Spatiotemporal Regulation of Metal Ions in the Polymerase Chain Reaction

Zhang, Xianjing; Guo, Jun; Song, Bo; Zhang, Feng

doi:10.1021/acsomega.2c04507

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…In contrast, THz-PCR not only amplified the specific product (target) but also provided the reliable detection results ∼7 cycles in advance, which equals to increasing the concentration of target templates or enhancing the sensitivity of PCR by two orders of magnitude. Compared with the other published methods for enhancing PCR, − THz shining could be an innovated one with its own advantages. For example, the photonic PCR method relies on the efficient photothermal conversion of nanomaterials (gold nanospheres, gold nanorods, magnetic nanoparticles, etc.)…”

Section: Resultsmentioning

confidence: 99%

“…When the denaturation time was extended to 6 s, no significant difference could be distinguishable expect for the reduced yield of nonspecific products, and the results showed no detectable difference as T d was extended to 15 s (Figure S5). These results suggested a spatiotemporal switch 34 involved in PCR, i.e., T d set slightly higher than the T m can unwind dsDNA completely as if the denaturation time is long enough.…”

Section: Spatiotemporal Switch Of Pcr Specificity By Thz Shiningmentioning

confidence: 94%

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THz-PCR Based on Resonant Coupling between Middle Infrared and DNA Carbonyl Vibrations

Zhang

2023

ACS Appl. Mater. Interfaces

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The carbonyl groups of deoxyribonucleotide can resonantly couple with 53 THz middle infrared, which can highly transmit water without ionization-based damage to DNA molecules. Herein, we predict that vibrational coupling with THz irradiation could lower down the hybridization landscape of nucleic acids and thus affect DNA replication. Using polymerase chain reaction (PCR) as a measure, we found that THz shining can reduce the denature temperature of DNA duplexes by about 3 °C, which allows one to conduct PCR at lower temperature, facilitating long-time amplification reaction without losing enzymatic fidelity, i.e., normal PCR should be carried out at denaturing temperature ∼4 °C higher than the melting temperature (T m), but THz-PCR only requires temperature ∼1 °C higher than T m due to the nonthermal effect of THz shining. Moreover, the melting time can also be shortened to 1/5 due to the enhanced vibration coupling with 53 THz irradiation. We proposed THz-PCR as an innovated DNA amplification technique with ultrahigh specificity and sensitivity and also successfully demonstrated its advantages in forensic detections.

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Section: Resultsmentioning

confidence: 99%

Section: Spatiotemporal Switch Of Pcr Specificity By Thz Shiningmentioning

confidence: 94%

THz-PCR Based on Resonant Coupling between Middle Infrared and DNA Carbonyl Vibrations

Zhang

2023

ACS Appl. Mater. Interfaces

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Processing biological samples from simulated radiological terrorist events using Rapid DNA instruments

Frégeau,

Laurin

2024

Forensic Science International

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Structural and Solution Speciation Studies on fac-Tricarbonylrhenium(I) Complexes of 2,2′-Bipyridine Analogues

Pivarcsik,

Kljun,

Clemente Rodriguez

et al. 2024

ACS Omega

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In this study, we report the synthesis and characterization of 12 novel rhenium(I) complexes with the general formula fac-[Re(CO) 3 (NN)X] n+ where (NN) is a 2,2′-bipyridine analogue ligand, X = Cl − , Br − , or H 2 O, and n = 0 or 1, focusing on their speciation in an aqueous solution. The prepared organorhenium complexes are stable in a wide pH range in an aqueous solution, and no release of the bidentate ligands or the carbonyl ligands was observed. The stability of the complexes in various biologically relevant matrices (cell culture medium and real blood serum) was also demonstrated. However, the simultaneous substitution of the halido ligand by water and slow hydrolysis of the ester bonds in the ligands were observed, affecting both the solubility and the lipophilicity of the compounds. The aqua complexes became more lipophilic in the presence of chloride ions, while the hydrophilicity increased significantly with time due to the hydrolysis of the ester bonds, which probably contributed to their weak pharmacological activity. The results also showed kinetically hindered aqueous solvation of the halido complexes and low chloride ion affinity of the aqua complexes. The deprotonation of the coordinated aqua ligand in the complexes occurs in the pH = 7−10 range, leading to significant formation (18−30%) of hydroxido species at pH = 7.4. The halido complexes showed somewhat higher cytotoxicity (IC 50 = 60−99 μM) on human colon adenocarcinoma cancer cells (Colo205 and Colo320) than the corresponding aqua complexes (IC 50 > 100 μM). In all cases, no antibacterial effect was observed (MIC > 100 μM), but some of the complexes showed moderate antiviral activity (IC 50 ∼ 50 μM) on Herpes simplex virus 2.

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Spatiotemporal Regulation of Metal Ions in the Polymerase Chain Reaction

Cited by 4 publications

References 31 publications

THz-PCR Based on Resonant Coupling between Middle Infrared and DNA Carbonyl Vibrations

THz-PCR Based on Resonant Coupling between Middle Infrared and DNA Carbonyl Vibrations

Processing biological samples from simulated radiological terrorist events using Rapid DNA instruments

Structural and Solution Speciation Studies on fac-Tricarbonylrhenium(I) Complexes of 2,2′-Bipyridine Analogues

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