Multicopper Laccase Mimicking Nanozymes with Nucleotides as Ligands

Abstract

Using nanomaterials to achieve functional enzyme mimics (nanozymes) is attractive for both applied and fundamental research. Laccases are multicopper oxidases highly important for biotechnology and environmental remediation. In this work, we report an exceptionally simple yet functional laccase mimic based on guanosine monophosphate (GMP) coordinated copper. It forms an amorphous metal-organic framework (MOF) material. The ratio of copper and GMP is 3:4 as determined by isothermal titration calorimetry. It has excellent laccase-like activity and converts a diverse range of phenol containing substrates such as hydroquinone, naphthol, catechol and epinephrine. Comparative work shows that the activity is originated from guanosine coordination instead of phosphate binding in GMP. Cu is required and cannot be substituted by other metal ions. At the same mass concentration, the Cu/GMP nanozyme has a higher V and similar K compared to the protein laccase. To achieve the same catalytic efficiency, the cost of the Gu/GMP is ∼2400-fold lower than that of laccase. The Cu/GMP is much more stable at extreme pH, high salt, high temperature and for long-term storage. This is one of the first laccase-mimicking nanozymes, which will find important applications in analytical chemistry, environmental protection, and biotechnology.

Sensitive chemical sensor array based on nanozymes for discrimination of metal ions and teas

“…Nanozymes synthesis was carried out following a protocol described in the published literature . Nucleotide (10 mM, 200 μ), Tris–HCl buffer (100 mM, pH 8.5, 100 μl), CuCl 2 (50 mM, 100 μL) and deionized water were mixed in centrifuge tube.…”

Nanozyme applications in biology and medicine: an overview

“…For example, peroxidase-like activity was used for wastewater treatment by cobalt-doped graphitic carbon nitride (Co-g-C 3 N 4 ) materials [82], as catalase activity for sensitive and fast sensor of detection H 2 O 2 .…”

“…Another catalytic application of Co 3 O 4 is the calcium detection of milk because of increasing significant presence of calcium ion [82]. Also, it can be in peroxidase and oxidase-like activity that is useful for colorimetric and glucose detection [83].…”

“…The Au-Pt nano rods showed multiple enzyme mimetic capabilities. Au-Ag heterogeneous nano rods as nanozyme interfaces with peroxidaselike activity and their application for one-pot analysis of glucose at nearly neutral pH [82]. Also, AgM (M ¼ Au, Pd and Pt) nanostructures have enzyme properties, and a series of silver-based bimetallic nanostructures, i.e.…”

Self‐Assembly of Nucleobase, Nucleoside and Nucleotide Coordination Polymers: From Synthesis to Applications

“…Our group has synthesized nucleoside monophosphate Cu 2+ CPs and studied their laccase‐like activity . These CPs were of microns in size and comprised aggregates of smaller particles (Figure H).…”