Multicolor spectral karyotyping of rat chromosomes

Buwe, A.; Steinlein, Claus; Köehler, Michael; Bar‐Am, Irit; Katzin, Nirit; Schmid, Michael

doi:10.1159/000076306

Cited by 21 publications

(13 citation statements)

References 18 publications

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“…Previous multicolor spectral analyses of meiotic chromosomes have been carried out in mouse and rat with normal karyotypes [Heng et al, 2001;Buwe et al, 2003]. The work presented here describes the results obtained by spectral karyotyping of mitotic and male diakinetic chromosomes of CD/Cremona hybrid mice carrying a unique system of multiple Robertsonian translocations.…”

mentioning

confidence: 97%

Multicolor Spectral Analyses of Mitotic and Meiotic Mouse Chromosomes Involved in Multiple Robertsonian Translocations. I. The CD/Cremona Hybrid Strain

Schmid

Steinlein²,

Winking³

2015

Cytogenet Genome Res

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Multicolor spectral analysis (spectral karyotyping) was applied to mitotic and male diakinetic chromosomes of hybrid mice carrying a unique system of 18 autosomal Robertsonian translocation chromosomes with alternating arm homologies. Only the autosomes 19 and the XY sex chromosomes are excluded from these Robertsonian translocations. The translocations, previously identified by conventional banding analyses, could be verified by spectral karyotyping. Besides the Robertsonian translocations, no other interchromosomal rearrangements were detected. In diakineses of male meiosis, the 18 metacentric Robertsonian translocation chromosomes form a very large meiotic ‘superring'. The predictable, specific order of the chromosomes along this ‘superring' was completely confirmed by multicolor spectral analysis. In the majority of diakineses analyzed, the free autosomal bivalent 19 and the XY sex bivalent form a conspicuous complex which tightly associates with the 12;14 Robertsonian translocation chromosome in the ‘superring'.

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mentioning

confidence: 97%

Multicolor Spectral Analyses of Mitotic and Meiotic Mouse Chromosomes Involved in Multiple Robertsonian Translocations. I. The CD/Cremona Hybrid Strain

Schmid

Steinlein²,

Winking³

2015

Cytogenet Genome Res

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“…C Chromosome analysis of the cultured cells revealed that the cells are not of human origin. The large acrocentric chromosomes (arrows) and the small metacentric chromosomes (arrowheads) suggest a rat karyotype [67], which D was confirmed by PCR analysis of the cultured neurons.…”

Section: Example 1: Generation Of Human Neurons From Blood-derived Prmentioning

confidence: 65%

Quality of Cell Products: Authenticity, Identity, Genomic Stability and Status of Differentiation

Dittmar

Simann

Zghoul

et al. 2010

Transfus Med Hemother

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Cellular therapies that either use modifications of a patient’s own cells or allogeneic cell lines are becoming in vogue. Besides the technical issues of optimal isolation, cultivation and modification, quality control of the generated cellular products are increasingly being considered to be more important. This is not only relevant for the cell’s therapeutic application but also for cell science in general. Recent changes in editorial policies of respected journals, which now require proof of authenticity when cell lines are used, demonstrate that the subject of the present paper is not a virtual problem at all. In this article we provide 2 examples of contaminated cell lines followed by a review of the recent developments used to verify cell lines, stem cells and modifications of autologous cells. With relative simple techniques one can now prove the authenticity and the quality of the cellular material of interest and therefore improve the scientific basis for the development of cells for therapeutic applications. The future of advanced cellular therapies will require production and characterization of cells under GMP and GLP conditions, which include proof of identity, safety and functionality and absence of contamination.

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“…For other species at a maximum mFISH with whole chromosome painting probes is possible, e.g. for the rat (Rattus norvegicus f. domestica) (Buwe et al, 2003), the chicken (Gallus gallus f. domestica) (Griffin et al, 1999), the dog (Canis lupus familiaris) (Milne et al, 2004) or even a beetle (Nasonia spec.) (Rutten et al, 2004).…”

Section: Fish-banding Methods In Micementioning

confidence: 99%

Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding

Liehr

Starke

Heller

et al. 2006

Cytogenet Genome Res

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During the last decade not only multicolor fluorescence in situ hybridization (FISH) using whole chromosome paints as probes, but also numerous chromosome banding techniques based on FISH have been developed for the human and for the murine genome. This review focuses on such FISH-banding techniques, which were recently defined as ‘any kind of FISH technique, which provide the possibility to characterize simultaneously several chromosomal subregions smaller than a chromosome arm. FISH-banding methods fitting that definition may have quite different characteristics, but share the ability to produce a DNA-specific chromosomal banding’. While the standard chromosome banding techniques like GTG lead to a protein-related black and white banding pattern, FISH-banding techniques are DNA-specific, more colorful and, thus, more informative. For some, even high-resolution FISH-banding techniques the development is complete and they can be used for whole genome hybridizations in one step. Other FISH-banding methods are only available for selected chromosomes and/or are still under development. FISH-banding methods have successfully been applied in research in evolution- and radiation-biology, as well as in studies on the nuclear architecture. Moreover, their suitability for diagnostic purposes has been proven in prenatal, postnatal and tumor cytogenetics, indicating that they are an important tool with the potential to partly replace the conventional banding techniques in the future.

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Multicolor spectral karyotyping of rat chromosomes

Cited by 21 publications

References 18 publications

Multicolor Spectral Analyses of Mitotic and Meiotic Mouse Chromosomes Involved in Multiple Robertsonian Translocations. I. The CD/Cremona Hybrid Strain

Multicolor Spectral Analyses of Mitotic and Meiotic Mouse Chromosomes Involved in Multiple Robertsonian Translocations. I. The CD/Cremona Hybrid Strain

Quality of Cell Products: Authenticity, Identity, Genomic Stability and Status of Differentiation

Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding

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