Multidimensional colorimetric sensor array for discrimination of proteins

Colorimetric analysis has become of great importance in recent years to improve the operationalization of plasmonic-based biosensors. The unique properties of nanomaterials have enabled the development of a variety of plasmonics applications on the basis of the colorimetric sensing provided by metal nanoparticles. In particular, the extinction of localized surface plasmon resonance (LSPR) in the visible range has permitted the exploitation of LSPR colorimetric-based biosensors as powerful tools for clinical diagnostics and drug monitoring. This review summarizes recent progress in the biochemical monitoring of clinical biomarkers by ultrasensitive plasmonic colorimetric strategies according to the distance- or the morphology/size-dependent sensing modes. The potential of colorimetric nanosensors as point of care devices from the perspective of naked-eye detection is comprehensively discussed for a broad range of analytes including pharmaceuticals, proteins, carbohydrates, nucleic acids, bacteria, and viruses such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The practical suitability of plasmonic-based colorimetric assays for the rapid visual readout in biological samples, considering current challenges and future perspectives, is also reviewed.

Section: Colorimetric Sensors Based On Etching and Growth Of Noble Metal Nanoparticlesmentioning

confidence: 99%

Clinical Applications of Visual Plasmonic Colorimetric Sensing

Mauriz

2020

“…The use of colorimetric sensors is one of the most basic and convenient analytical techniques, being easy and low-cost [18]. The technique allows to identify changes in the surrounding environment without requiring sophisticated equipment and trained manipulators [19].…”

Section: Introductionmentioning

confidence: 99%

“…The technique allows to identify changes in the surrounding environment without requiring sophisticated equipment and trained manipulators [19]. The basis of colorimetric detection is the analysis of the color change caused by the generation of colored compounds (chromophore) from the reaction between the chromogenic reagent and the analyte [18,19].…”

Section: Introductionmentioning

confidence: 99%

“…Among several advantages, colorimetric sensors are attractive and are used for identification of analytes in different areas [20], such as detecting ammonia in aqueous solution or in a gas phase [19], urea in biological solutions [21], biochemical changes in sweat [22], conformational changes in proteins [18], volatile amines released during the deteriorating state of fish [23], ferric ions [24], and discrimination against organophosphate pesticides [25].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Halochromic Polystyrene Nanofibers Obtained by Solution Blow Spinning for Wine pH Sensing

Miranda

Natarelli

Thomazi

et al. 2020

Colorimetric sensors developed by the solution blow spinning (SBS) technique have a rapid response to a variation in different physicochemical properties. In this study, polystyrene nanofibrous (PSNF) mats containing the bromothymol blue (BTB) indicator were obtained by SBS for the pH sensing of wine sample. The incorporation of the indicator did not promote changes in fiber diameter but led to the appearance of beads, allowing for the encapsulation of BTB. The halochromic property of BTB was retained in the PSNF material, and the migration tests showed that the indicator mats presented values below the maximum acceptable limit (10 mg dm−2) established by EU Commission Regulation No. 10/2011 for foods with an alcohol content up to 20%. The present study opens the possibility of applying nanostructured materials to innovative food packaging which, through nanosensory zones, change color as a function of the food pH.

“…However, the method that quantifies the stress-responsive process using a liposome has not been developed yet. Although there are several methods including array-type protein chip [ 20 ], florescence intensity [ 21 ] and circular dichroism (CD) [ 22 ] measurements to monitor stress-induced structural changes of proteins, those are expensive and labor-consuming. In addition, those methods show the limitations for predicting the dynamics, structure deformation or interaction of proteins.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System

Son

Kim

et al. 2018

An immobilized liposome electrode (ILE)-based sensor was developed to quantify conformational changes of the proteins under various stress conditions. The ILE surface was characterized by using a tapping-mode atomic force microscopy (TM-AFM) to confirm surface immobilization of liposome. The uniform layer of liposome was formed on the electrode. The current deviations generated based on the status of the proteins under different stress were then measured. Bovine carbonic anhydrase (CAB) and lysozyme were tested with three different conditions: native, reduced and partially denatured. For both proteins, a linear dynamic range formed between denatured concentrations and output electric current signals was able to quantify conformational changes of the proteins. The pattern recognition (PARC) technique was integrated with ILE-based sensor to perform data analysis and provided an effective method to improve the prediction of protein structural changes. The ILE-based stress sensor showed potential of leveraging the amperometric technique to manifest activity of proteins based on various external conditions.