Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level

Abstract

The simultaneous detection of multiple microRNAs (miRNAs) will facilitate the early clinical diagnosis. Herein, we demonstrate the integration of multicolor fluorophores-encoded cascade signal amplification with single-molecule detection for simultaneous measurement...

CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging

“…Single-molecule counting is a highly sensitive quantitative method achieved by counting the fluorescent dots. 52–54 This method has the advantage of low sample consumption, based on which our proposed method was carried out to detect mitomiR. This is a new attempt to detect low-abundance mitomiR, which is helpful for subcellular level detection, for example the mitochondrial content.…”

Spatiotemporal Regulation of Metal Ions in the Polymerase Chain Reaction

“…The polymerase chain reaction (PCR) has been widely used in clinical diagnosis and microorganism detection due to its unsurpassable sensitivity, − since its first invention in 1980s . However, in some cases where low templates are required for continuous amplification, such as forensic identification, PCR products exhibit severe tailing bands, i.e., smear, which makes it difficult to obtain clear results. , The solution to these cases is to improve the specificity of PCR.…”

Sensitive detection of fusion transcripts with padlock probe-based continuous cascade amplification (P-CCA)

“…RCA has been widely used on account of the specific and rapid detection of nucleic acid sequences in recent years. [31][32][33][34] However, RCA suffers from low sensitivity and always takes several hours, so it is necessary to integrate it with another reaction to achieve a more specific and fast amplification of the cyclized DNA. Loop-mediated isothermal amplification (LAMP) is another isothermal amplification technique using a strand displacing DNA polymerase.…”

“…Moreover, LAMP-based methods have high specificity due to the design of four special primers to recognize six pre-defined sequences of the target. [37][38][39] LAMP has been adapted in various ways for the detection of single nucleotide polymorphism, 40 DNA methylation, 37 microRNA 32,41 and telomerase activity. 42 However, the design of DNA primers in LAMP is complicated and the length of the target is required to be 140-300 nt.…”