve no us cat heter: 26.7%). Most of the pa ti ents (95.6%) had be en tre a ted with bro ad spec trum an ti bi o tics. The can di de mi as were mostly ca u sed by non-al bi cans Can di da spp., par ti cu larly Can di da pa rap si lo sis (71.1%). The ra te of bre akt hro ugh can di de mi a was de tec ted in 11.1% of the pa ti ents with can di de mi a and all of the se we re in pa ti ents with sing le spe ci es can di de mi a. Sur pri singly, in ca se of sing le-spe ci es can di de mi a, the mor ta lity ra te (77.8%) was hig her than that of mul tip le-spe ci es can di de mi a (22.2%). C Co on nc c l lu u s si i o on n: : Ac cor ding to our da ta, the ind wel ling cat he ters and tre at ment with mul tip le an ti bi o tics we re con si de red as the risk fac tors for sing le and mul tip le-spe ci es can di demi a. Alt ho ugh the ra te of mul tip le-spe ci es can di de mi a de tec ted with FISH met hod was fo und to be high, cli nical and prog nos tic sig ni fi can ce of mul tip le-spe ci es can di de mi a are still de ba tab le. K Ke ey y W Wo or rd ds s: : Fun ge mi a; can di da; in si tu hybri di za ti on, flu o res cen ce Ö ÖZ ZE ET T A Am ma aç ç: : Flu o re san in si tu hib ri di zas yon (FISH) rRNA' yı he def alan prob lar kul la na rak kül tür yön tem le ri ne baş vur mak sı zın di rekt kli nik ör nek ler de...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Gürol Emekdaşmentioning

confidence: 99%

The High Rate of Multiple-Species Candidemia Detected with Fluorescence In Situ Hybridization (FISH) Methods: What are the Clinical and Prognostic Implications?

Börekçi¹,

Ersöz²,

Göksu³

et al. 2010

Turkiye Klinikleri J Med Sci

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show abstract

“…A newer version of this test now allows for the simultaneous identification of either C albicans or C glabrata [16,17].…”

Section: Candidiasismentioning

confidence: 99%

“…• Nonculture Candida detection assays [18][19][20] • The Candida mannan assay yields a sensitivity of 31-90% (less for non-albicans Candida species).…”

Section: Candidiasismentioning

confidence: 99%

Fungal Infections in Immunosuppressed Patients

Puebla¹

2012

Immunodeficiency

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“…Recently, ISH techniques employing PNA probes for rRNA have been developed as useful techniques for the differentiation of medically important Candida spp. (11,23). These novel properties enabled PNA probes to hybridize to complementary nucleic acid targets with high specificity and rapid binding kinetics (24,25).…”

mentioning

confidence: 99%

Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections

et al. 2013

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In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.

show abstract

Multicenter Evaluation of the Candida albicans / Candida glabrata Peptide Nucleic Acid Fluorescent In Situ Hybridization Method for Simultaneous Dual-Color Identification of C. albicans and C. glabrata Directly from Blood Culture Bottles

Cited by 145 publications

References 24 publications

The High Rate of Multiple-Species Candidemia Detected with Fluorescence In Situ Hybridization (FISH) Methods: What are the Clinical and Prognostic Implications?

The High Rate of Multiple-Species Candidemia Detected with Fluorescence In Situ Hybridization (FISH) Methods: What are the Clinical and Prognostic Implications?

Fungal Infections in Immunosuppressed Patients

Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections

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