Multicenter Study To Evaluate Bloodstream Infection by
            <i>Helicobacter cinaedi</i>
            in Japan

Matsumoto, Tetsuya; Goto, Mieko; Murakami, Hiroatsu; Tanaka, Takashi; Nishiyama, Hiroyuki; Ono, Emi; Okada, Chikako; Sawabe, Etsuko; Yagoshi, Michiko; Yoneyama, Akio; Okuzumi, Katsuko; Tateda, Kazuhiro; Misawa, Naoaki; Yamaguchi, Keizo

doi:10.1128/jcm.00465-07

Cited by 81 publications

(86 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been suggested that H. cinaedi is part of the normal intestinal flora in rodents, which might be a reservoir for human infections (8,10). There are also several papers reporting that direct contact with pets may be a possible route of infection in humans (3,4,13,44); however, details regarding the pathogenesis and epidemiological features, including routes of infections of animal isolates in the context of humans and animals, are not fully understood. Among the animal strains examined in this study, only strain 94105 was isolated from a diseased animal (a 2-month-old female puppy with bloody diarrhea) (26).…”

Section: Discussionmentioning

confidence: 99%

“…This microorganism was first isolated from homosexual men as a Campylobacter-like organism (CLO) and was initially recognized as a rectal and intestinal pathogen among members of this population (1). However, H. cinaedi is now considered to be an opportunistic pathogen that causes bacteremia, cellulitis, septicemia, and enteritis in immunocompromised patients (2)(3)(4), immunocompetent patients, and even healthy individuals (5)(6)(7).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Rapid Identification and Subtyping of Helicobacter cinaedi Strains by Intact-Cell Mass Spectrometry Profiling with the Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

Self Cite

View full text Add to dashboard Cite

Helicobacter cinaedi infection is recognized as an increasingly important emerging disease in humans. Although H. cinaedi-like strains have been isolated from a variety of animals, it is difficult to identify particular isolates due to their unusual phenotypic profiles and the limited number of biochemical tests for detecting helicobacters. Moreover, analyses of the 16S rRNA gene sequences are also limited due to the high levels of similarity among closely related helicobacters. This study was conducted to evaluate intact-cell mass spectrometry (ICMS) profiling using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) as a tool for the identification of H. cinaedi. A total of 68 strains of H. cinaedi isolated from humans, dogs, a cat, and hamsters were examined in addition to other Helicobacter species. The major ICMS profiles of H. cinaedi were identical and differed from those of Helicobacter bilis, which show >98% sequence similarity at the 16S rRNA sequence level. A phyloproteomic analysis of the H. cinaedi strains examined in this work revealed that human isolates formed a single cluster that was distinct from that of the animal isolates, with the exception of two strains from dogs. These phyloproteomic results agreed with those of the phylogenetic analysis based on the nucleotide sequences of the hsp60 gene. Because they formed a distinct cluster in both analyses, our data suggest that animal strains may not be a major source of infection in humans. In conclusion, the ICMS profiles obtained using a MALDI-TOF MS approach may be useful for the identification and subtyping of H. cinaedi.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Rapid Identification and Subtyping of Helicobacter cinaedi Strains by Intact-Cell Mass Spectrometry Profiling with the Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although Helicobacter species are recognized as major pathogens associated with gastrointestinal tract infection, H. cinaedi is commonly isolated from compromised patients. [6][7][8]10) However, there have been increasing reports of infection in immunocompetent patients in whom H. cinaedi has been isolated from blood and caused recurrent bacteremia and latent or chronic infections. [6][7][8]10) Recently Lewis, et al 9) reported isolation of H. cinaedi from an immunocompetent patient with myopericarditis.…”

Section: Discussionmentioning

confidence: 99%

“…[2][3][4][5] Helicobacter cinaedi is commonly isolated from compromised patients; however, there are increasing reports of this organism infecting immunocompetent patients. [6][7][8] H. cinaedi infection is reportedly associated with cardiovascular disease. 9,10) We here report a case of a pericoronary pseudotumor caused by H. cinaedi.…”

mentioning

confidence: 99%

Pericoronary Pseudotumor Caused by Helicobacter Cinaedi

et al. 2014

View full text Add to dashboard Cite

SummaryCardiac tumors and tumor-like lesions are uncommon; most are true neoplasms. We here report a case of a pericoronary tumor-like lesion surrounding the right coronary artery in a 39-year-old man who presented with fever and chest pain. Although clarithromycin was administered for 1 week, his fever persisted. Helicobacter cinaedi (H. cinaedi) was isolated from blood cultures and found to be sensitive to ceftriaxone. A computed tomography scan showed a tumor-like lesion with no 18 F-fluorodeoxyglucose uptake surrounding the right coronary artery. After administration of ceftriaxone, the tumor-like lesion diminished in size according to meticulous computed tomography examinations. We therefore concluded that it was caused by H. cinaedi infection. The patient has been followed up closely for 1 year and remains asymptomatic. (Int Heart J 2014; 55: 463-465) Key words: Cardiac tumor, PET-CT P rimary cardiac tumors are uncommon; most are neoplasms. However, some tumor-like lesions are reactive, pseudoneoplastic masses. 1) Possible pathogenetic mechanisms include inflammation, autoimmune disorders, and infections.2-5) Helicobacter cinaedi is commonly isolated from compromised patients; however, there are increasing reports of this organism infecting immunocompetent patients. [6][7][8] H. cinaedi infection is reportedly associated with cardiovascular disease.9,10) We here report a case of a pericoronary pseudotumor caused by H. cinaedi. Case ReportA 39-year-old man presented to another institution with fever and chest pain. His white blood cell count was 19,440/ μL, and serum C-reactive protein concentration 11.2 mg/dL. Pneumonia was suspected and 400 mg of clarithromycin per day was administered orally for 1 week. However, his fever persisted and H. cinaedi was isolated from blood cultures, after which 2 g per day of intravenous ceftriaxone was substituted for clarithromycin for 2 weeks. Because infective endocarditis was suspected, transthoracic echocardiography was performed. This showed a mass on the lateral side of the right atrium and ventricle. A computed tomography (CT) scan showed a 5.8 × 4.2 cm tumor-like lesion around the right coronary artery (Figure 1). After he had received ceftriaxone for 2 weeks, the results of blood examinations returned to within normal limits and he was referred to our hospital for a suspected cardiac tumor.On presentation to our hospital, his physical examination was normal. White blood cell count was 4,720/μL with a normal differential count and the C-reactive protein concentration was within normal limits. Biochemical data, including serum immunoglobulin concentrations, were unremarkable. Tumor markers such as carcinoembryonic antigen, cancer antigens 19-9 and 125, α-fetoprotein, squamous cell carcinoma antigen, interleukin-6, and soluble interleukin-2R were all within normal limits. There was no evidence of immune deficiency and HIV serology was negative. Chest X-ray films showed no cardiomegaly or any other abnormalities. Electrocardiography showed normal sinus rhythm with...

show abstract

“…Later in 1991, on the basis of hybridization data, this organism was reclassified as belonging to the genus Helicobacter (2). Although H. cinaedi infections previously were reported mainly in immunocompromised patients (3)(4)(5)(6)(7), recently increasing numbers of infection have been reported in immunocompetent patients (8,9). Examples of the diverse range of infections caused by H. cinaedi include proctocolitis, gastroenteritis, neonatal meningitis, localized pain, rash and bacteremia (10).…”

mentioning

confidence: 99%

Comparative evaluation of the agar dilution and broth microdilution methods for antibiotic susceptibility testing ofHelicobacter cinaedi

Tomida

Oumi

Okamoto

et al. 2013

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.

show abstract

Multicenter Study To Evaluate Bloodstream Infection by Helicobacter cinaedi in Japan

Cited by 81 publications

References 17 publications

Rapid Identification and Subtyping of Helicobacter cinaedi Strains by Intact-Cell Mass Spectrometry Profiling with the Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Rapid Identification and Subtyping of Helicobacter cinaedi Strains by Intact-Cell Mass Spectrometry Profiling with the Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Pericoronary Pseudotumor Caused by Helicobacter Cinaedi

Comparative evaluation of the agar dilution and broth microdilution methods for antibiotic susceptibility testing ofHelicobacter cinaedi

Contact Info

Product

Resources

About